Özgür ÇELEBİ, Fatih BÜYÜK, Doğan AKÇA, Elif ÇELİK, Mustafa Reha COŞKUN, Mitat ŞAHİN, Aliye GÜLMEZ SAĞLAM, Salih OTLU

Abort Öykülü Koyun Sürülerinden Alınan Vaginal Sıvap Örneklerinden Campylobacter spp. İzolasyonu ve Moleküler İdentifikasyonu

Campylobacteriosis, sığır ve koyunlarda yavru atımı ve infertilite ile karakterize, bulaşıcı ve zoonotik bir infeksiyondur. Bu çalışmada, Kars ilindeki koyun sürülerinde gözlenen abort olaylarının Campylobacter spp. yönünden araştırılması amaçlandı. Bu amaçla, abort olgularının görüldüğü koyun sürülerinden alınan toplam 350 adet vaginal sıvap örneği kültürel ve moleküler yöntemlerle incelendi. Campylobacter türlerinin izolasyonu amacıyla alınan örneklerin Preston Campylobacter Selektif Agara ekimleri yapıldı. Üreme sonucu şüpheli kolonilere identifikasyon amacıyla, Gram boyama ve hareketlilik muayeneleri ile oksidaz ve katalaz testleri uygulandı. Campylobacter spp. olarak belirlenen şüpheli kolonilerin tür düzeyinde identifikasyonu için Multiplex PCR (m-PCR) kullanıldı. Toplamda incelenen 350 vaginal sıvap örneğinin 8 (%2.28)’inde Campylobacter spp. izolasyonu gerçekleştirildi. Multiplex PCR sonucunda 3’ü Campylobacter jejuni ve 5’i C. coli olarak tespit edildi. Bu çalışma sonucunda elde edilen verilere bakılarak yöremizdeki koyunlarda meydana gelen atık olgularında Campylobacter türlerinin de göz önüne alınması gerektiği ortaya konulmuştur. Bu infeksiyonun hem hayvan sağlığı hem de insan sağlığı açısından oluşturduğu risk göz önüne alınırsa koruma ve kontrol tedbirleri açısından daha fazla önem verilmesi gerektiği düşünülmektedir.

Isolation and Molecular Identification of Campylobacter spp. from Vaginal Swab Sample Obtained from Sheep Herds with Abort History

Campylobacteriosis is a contagious and zoonotic infection characterized by abortion and infertility in cattle and sheep. The objective of thisstudy was to investigate Campylobacter spp. cause to abortion in sheep herds in Kars province. For this purpose, a total of 350 vaginal swabsamples obtained from sheep with abortion were examined by cultural and molecular methods. Swab samples were inoculated on PrestonCampylobacter Selective Agar for isolation of Campylobacter species. Following the incubation, suspected colonies were subjected to Gramstaining, mobility, oxidase and catalase tests for identification. Multiplex PCR (m-PCR) was used for the identification of Campylobacterisolates at species level. Campylobacter spp. was isolated in 8 (2.28%) of the 350 vaginal swab samples examined. Of 3 isolates were identifiedas Campylobacter jejuni and 5 were C. coli by m-PCR. According to the data obtained from this study, it was revealed that Campylobacterspecies should be taken into consideration in the abortion cases of sheep in this region. Considering the risk of this infection both in terms ofanimal health and human health, it is thought that more attention should be given to protection and control measures.

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