ABDULLAH DOĞAN, PINAR AKSU KILIÇLE, DİNÇER ERDAĞ, Murat BAYEZİT, Ertan DOĞAN, Kadir ÖZCAN

Farelerde 3-metilkolantren ile indüklenen fibrosarkoma üzerine sisteaminin etkileri

Tümörlerin biyolojilerinin anlaşılması, korunma ve tedavi yöntemlerinin geliştirilmesi büyük önem arz etmektedir. Bu çalışmada farelerde 3-metilkolantrenle (3-MC) indüklenen fibrosarkoma üzerinde sisteaminin koruyucu etkileri araştırıldı. Deneyde yaklaşık 20±2.0 g ağırlığında beyaz erkek fareler (Mus musculus albino) kullanıldı. Fareler her grupta 15 adet olacak şekilde beş gruba ayrıldı, standart diyet ve su ile ad libitum olarak beslendi. Birinci gruptaki hayvanlara hiçbir ilaç uygulaması yapılmadı. İkinci gruptaki farelere susam yağı (0.2 ml, deri altı), üçüncü gruba sisteamin (%0.1 suda oral), dördüncü gruptaki farelere 3-metilkolantren çözeltisi (1 mg/0.2 ml susam yağı) 0.2 ml hacimde deri altı yolla enjekte edildi. Beşinci gruba 3-metilkolantren çözeltisi (1 mg/0.2 ml susam yağı) 0.2 ml deri altı ve %0.1 oranında suda çözdürülmüş sisteamin oral yolla ad libitum olarak verildi. Hayvanlar 4 ay süreyle takip edildi. Süre sonunda servical dislokasyonla ötanazi edilen farelerin otopsileri yapıldı. Organlardaki morfolojik değişiklikler ve alınan doku örneklerindeki tümöral oluşumlar histopatolojik yöntemlerle araştırıldı. Araştırma sonucunda sisteaminin farelerde 3-metilkolantrenle indüklenen fibrosarkoma karşı koruyucu etki gösterdiği belirlendi.

Anahtar Kelimeler:

sistamin, deney hayvanları, doku dağılımı, sarkom, farmakoloji, farmakokinetik, farmakodinamik, neoplazma, fareler, hayvan modelleri, histopatoloji

Effects of cysteamine on 3-methylcholanthrene - induced fibrosarcoma in mice

Understanding the biology, developing methods for prevention and treatment of tumors is of great importance. The present study was investigated protective effects of the cysteamine on 3-methylcholantrene-induced fibrosarcoma in mice. In the experiment, white male mice (Mus musculus albino) were used approximately 20±2.0 g in weight. Divided into five groups, per group of 15 mice and were fed ad libitum with a standard diet and water. No drug was performed in the first group of animals. Sesame oil (0.2 ml volume) for the second group of mice were injected subcutaneously. The third group was given in drinking water 0.1% solution of cysteamine (ad libitum). 3-methylcholantrene solution (1 mg/0.2ml sesame oil) were injected for the fourth group of mice subcutaneously with 0.2 ml volume. The five group was performed 0.2 ml volume of 3-methylcholantrene solution (1 mg/0.2 ml sesame oil) subcutaneously and 0.1% dissolved in water was ad libitum disintegrating orally. Animals were observed for 4 months. The mice that euthanasiaded cervical dislocation were autopsied at the end. Morphological changes in organs and tissue samples taken from tumor formation was investigated with histopathological methods. In conclusion ıt is suggested that cysteamine has a protective effect on 3-methyl-cholantrene-induced fibrosarcoma in mice.

Keywords:

cysteamine, laboratory animals, tissue distribution, sarcoma, pharmacology, pharmacokinetics, pharmacodynamics, neoplasms, mice, animal models, histopathology,

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