ZEKİ ARAS, MEHMET TAŞPINAR, İBRAHİM AYDIN

A novel polymerase chain reaction to detect brucella canis in dogs

Bu çalışmada, köpeklerin vajinal sıvap örneklerinden Brucella canis (B. canis)in direk teşhisi amacıyla spesifik polimeraz zincir reaksiyonu metodu standardize edildi ve değerlendirildi. Brucella spp.in 16S-23S inter-space bölgesine ve B. canisin BMEI1426-BMEI1427 gen bölgesinde ki 351 bplik delesyon alanına özgü primer çiftleri seçildi. polimeraz zincir reaksiyonu yönteminin değerlendirilmesinde toplam 21 referans ve saha suşu ile 35 vajinal sıvap örneği kullanıldı. Sadece 214 bplik amplifiye polimeraz zincir reaksiyonu ürününün görüntülenmesi B. canis için pozitif olarak kabul edildi. Vajinal sıvap örneklerinden en az DNA tespit limiti yeni metot için 2.7 x 10 1 CFU/g bakteri olarak bulundu. Bu yöntem bakteriyel izolasyon metoduna pratik bir alternatif olabilir. Yeni polimeraz zincir reaksiyonu testi B. canisin klinik veya saha örneklerinden 24 saat sürede ve tek adımda hızlı ve basit tespitini sağlamaktadır.

Köpeklerde brucella canisin teşhisi amacıyla yeni polimeraz zincir reaksiyonu metodunun geliştirilmesi

In this study, the specific polymerase chain reaction has been standardized and evaluated for the direct diagnosis of Brucella canis in vaginal swab samples from dogs. The specific primer sets are directed to the 16S-23S rRNA inter-space region of Brucella spp. and the deletion of 351 bp in BMEI1426-BMEI1427 in B. canis. A total of 21 references and field strains and 35 vaginal swab samples were used for the evaluation of the polymerase chain reaction. It found that polymerase chain reaction is positive for B. canis DNA indicated by only ampliﬁcation of 214 bp product. It detected at least 2.7 x 10 1 CFU/g of bacteria diluted in vaginal swab samples indicates that the polymerase chain reaction can be used as a practical alternative for bacterial isolation. The novel polymerase chain reaction provides a simple and rapid for the detection of B. canis in clinical and field samples in one step and in short time about 24 h.

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