The Effects of Oxytocin and PGF2α Injections on Semen Quality and Libido in Buck

The aim of the present study was to evaluate the effects of exogenous oxytocin and PGF2α on seminal quality and libido sexualis in bucks. To investigate the role of these hormones on male fertility, semen samples from 20 Norduz bucks (3-4 years of age) were collected with an artificial vagina twice a week with five replications in breeding season. Bucks were randomly assigned to five groups, control group was administered with 2 mL of sodium chloride, 0.9% (w/v)) i.m., whilst the experimental groups were administered with oxytocin 10 IU, i.v. (Group 1, n = 5), oxytocin 20 IU, i.v. (Group 2, n = 5), PGF2α 5 mg, i.m. (Group 3, n = 5) or PGF2α 10 mg, i.m. (Group 4, n = 5) 20 min before each collection. There was no statistical difference between the treatment groups in terms of motility (P>0.05). However, semen volume, concentration, abnormal spermatozoa rate, intact membrane rate and libido results were statistically significant among the different groups (P

Tekelerde Oksitosin ve PGF2α Enjeksiyonlarının Sperma Kalitesi ve Libido Üzerine Etkisi

Bu çalışmanın amacı, ekzojen yoldan uygulanan oksitosin ve PGF2α’nın seminal plasma ve libido seksüalis üzerine olan etkilerini değerlendirmekti. Bu hormonların sezon içi erkek reprodüksiyonu üzerindeki rolünü araştırmak için, 20 Norduz tekesinden (3-4 yaş) haftada iki kez suni vajina ile alınan sperma numuneleri, beş replikasyon ile gerçekleştirildi. Tekeler rastgele beş gruba ayrılarak, sperm alma işleminden 20 dk önce, kontrol grubu 2 mL sodyum klorür, %0.9 (w/v)) ile i.m., deney grupları ise oksitosin 10 IU, i.v. (Grup 1, n = 5), oksitosin 20 IU, i.v. (Grup 2, n = 5), PGF2α 5 mg, i.m. (Grup 3, n = 5) ve PGF2α 10 mg, i.m. (Grup 4, n = 5) olarak uygulandı. Deney grupları arasında motilite açısından istatistiksel fark tespit edilmedi (P>0.05). Ancak sperma hacmi, konsantrasyon, anormal spermatozoa oranı, intakt membran oranı ve libido sonuçları gruplar arasında istatistiksel olarak anlamlı bulundu (P

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1. Paramio MT, Izquierdo D: Assisted reproduction technologies in goats. Small Ruminant Res, 121 (1): 21-26, 2014. DOI: 10.1016/j.smallrumres. 2014.01.002

2. Singh S, Bhakat M, Mohanty TK, Kumar A, Gupta AK, Chakravarty AK, Singh P: Sexual behavior and its relationship with semen quality parameters in Sahiwal breeding bulls. Vet World, 8 (6): 745-749, 2015. DOI: 10.14202/vetworld.2015.745-749

3. Savic R, Petrovic M: Variability in ejaculation rate and libido of boars during reproductive exploitation. S Afr J Anim Sci, 45 (4): 355-361, 2015. DOI: 10.4314/sajas.v45i4.1

4. Tirpan MB, Gürler H, Olğaç, KT, Daşkın A: Effects of boron added bull semen extender on post-thaw spermatological parameters. Ankara Univ Vet Fak Derg, 65 (2): 123-128, 2018.

5. Monaco D, Fatnassi M, Padalino B, Aubé L, Khorchani T, Hammadi M, Lacalandra GM: Effects of a GnRH administration on testosterone profile, libido and semen parameters of dromedary camel bulls. Res Vet Sci, 102, 212-216. 2015. DOI: 10.1016/j.rvsc.2015.08.011

6. Kumar BSB, Pandita S, Prakash BS, Mallick S, Mohanty TK, Mandal DK, Mili B: Luteinizing hormone, testosterone and total estrogens response to exogenous GnRH in crossbred bulls with differing semen quality. Livest Sci, 174, 150-153, 2015. DOI: 10.1016/j.livsci.2015.01.019

7. Patel DP, Chandrapal JC, Hotaling JM: Hormone-based treatments in subfertile males. Curr Urol Rep, 17 (8): 56, 2016. DOI: 10.1007/s11934- 016-0612-4

8. Mylonas CC, Duncan NJ, Asturiano JF: Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality. Aquaculture, 472, 21-44, 2017. DOI: 10.1016/j.aquaculture. 2016.04.021

9. Yıldız S, Güngör Ö, Tuncer PB, Tașdemir U, Erol H, Kacar C, Bucak MN: The effects of deslorelin, GnRH analogue with long acted on sexual activities in Angora bucks. Kafkas Univ Vet Fak Derg, 15 (1): 95-101, 2009. DOI: 10.9775/kvfd.2008.83-A

10. Gruson D: Oxytocin testing and reproductive health: Status and clinical applications. Clin Biochem, 62, 55-61, 2018. DOI: 10.1016/j. clinbiochem.2018.10.016

11. Veening JG, De Jong TR, Waldinger MD, Korte SM, Olivier B: The role of oxytocin in male and female reproductive behavior. Eur J Pharmacol, 753, 209-228, 2015. DOI: 10.1016/j.ejphar.2014.07.045

12. Stelletta C, Tekin K, Tırpan MB, Alemdar H, Çil B, Öztutar Stelletta F, Olgaç KT, İnanç ME, Daşkın A: Vulvar thermal pattern following synchronization of estrus is linked to fertility after timed artificial insemination in goat. Theriogenology, 103, 137-142. 2017. DOI: 10.1016/j. theriogenology.2017.07.038

13. Tırpan MB, Tekin K, Çil B, Alemdar H, İnanc ME, Olgaç KT, Stelletta C, Daşkın A: The effects of different PMSG doses on estrus behavior and pregnancy rate in Angora goats. Animal, 13 (3): 564-569, 2019. DOI: 10.1017/S1751731118001908

14. Saeed SA, Anwar N, Khan KM, Sarfraz N: Effect of chronic treatment with a cyclooxygenase inhibitor on reproductive parameters in male rat. J Ayub Med Coll, 21 (3): 66-71, 2009.

15. Rossitto M, Ujjan S, Poulat F, Boizet-Bonhoure B: Multiple roles of the prostaglandin D2 signalling pathway in reproduction. Reproduction, 149 (1): R49-R58. 2015. DOI: 10.1530/REP-14-0381

16. Ibrahim MA: Influence of oxytocin and prostaglandin on semen characteristics and process of ejaculation in buffalo bulls. Acta Vet Hung, 36, 3-10, 1988.

17. Hess MB: The effects of prostaglandin F2a, oxytocin and gonadotropin releasing hormone on ejaculate characteristics in the dog, MSc Thesis, Virginia Polytechnic Institute and State University, 2002.

18. Şen ÇÇ, Akcay E: The effect of oxytocin and prostaglandin hormones added to semen on stallion sperm quality. Turk J Vet Anim Sci, 39, 705-708, 2015. DOI: 10.3906/vet-1412-69

19. Palmer CW, Amundson SD, Brito LFC, Waldner CL, Barth AD: Use of oxytocin and cloprostenol to facilitate semen collection by electroejaculation or transrectal massage in bulls, Anim Reprod Sci, 80, 213-223, 2004. DOI: 10.1016/j.anireprosci.2003.07.003

20. Nicholson HD, Guldenaar SEF, Boer GJ, Pickering BT: Testicular oxytocin: Effects of intratesticular oxytocin in the rat. J Endocrinol, 130 (2): 231-238, 1991. DOI: 10.1677/joe.0.1300231

21. Berndtson WE, Igboeli G: Spermatogenesis, sperm output and seminal quality of Holstein bulls electroejaculated after administration of oxytocin. J Reprod Fertil, 82, 467-475, 1988. DOI: 10.1530/jrf.0.0820467

22. Nicholson HD, Parkinson TJ, Lapwood KR: Effects of oxytocin and vasopressin on sperm transport from the cauda epididymis in sheep. J Reprod Fertil, 117, 299-305, 1999. DOI: 10.1530/jrf.0.1170299

23. Fjellström D, Kihlström JE, Melin P: The effect of synthetic oxytocin upon seminal characteristics and sexual behaviour in male rabbits. J Reprod Fertil, 17, 207-209, 1968. DOI: 10.1530/jrf.0.0170207

24. Filippi S, Vannelli GB, Granchi S, Luconi M, Crescioli, C, Mancina R, Natali A, Brocchi S, Vignozzi L, Bencini E: Identification, localization and functional activity of oxytocin receptors in epididymis. Mol Cell Endocrinol, 193, 89-100, 2002. DOI: 10.1016/S0303-7207(02)00101-6

25. Whittington K, Assinder S, Gould M, Nicholson H: Oxytocin, oxytocin associated neurophysin and the oxytocin receptor in human prostate. Cell Tissue Res, 318, 375–382, 2004. DOI: 10.1007/s00441-004- 0968-5

26. Tırpan MB, Tekin N: Effects of boron (sodium pentaborate), added instead of Tris components, on freezing and post-thaw quality of Angora buck semen. Ankara Univ Vet Fak Derg, 62, 295-302, 2015.

27. Kerketta S, Singh M, Patel BHM, Verma M, Prasad JK, Upadhyay D, Bhushan B: Study on buck’s mating behaviour, libido score and semen biology in local goat of Rohilkhand region, India. Indian J Anim Sci, 48, 491-495, 2015. DOI: 10.5958/0976-0555.2014.00017.X

28. Revell SG, Mrode RA: An osmotic resistance test for bovine semen. Anim Reprod Sci, 36, 77-86, 1994. DOI: 10.1016/0378-4320(94)90055-8

29. Traas AM, Kustritz MVR: Effect of administrating oxytocin or prostaglandin F2α on characteristics of the canine ejaculate. Can Vet J, 45, 999-1002, 2004.

30. Veronesi MC, De Amicis I, Panzani S, Kindahl H, Govoni N, Probo M, Carluccio A: PGF2α, LH, testosterone, oestrone sulphate, and cortisol plasma concentrations around sexual stimulation in jackass. Theriogenology, 75, 1489-1498, 2011. DOI: 10.1016/j.theriogenology. 2010.12.010

31. Honda K, Yanagimoto M, Negoro H, Narita K, Murata T, Higuchi T: Excitation of oxytocin cells in the hypothalamic supraoptic nucleus by electrical stimulation of the dorsal penile nerve and tactile stimulation of the penis in the rat. Brain Res Bull, 48 (3): 309-313, 1999. DOI: 10.1016/ S0361-9230(98)00180-4

32. Thackare H, Nicholson HD, Whittington K: Oxytocin its role in male reproduction and new potential therapeutic uses. Hum Reprod Update, 12, 437-448, 2006. DOI: 10.1093/humupd/dmk002

33. Carter CS: Oxytocin and sexual behavior. Neurosci Biobehav Rev, 16, 131-144, 1992. DOI: 10.1016/S0149-7634(05)80176-9

34. Walch K, Eder R, Schindler A, Feichtinger W: The effect of singledose oxytocin application on time to ejaculation and seminal parameters in men. J Assist Reprod Genet, 18, 655-659, 2001. DOI: 10.1023/ A:1013115301159

35. Lui C, Cui XG, Wang YX, You, ZD, Xu DF: Association between neuropeptide oxytocin and male infertility. J Assist Reprod Genet, 27, 525- 531, 2010. DOI: 10.1007/s10815-010-9451-2

36. Stoneham MD, Everitt BJ, Hansen S, Lightman SL, Todd K: Oxytocin and sexual behavior in the male rat and rabbit. J Endocrinol, 107, 97-106, 1985. DOI: 10.1677/joe.0.1070097

37. Arletti R, Bazzani C, Castelli M, Bertolini A: Oxytocin improves male copulatory performance in rats. Horm Behav, 19, 14-20, 1985. DOI: 10.1016/0018-506X(85)90002-9

38. Alcay S, Toker B, Ustuner B, Nur Z, Sagirkaya H, Soylu MK: Investigation of relationships between DNA integrity and fresh semen parameters in rams. Kafkas Univ Vet Fak Derg, 20 (5): 793-798. 2014. DOI: 10.9775/kvfd.2014.11144

39. Knight TW, Lindsay DR: Short-and long-term effects of oxytocin on quality and quantity of semen from rams. J Reprod Fertil, 21, 523-529, 1970.

40. Çiftçi HB: In-vitro effect of oxytocin on the duration of sperm motility and morphology. J Anim Vet Adv, 4, 794-797, 2005.

41. Nicholson HD, Warley RTS, Charlton HM, Pickering BT: LH and testosterone cause the development of seminiferous tubule contractile activity and the appearance of testicular oxytocin in hypogonadal mice. J Endocrinol,110, 159-167, 1986.

42. Nicholson HD, Warley RTS, Guldenaar SEF, Pickering BT: Ethan- 1,2-dimethanesulphonate reduces testicular oxytocin content and seminiferous tubule movements in the rat. J Endocrinol, 112, 311-316, 1987.

43. Roser JF: Endocrine and paracrine control of sperm production in stallions. Anim Reprod Sci, 68, 139-151, 2001. DOI: 10.1016/S0378- 4320(01)00151-8

44. Bygdeman M: Prostaglandins. In, Hodgson JE (Ed): Abortion and Sterilization: Medical and Social Aspects. 333-358, Academic Press, London, 1981. DOI: 10.1016/B978-0-12-792030-6.50019-0

45.Hashit ani H, Yanai Y, Shirasawa N, Soji T, Tomita A, Kohri K, Suzuki H: Interaction between spontaneous and neurally mediated regulation of smooth muscle tone in the rabbit corpus cavernosum. J Physiol, 569, 723-735, 2005. DOI: 10.1113/jphysiol.2005.099309

46. Veronesi MC, Tosi U, Villani MARTA, Govoni N, Faustini M, Kindahl H, Madej A, Carluccio A: Oxytocin, vasopressin, prostaglandin F2α, luteinizing hormone, testosterone, estrone sulfate, and cortisol plasma concentrations after sexual stimulation in stallions. Theriogenology, 73, 460-467, 2010. DOI: 10.1016/j.theriogenology.2009.09.028

47. Hemsworth PH, Donnelly J, Findlay JK, Galloway DB: The effects of prostaglandin F2α on sperm output in boars. Prostaglandins, 13, 933-941, 1977. DOI: 10.1016/0090-6980(77)90223-4
Kafkas Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 1300-6045
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1995
  • Yayıncı: Kafkas Üniv. Veteriner Fak.
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