SERPİL DAĞ, DOĞAN AKÇA, MUSA KARAMAN, ÖZGÜR ÇELEBİ, HASAN ÖZEN, FATİH BÜYÜK, MİTAT ŞAHİN

Use of immunoperoxidase technique in smears prepared from vaginal secretions in early diagnosis of listerial abortions in cattle

Listeriozis; ruminantlarda meningoensefalitis, septisemi ve abortusa neden olan, genellikle sporadik seyirli bir enfeksiyondur. Bu çalışmada sığır listerial abortlarında erken ön tanı amacıyla immunoperoksidaz boyama yönteminin kullanılabilirliği araştırıldı. Bu amaçla atık yapan 96 sığırdan alınan vajinal sıvap örneklerinden hazırlanan sürme preparatlar L. monocytogenes antikoru ile immunoperoksidaz boyama yöntemi kullanılarak boyandı. Bakteriyolojik kültür metodu ile vajinal sıvap örneklerinde etkenin varlığı araştırılarak immunoperoksidaz yönteminin sonuçları ile karşılaştırıldı. Bakteriyolojik kültürü pozitif olan 5 adet vajinal sıvap örneğinden hazırlanan sürme preparatların 4ünde ve negatif sonuçlanan 91 adet örneğin 3ünde olmak üzere toplam 7 örnekte immunoperoksidaz ile pozitif reaksiyon saptandı. İmmunoperoksidaz metodu kültür metodu ile karşılaştırıldığında duyarlılığı ve özgüllüğü sırasıyla %80 ve %96.7 olarak saptandı. Sonuç olarak vajinal akıntılardan hazırlanan sürme preparatlarda immunoperoksidaz tekniğinin aynı gün içerisinde sonuç alınması nedeni ile listerial abortların ön tanısı amacıyla kullanılabileceği ancak bu yöntemin etken izolasyon ve identifikasyonuna yönelik olarak yürütülen bakteriyolojik kültür yöntemi ile desteklenmesi gerektiği sonucuna varıldı.

Anahtar Kelimeler:

immunoperoksidaz tekniği, sığır, Listeria monocytogenes, yavru atma, doğruluk, bakteriyel hastalıklar, teşhis, tanı teknikleri, üreme bozuklukları, vajina smiri

Sığır listerial abortlarının ön tanısında vajinal akıntılardan hazırlanan sürme preparatlarda immunoperoksidaz yönteminin kullanılması

Listeriosis is a sporadic disease of ruminants that causes meningoencephalitis, septicemia and abortion. In this study, the usefulness of immunoperoxidase technique was investigated in early diagnosis of cattle listerial abortions. For this purpose, 96 smears prepared from vaginal swab samples that were collected from aborting cattle were stained for L. monocytogenes by immunoperoxidase technique. Presence of the agent in vaginal swab samples were investigated by bacteriological culture technique and the results were compared to that of immunoperoxidase technique. A total of 7 samples, 4 out 5 bacteriological culture positive smear samples and 3 out of 91 bacteriological culture negative smear samples, were detected to be positive by immunoperoxidase technique. Compared to bacteriological culture technique, sensitivity and specificity of immunoperoxidase technique was calculated as 80% and 96.7%, respectively. In conclusion, immunoperoxidase technique in smears prepared from vaginal swabs can be used in early diagnosis of listerial abortions since it can give results the same day samples collected, however the technique must be supported by bacteriological culture technique which is performed for bacterial isolation and identification of the agent.

Keywords:

immunoperoxidase technique, cattle, Listeria monocytogenes, abortion, accuracy, bacterial diseases, diagnosis, diagnostic techniques, reproductive disorders, vaginal smears,

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