FARUK BOZKAYA, ŞÜKRÜ GÜRLER, Mugdat YERTÜRK

Genetic variability of CSN1S1 gene in goat populations raised in southeastern region of Turkey

Bu çalışmanın amacı güneydoğu anadolu bölgesinde yetiştirilen keçilerde alfa-s1-kazeini kodlayan CSN1S1 genindeki çeşitliliğin araştırılmasıdır. Türkiye’nin Kilis (n=60) Şanlıurfa (n=56) ve Siirt (n=55) illerinde yetiştirilen keçilerden kan örnekleri toplanmıştır. Kan örneklerinden fenol-kloroform yöntemi ile DNA izolasyonu yapılmıştır. Keçilerin genotipleri polimeraz zincir reaksiyonu (PCR), allel spesifik PCR ve kesim bölgesi polimorfizmi yöntemleri ile belirlenmiştir. Kilis ve Şanlıurfa populasyonlarında A*, B*, F ve N allelleri gözlenirken Siirt populasyonunda sadece A* ve B* allelleri bulunmuştur. A*, B*, F and N allellerinin frekansları sırasıyla Kilis keçilerinde 0.375, 0.367, 0.017 ve 0.242, Şanlıurfa populasyonunda 0.632, 0.208, 0.009 ve 0.151, Siirt populasyonunda ise 0.782, 0.218, 0.000 ve 0.000, rolarak hesaplanmıştır. İncelenen populasyonlarda CSN1S1 E ve 01 allelleri gözlenmemiştir. Beklenen ve gözlenen genotip frekansları arasında önemli bir farklılık bulunmamıştır (P>0.05). Çalışma sonucunda özellikle Kilis ve Şanlıurfa populasyonlarında değişik yetiştirme hedefleri açısından seleksiyon yapılabilecek düzeyde genetik çeşitliliğin bulunduğu kanaatine varılmıştır.

Anahtar Kelimeler:

seleksiyon, Türkiye, alleller, hayvan ıslahı, ırk farklılıkları, genler, genetik çeşitlilik, genotip, keçi ırkları, keçi, süt hayvanları, populasyon genetiği

Güneydoğu anadolu bölgesi’nde yetiştirilen keçilerde alfa-s1-kazein (CSN1S1) genindeki genetik çeşitlilik

The objective of this study was to investigate genetic variability of CSN1S1 gene coding for alpha-s1-casein in goat populations raised in Southeastern Region of Turkey. Blood samples were collected from goats raised in Kilis(n=60), Sanliurfa (n=56), and Siirt (n=55) provinces of Turkey. From the blood samples DNA was isolated by using phenol-chloroform extraction. Genotypes of animals were determined by using polymerase chain reaction (PCR), allele specific PCR or PCR and restriction fragment length polymorphism methods. In Kilis and Sanliurfa populations CSN1S1 A*, B*, F and N alleles were observed, while in Siirt population only A* and B* alleles were found. Frequencies of A*, B*, F and N alleles were 0.375, 0.367, 0.017 and 0.242 in Kilis, 0.632, 0.208, 0.009 and 0.151in Sanliurfa and 0.782, 0.218, 0.000 and 0.000 in Siirt populations, respectively. CSN1S1 E and 01 alleles were not observed among the populations studied. Observed and expected genotype frequencies did not differ significantly (P>0.05). The results of this study suggested that there were sufficient genetic variability of CSN1S1 gene especially in Sanliurfa and Kilis populations in order to select individuals for different breeding purposes.

Keywords:

selection, Turkey, alleles, animal breeding, breed differences, genes, genetic variation, genotypes, goat breeds, goats, milk yielding animals, population genetics,

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