Hong YIN, Ji-Jie LIU, Dan YANG, Hui-Qing XU

Effect of β-Casomorphin-7 on Intestinal Mucosal Immunity in Aged Mice

The immune deficiency caused by aging deserves attention, especially the weakening of intestinal mucosal immunity. The effect of β-casomorphin-7 on intestinal mucosal immunity was investigated in aged mice. Mice were treated without or with different doses of β-casomorphin-7 for 30 days. Histopathological studies showed the tissue protective role of β-casomorphin-7 in aged mice. Lowdose group could significantly increase the level of IL-2 and TNF-α in intestinal mucosa. A significant increase in the level of SIgA was observed in medium- and high-dose groups. The low and medium dose groups could significantly increase the activity of SOD in small intestine mucosa. All dose groups significantly reduced the levels of MDA. The results suggest that β-casomorphin-7 could improve intestinal mucosal immune decline which is induced by aging The mechanisms for the regulating effects were likely through balancing the cytokine level and controlling the oxidative stress.

Yaşlı Farelerde Bağırsak Mukozası Bağışıklığına β-Kazomorfin-7’nin Etkisi

Yaşlanmaya bağlı bağışıklık yetersizliği özellikle bağırsak mukozası bağışıklığı olmak üzere dikkat edilmesi gereken bir husustur. Bu çalışmada, yaşlı farelerin bağırsak mukozası bağışıklığına β-kazomorfin-7’nin etkisi araştırılmıştır. Farelere 30 gün süresince farklı dozlarda β-kazomorfin-7 içeren veya içermeyen uygulamalar yapıldı. Histopatolojik incelemelerde β-kazomorfin-7’nin yaşlı farelerde doku koruyucu etkisinin olduğu gözlemlendi. Düşük doz grubunda bağırsak mukozasında IL-2 ve TNF-α seviyeleri anlamlı derecede arttı. Orta ve yüksek doz gruplarında SIgA seviyesinde anlamlı bir artma gözlemlendi. Düşük ve orta doz gruplarında ince bağırsak mukozasında SOD aktivitesi anlamlı derecede arttı. Tüm doz gruplarında, MDA aktivitesi anlamlı derecede azaldı. Elde edilen sonuçlar, β-kazomorfin-7’nin yaşlılığa bağlı olarak gelişen bağırsak mukozası bağışıklığında meydana gelen düşüşü iyileştirebileceğini gösterdi. Bu düzenleyici etkiyi muhtemelen sitokin seviyesi ve oksidatif stresi kontrol altında tutmak suretiyle oluşturmaktadır.

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