MİNE YAMAN, ALİ BAYRAKDAR

An immunohistochemical study of the insulin-, Glucagon-, orexin A- and ghrelin- immunoreactive cells in the endocrine pancreas of guinea pig

Bu çalışma, kobay pankreatik adacıklarında immunohistokimyasal metodlar kullanarak insülin, glukagon, orexin A (OXA) ve ghrelin salgılayan endokrin hücrelerin bölgesel dağılımını ve nisbi frekansını ortaya koymak için yapıldı. İnsülin-IR hücrelerin çoğu pankreatik adacıkların merkez bölgesinde, kısmen de manto bölgesinde belirlendi. Glukagon-IR hücreler genellikle pankreatik adacıkların periferinde, çok az yoğunlukta ise merkez bölgede tespit edildi. OXA ve ghrelin-IR hücreler ise pankreatik adacıkların merkezi ve periferal bölgelerine lokalize oldu. Daha açık ifadeyle, OXA-IR hücrelerin lokalizasyonu glukagon-IR hücrelere benzerlik gösterdi. Buna karşın, ghrelin immunoreaktivitesi insülin-IR hücrelerinkinden farklı bir dağılım sergiledi. Sonuç olarak; kobayların pankreatik adacıklarındaki endokrin hücrelerin karekteristik dağılımı türe özgü farklılık göstermektedir.

Anahtar Kelimeler:

immünohistokimya, hormon salınımı, endokrin bezler, glukagon, hormonal kontrol, kobay, pankreas, deney hayvanları, insülin

Kobay endokrin pankreasında insülin-, Glukagon-, orexin A- ve ghrelin- immunoreaktif hücrelerin immunohistokimyasal çalışması

The present study was conducted to clarify the regional distribution and relative frequency of endocrine cells secreting insulin, glucagon, orexin A (OXA) and ghrelin in the pancreatic islets of guinea pig, by specific immunohistochemical methods. In the pancreatic islets, most of insulin-IR cells were detected in the central region, partially in mantle region. Glucagon-IR cells were located in the periphery of the pancreatic islets. In addition, they had lower frequency in the central region. OXA and ghrelin- IR cells were identifed in central and peripheral regions of the pancreatic islets. Clearly, OXA-IR cells exhibited a characteristic distribution pattern resemble to those for the glucagon- IR cells. However, ghrelin immunoreactivity exhibited a characteristic distribution pattern different from that of insulin-immunoreactive cells. In conclusion, some species-dependent unique distributions characteristics of endocrine cells in the pancreatic islets were observed in the guinea pig.

Keywords:

immunohistochemistry, hormone secretion, endocrine glands, glucagon, hormonal control, guineapigs, pancreas, laboratory animals, insulin,

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