ERGİN KARİPTAŞ, Şener TULUMOĞLU, BELGİN ERDEM

Antifungal Effects of Lactobacillus spp. bacteria on candida yeast

Otuz adet Lactobacillus, 5-15 yaş grubu çocuk gaitasından MRS agar’da izole edilerek API 50CHL cihazı tanımlanmaları yapılmıştır. Ayrıca 50 adet kan kültürü kaynaklı Candida ise SD agar’da izole edilerek Vitek 2 cihazında YST kiti ile tanımlanmaları yapılmıştır. Lactobacillus’ların Candida’lar üzerindeki antifungal etkileri SD agar’da kuyu difüzyon yöntemi ile tespit edilmiştir. Lactobacillus’lar en fazla C. albicans (M29 ve M36), C. parapsilosis (M25, M26). C. famata (M28) ve C. guilliermondii (M38) mayaları üzerinde antifungal etki göstermiştir. Bu çalışma Lactobacillus’ların, kan kültürlerinden elde edilen Candida izolatları üzerine antifungal etkisinin varlığını göstermektedir.

Anahtar Kelimeler:

Candida albicans, mikoz, probiyotikler, insan, insan hastalıkları, kandida enfeksiyonu, antifungal özellikler, Lactobacillus, Candida parapsilosis

Lactobacillus bakterilerinin Candida’lar üzerine antifungal etkileri

Thirty Lactobacillus were isolated from the stool of 5-15 year-old children using MRS agar and identified by API 50CHL. Also, 50 Candida were isolated from blood cultures using SD agar and identified by Vitek 2 instrument with YST kit. Antifungal effects of Lactobacillus bacteria on Candida were explored using well diffusion method in SD agar. Lactobacillus had the most prominent antifungal effect against on C. albicans (M29, M36), C. parapsilosis (M25, M26), C. famata (M28) and C. guilliermondii (M38) yeasts. This study shows the presence of antifungal effect of Lactobacillus on Candida isolated from blood cultures.

Keywords:

Candida albicans, mycoses, probiotics, man, human diseases, candidosis, antifungal properties, Lactobacillus, Candida parapsilosis,

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