Örsan GÜNGÖR, MEHMET KAYA, KUTLAY GÜRBULAK, HASAN ORAL, SEMRA KAYA, CİHAN KAÇAR

Use of GnRH agonist (Desloreline) in combination with $PGF_{2\alpha}$ on the termination of pregnancy in bitches

Araştırmada seksüel siklus evresi vaginal sitoloji ile belirlenerek en uygun zamanda çiftleştirilen on dişi köpek kullanıldı. İlk çiftleştirme gününü takiben 20. günden itibaren yapılan ultrases muayenesi ile gebe olduğu belirlenen köpeklere Suprelorin® boyun derisi altına yerleştirildi. İmplantın yerleştirilmesini takiben 10. günde bir kereye mahsus olmak üzere $PGF_{2\alpha}$ (cloprostenol, 20 μg/kg,) kas içi yolla uygulandı. Uygulama yapılan köpeklerin tümünde gebelik sonlandı. Progesteron değerinin implantın uygulandığı gün (gebeliğin 20-21. günü), ortalama 28.87±11.86 ng/ml iken, abortion tamamlandığında ortalama 0.43±0.31 ng/ml’ye düştüğü belirlendi. İmplant uygulamasından 10 gün sonra enjekte edilen $PGF_{2\alpha}$’yı takiben ortalama 3.9±0,7 günde köpeklerde abortionun tamamlandığı görüldü. Abortionun vajinal akıntının ilk kez görülmesinden sonra 2.3±0.48 gün içinde tamamlandığı belirlendi. Sonuç olarak, uzun etkili GnRH anoloğu olan Deslorelin’in köpeklerde tek doz $PGF_{2\alpha}$ uygulaması ile birlikte kombine edildiğinde gebeliği başarılı olarak sonlandırabildiği belirlendi

Anahtar Kelimeler:

gebelik, GnRH, yavru atma, dişi köpek, köpek, progesteron, prostaglandinler

Köpeklerde gebeliğin sonlandırılması amacıyla GnRH agonisti (Desloreline) ile $PGF_{2\alpha}$ kombinasyonunun Kullanımı

Ten bitches whose sexual cycle periods were determined with vaginal cytology to be mated at appropriate time were used in this study. Desloreline implant (Suprelorin®) was inserted under the dorsal skin of bitches that were determined to be pregnant using ultrasonography 20 days after the first day of mating. Ten days after the implant insertion, single dose $PGF_{2\alpha}$ (cloprostenol, 20 μg/kg, IM) was administered. The pregnancy was terminated on all bitches that received this procedure. It was determined that the level of progesterone decreased from an average of 28.87±11.86 ng/ml on the day of implant insertion (day 20-21 of pregnancy) to 0.43±0.31 ng/ml when the abortion was completed. It was observed that after the implant insertion, the abortion was completed in an average of 3.9±0.7 days following $PGF_{2\alpha}$ administration. It has been observed that abortions was completed in an average of 2,3±0.48 days after the first vaginal discharge was seen. In conclusion, it was determined that Desloreline, a GnRH agonist, successfully could terminate pregnancy when it is combined with a single dose of $PGF_{2\alpha}$ administration in bitches.

Keywords:

pregnancy, GnRH, abortion, bitches, dogs, progesterone, prostaglandins,

___

1. Feldman EC, Nelson RW: Breeding, pregnancy and parturition. In, Feldan EC, Nelson RW (Ed): Canine and Feline Endocrinology and Reproduction. 1st ed., pp. 547-572, W.B. Saunders Company, Philadelphia, 1997.

2. Onclin K, Verstegen JP, Concannon PW: Time-related changes in canine luteal regulation: in vivo effects of LH on progesterone and prolactin during pregnancy. J Reprod Fertil, 118, 417-424, 2000.

3. Wanke MM, Romagnoli S, Verstegen J, Concannon PW: Pharmacological approaches to pregnancy termination in dogs and cats including the use of prostaglandins, dopamine agonists, and dexamethasone. www.ivis.org, Accessed: 13 August, 2002.

4. Johnston SD, Kustritz MVR, Olson PNS: Canine and Feline Theriogenology. 3rd ed., pp. 6-104, W.B. Saunders Co, Philadelphia, 2004.

5. Johnston SD, Kustritz MVR Olson PNS: Canine and Feline, Theriogenology. 3rd ed., pp. 168-192, W.B. Saunders Co, Philadelphia, 2004.

6. Wanke M, Loza M, Monachesi N, Concannon P: Clinical use of dexametasone for termination of unwanted pregnancy in dogs. J Reprod Fert Suppl, 51, 233-238, 1997.

7. Palmer CW, Post K: Prevention of pregnancy in the dog with a combination of prostaglandin $F_{2\alpha}$ and bromocriptine. Can Vet J, 43, 460-462, 2002.

8. Eilts BE: Pregnancy termination in the bitch and queen. Clin Tech Small Anim Pract, 17, 116-123, 2002.

9. Concannon PW: Contraception in dogs and cats. 29th World Congress of the World Small Anim Vet Assoc, Rodos, Greece, 2004.

10. Kalender H, Beceriklisoy HB, Kanca H, Findik M, Erunal- Maral N, Handler J, Aslan S: Plasma concentrations of folic acid, vitamin B12 and progesterone of cyclic bitches, bitches during pregnancy and induced abortion and bitches with pyometra. Dtsch Tierarztl Wochenschr, 3, 341-344, 2006.

11. Kutzler M, Wood A: Non-surgical methods of contraception and sterilization. Theriogenology, 66, 514-525, 2006.

12. Gobello C: New GnRH analogs in canine reproduction. Anim Reprod Sci, 100, 1-13, 2007.

13. Bertschinger HJ, Asa CS, Calle PP, Long JA, Bauman K, DeMatteo K, Jochle W, Trigg TE, Human A: Control of reproduction and sex related behaviour in exotic wild carnivores with the GnRH analogue deslorelin: Preliminary observations. J Reprod Fertil Suppl, 57, 275-283, 2001.

14. Trigg TE, Wright PJ, Armour AF, Williamson PE, Junaidi A, Martin GB, Doyle AG, Walsh J: Use of a GnRH analogue implant to produce reversible long-term suppression of reproductive function in male and female domestic dogs. J Reprod Fertil Suppl, 57, 255-261, 2001.

15. Herbert CA, Trigg TE: Applications of GnRH in the control and management of fertility in female animals. Anim Reprod Sci, 88, 141-153, 2005.

16. Padula AM: GnRH analogues-agonists and antagonists. Anim Reprod Sci, 88, 115-126, 2005.

17. Wright PJ, Verstegen JP, Onclin K, Jochle W, Armour AF, Martin GB, Trigg TE: Suppression of the oestrous responses of bitches to the GnRH analogue deslorelin by progestin. J Reprod Fertil Suppl, 57, 263-268, 2001.

18. Padula AM, Macmillan KL: Restoration patterns for luteinizing hormone and ovarian function following treatment with GnRH agonist implants (deslorelin) for 7, 14 or 21 days in cycling dairy cows. Anim Reprod Sci, 87, 11-24, 2005.

19. Schultze-Mosgau A, Griesinger G, Altgassen C, Von Otte S, Hornung D, Diedrich K: New developments in the use of peptide gonadotropin-releasing hormone antagonists versus agonists. Expert Opin Investig Drugs, 14, 1085-1097, 2005.

20. Alaçam E: Köpek ve Kedilerde Üreme Süreci ve Sorunları. Birinci Baskı. 25-34, Medisan Yayınevi, Ankara, 2008.

21. Romagnoli SE., Cela M, Camillo F: Use of prostaglandin F2 alpha for early pregnancy termination in the mismated bitch. Vet Clin North Am: Small Anim Pract, 21, 487-499, 1991.

22. Onclin K, Verstegen JP: Comparisons of different combinations of analogues of PGF2 alpha and dopamine agonists for the termination of pregnancy in dogs. Vet Rec, 144, 416-419, 1999.

23. Schäfer-Somi S, Aksoy OA, Beceriklisoy HB, Einspanier A, Hoppen HO, Aslan S: Repeated induction of abortion in bitches and the effect on plasma concentrations of relaxin, progesterone and estradiol-17beta. Theriogenology, 68, 889-895, 2007.