SÜHEYLA TÜRKYILMAZ, Ömer YILDIZ, ERMAN ORYAŞIN, Seyhan KAYNARCA, Bülent BOZDOĞAN

Molecular identification of bacteria isolated from dairy herds with mastitis

Bu çalışmanın amacı, klinik ve subklinik mastitislere neden olan bulaşıcı ve çevresel aerobik patojen bakteriyel etkenlerin sekans analizi kullanılarak identifikasyonlarının yapılmasıdır. Toplam 244 sığır Kalifornia Mastitis Test (CMT), klinik gözlemler ve mikrobiyolojik identifikasyonlar yapılarak mastitis yönünden incelenmiştir. Yüzyirmiüç sığırın 226 meme lobundan alınan sütlere klinik (%11.4) ve subklinik (%88.6) mastitis teşhisi konulmuştur. Bunların 38 (%16.8)’inde herhangi bir bakteri üremez iken; 188 (%83.2) sütten izolasyon yapılmıştır. Kırk iki tür 16S rRNA fragmentleri çoğaltılarak sekans analizi ile identifiye edilmiştir. Staphylococcus aureus (%22.9) en çok izole edilen tür olmakla birlikte bunu Escherichia coli (%10.1), S. chromogenes (%8.5), S. haemoliticus (%7.5) ve Hafnia alvei (%6.4)’nin izlediği belirlenmiştir. Yüzseksensekiz izolatın 43 (%22.9)’ü bulaşıcı ve 145 (%77.1)’i çevresel etkenler olduğu tespit edilmiştir. İzolatların tür düzeyinde doğru bir şekilde identifikasyonlarının yapılmasında sekans analizinin kullanılmasının faydalı ve pratik olduğu; ayrıca uygun antibiyotik seçimine katkı sağlayacağı düşünülmektedir.

Mastitisli sığır sütlerinden izole edilen bakterilerin moleküler identifikasyonu

The purpose of the present study was to identify environmental and contagious aerobic pathogen agents causing bovine clinical and subclinical mastitis using sequencing. A total of 244 cows were studied for the presence of mastitis using California Mastitis Test (CMT), clinical observations and microbiological isolations. Milk samples were obtained from 226 quarters of 123 cows which were diagnosed having clinical (11.4%) and subclinical (88.6%) mastitis. From these milk samples, 38 (16.8%) had no bacterial growth and from remaining 188 samples (83.2%) microorganisms were isolated. A total of 42 species were identified by sequencing amplified 16S rRNA fragments. The most common species were Staphylococcus aureus (22.9%) followed by Escherichia coli (10.1%), S. chromogenes (8.5%), S. haemoliticus (7.5%) and Hafnia alvei (6.4%). Of 188 isolates 43 were found contagious (22.9%), and 145 (77.1%) environmental agents. Use of sequencing offer identification of aetiological agent species level useful and practical that can help to chose a suitable therapeutic agent.

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