Hypolipidemic effects of Satureja Khuzistanica essential oil in broiler chicken are realized through alteration in steroid hormones

Yüksek ve düşük doz Satureja Khuzistanica esansiyel yağ (SEO) uygulamasının broyler civcivlerde kan lipid bileşenleri ve seks hormonları üzerine etkilerini değerlendirmek üzere iki çalışma yürütüldü. Birinci çalışmada, 420 adet her iki cinsiyetten bir günlük Cobb 500 civciv rastgele olarak her birinde 20 adet civciv bulunan 21 adet yer kümeslerine yerleştirildi ve 3 tekrar olmak üzere içme suyu içerisinde 0, 0.5, 1.0, 1.5, 2.0 ve 2.5 g/L SEO veya 3.0 g/L Polysorbate-80 (kontrol+) uygulamasının etkileri incelendi. İkinci çalışmada, 720 adet yumurtadan yeni çıkmış erkek Arian civciv rastgele olarak her birinde 20 adet civciv bulunan 36 adet yer kümeslerine yerleştirildi ve 6 tekrar olmak üzere 0, 0.2, 0.3, 0.4 ve 0.5, g/L SEO veya 0.5 g/L Polysorbate-80 (kontrol+) uygulamasının etkileri incelendi. Çalışma sonucunda 1 çalışmada 0.5 ve 2. Çalışmada 0.3 g/L SEO uygulamasının serum kolesterol ve yüksek yoğunluklu lipoprotein (HDL) seviyelerini azalttığı ancak farkların kontrol grupları ile karşılaştırıldığında anlamlı olmadığı (P>0.05) tespit edilmiştir. 0.5 g/L SEO 28. günde civcivlerin abdominal yağ yüzdesinde (%16) anlamlı bir düşmeye neden oldu (Deney 1; P0 .05). Elde edilen sonuçlar broyler tavuklarda SEO için testosteron ilişkili hipolipidemik etkilerin olabileceğini göstermektedir.

Broyler Tavuklarda Satureja khuzistanica esansiyel yağın hipolipidemik etkileri steroid hormonlardaki değişim yoluyla oluşturulur

Two experiments were conducted to evaluate the efects of high and low doses of Satureja Khuzistanica essential oil (SEO) on blood lipid constituents and sex hormones in broiler chicks. In trail 1, 420 one-day-old Cobb 500 chicks of both sexes were randomly placed in 21 foor pens to examine the efect of supplementation of drinking water with 0, 0.5, 1.0, 1.5, 2.0, and 2.5 g/L SEO or 3.0 g/L Polysorbate-80 (control+) in 3 replicates of 20 birds each. In Experiment 2, 720 newly hatched male Arian chicks were randomly assigned to 36 foor pens to examine the efect of 0, 0.2, 0.3, 0.4 and 0.5, g/L SEO or 0.5 g/L Polysorbate-80 (control+) in 6 replicates of 20 birds each. As a result of this study, SEO at 0.5 and 0.3 g/L in Experiments 1 and 2, respectively, modulated serum cholesterol and high density lipoproteins (HDL) levels, albeit the diferences were not significant when compared to appropriate control groups (P>0.05). The SEO at 0.5 g/L caused significant decrease (16%) in abdominal fat percentage of the birds at 28 d (Trial 1; P<0.05). In trail 2, inclusion of SEO into drinking water at 0.2, 0.3, 0.4 and 0.5 g/L significantly reduced the serum estradiol to 50, 50 and 45 percent, respectively, and increased serum testosterone level by about 2, 4, 4 and 5 folds, respectively, in comparison with the control- birds (P>0.05). The results propose the possibility of testosterone-coupled hypolipidemic properti es for SEO in broiler chicken.

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1. Agricultural Research Service (ARS): Dr. Dukes phytochemical and ethnobotanical databases. Accessed at June, 2002. http://www.ars-grin. gov/duke , 2002.
2. Cuppett SL and Hall CA: Antioxidant activity of Labiatae. Adv Food Nutr Res, 42, 245-271, 1998. DOI : 10.1016/S1043-4526(08)60097-2
3. Burt S: Essential oils: Their antibacterial properties and potential applications in food-A review. Int J Food Microbiol, 94, 223-253, 2004. DOI : 10.1016/j.ijfoodmicro.2004.03.022
4. Abdollahi M, Salehnia A, Mortazavi SH, Ebrahimi M, Shafiee A, Fouladian F: Antioxidant, antidiabetic, antihyperlipidemic, reproduction stimulatory prop erties and safety of essential oil of Satureja khuzestanica in rat in vivo : A oxicopharmacological study. Med Sci Monit, 9, 331- 335, 2003.
5. Radonic A, Milos M: Chemical composition and in vitro evaluation of antioxidant efect of free volatile compounds from Satureja montana L. Free Radic Res, 37, 673-679, 2003.
6. Yamasaki K, Nakano M, Kawahata T, Mori H, Otake T, Ueba N, Oishi I, Inami R, Yamane M, Nakamura M, Murata H, Nakanishi T: Anti-HIV-1 activity of herbs in Labiatae. Biol Pharm Bull, 21, 829-833, 1998.
7. Azaz D, Demirci F, Satil F, Kurkcuoglu M, Baser KH: Antimicrobial activity of some Satureja essential oils. Z Naturforsch, 57, 817-821, 2002.
8. Skocibusic M, Bezic N: Phytochemical analysis and in vitro anti- microbial activity of two Satureja species essential oils. Phytother Res, 18, 967-970. 2004.
9. Sklan D, Berner YN, Rabinowitch HD: The efect of dietary onion and garlic on hepatic lipid concentration and activity of antioxidative enzymes in chicks. J Nutr Biochem , 3, 322-325, 1992. DOI : 10.1016/0955- 2863(92)90022-B
10. Konjufca VH, Pesti GM, Bakalli RI: Modulation of cholesterol levels in broiler meat by dietary garlic and copper. Poult Sci, 76, 1264- 12 71, 1997. DOI : 10.1093/ps/76.9.1264
11. Case GL, He L, Mo H, Elson CE: Induction of geranyle pyrophosphatase activity by cholesterol-suppressive isoprenoids. Lipids , 30, 357-359, 1995.
12. Lee KW, Everts H, Beynen AC: Essential oils in broiler nutrition. Int J Poult Sci, 3, 738-752, 2004.
13. Lee KW, Everts H, Kappert HJ, Frehner M, Wouterse H, Beynen AC: Cinnamanaldehyde, but not thymol, counteracts the carboxymethyl cellulose-induced growth depression in female broiler chickens. Int J Poult Sci, 3, 608-612, 2004.
14. Honda SF, Aoki F, Tanaka H, Kishida H, Nishiyama T, Okada S, Matsumoto I, Abe K, Mae T: Efects of ingested turmeric oleoresin on glucose and lipid metabolisems in obese diabetic mice: A DNA microarrey study. J Agric Food Chem, 54, 9055-9062, 2006. DOI: 10.1021/jf061788t
15. Sugiharto I, Widiastuti E, Prabowo NS: Efects of turmeric extract on blood parameters, feed efficiency and abdominal fat content in broilers. J Indonesian Trop Anim Agric, 36, 21-26, 2011.
16. Brenes A, Roura E: Essential oils in poultry nutrition: Main efects and modes of action. Anim Feed Sci Tech , 158, 1-14, 2010. DOI : 10.1016/j. anifeedsci.2010.03.007
17. Qureshi AA, Din ZZ, Abuirmeileh N, Burger WC, Ahmed Y, Elson CE: Suppression of avian hepatic lipid metabolisem by solvent extracts of garlic: Impact on serum lipids. J Nutr, 113, 1746-1755, 1983.
18. Elson CE, Qureshi AA: Coupling the cholesterol- and tumor- suppressive actions of palm oil to the impact of its minor constituents on 3-hydroxy-3-methylglutaryl coenzyme A reductase activity. Prostaglandins Leukot Essent Fatty Acids, 52, 205-208, 1995. DOI : 10.1016/0952- 3278(95)90024-1
19. Crowell PL: Prevention and therapy of cancer by dietary mono- terpenes. J Nutr, 129, 775S-778S, 1999.
20. Dehghani F, Azizi M, Panjehshahin MR: Toxic efects of hydro- alcoholic extract of Citrullus colocynthis on pregnant mice. Iranian J Vet Res, 9, 42-45, 2008.
21. Grigorova G, Kashamov B, Sredkova V, Surdjiiska S, Zlatev H: Efec t of tribulus terrestris extract on semen quality and serum tota cholesterol content in white plymouth rock-mini cocks. Biotech Anim Husband, 24, 139-146, 2008.
22. Hadian J, Mirjalili MH, Kanani MR, Salehnia A, Ganjipoor P: Phytochemical and morphological characterization of Satureja khuzistanica Jamzad populations from Iran. Chem Biodiver, 8, 902- 915, 2011. DOI 10.1002/cbdv.201000249.
23. Zargari A: Medicinal Plants. 4 th ed., 42-45, Tehran University Publications, Tehran, 1990.
24. Khosravinia H, Salehnia A, Rafiei Alavi E: Toxicity and safety assessment of Satureja khuzistanica essential oils in broiler chicks up to 28 days of age. Pp.178-179 in Proc. The 1 st National Medicinal Plants Congress 27-28 March, Iran, Sari. Univ. Mazandaran. 2010.
25. National Research Council (NRC): Nutrient Requirements of Poultry 9th ed., p.155, National Academy Press, Washington, DC., USA., ISBN 9780309048927, 1994.
26. SAS Institute: SAS/STAT® Guide for personal computers. Version 9.1 Edition. SAS Institute, Inc., Cary, NC, 2002.
27. Saxton AM: A macro for converting mean separation output to letter grouping in Proc Mixed. pp.1243-1264 in Proc. 23 rd SAS User Group Intl. SAS Institute, Cary, NC, 1998.
28. Saadoun A, Leclercq B: In vivo lipogenesis of genetically lean and fat chicken: Efects of nutritional state and dietary fat. J Nutr, 117, 428-435 1987.
29. Griffin HD, Guo K, Windsor D, Butterwith SC: Adipose tissue lipogenesis and fat deposition in leaner broiler chickens. J Nutr, 122, 363- 368, 1992.
30. Hermier D: Lipoprotein metabolism and fattening in poultry. J Nutr 127, 805S-808S, 1997.
31. Hotta M, Nakata R, Katsukawa M, Hori K, Takahashi S, Inoue H: Carvacrol, a component of thyme oil, activates PPAR and suppresses COX- 2 expression. J Lipid Res, 51, 132-139, 2010. DOI : 10.1194/jlr.M900255- JLR200
32. Can Baser KH: Biological and pharmacological activities of carvacro and carvacrol bearing essential oils. Curr Pharm Des, 14, 3106-3119, 2008 DOI: 10.2174/138161208786404227
33. Haeri S, Minaie B, Amin G, Nikfar S, Khorasani R, Esmaily H, Salehnia A, Abdollahi M: Efect of Satureja khuzistanica essential oil on male rat fertility. Fitoterapia , 77, 495-499, 2006.
34. Chen KL, Chi WT, Chiout PWS: Caponization and testosterone implantation efects on blood lipid and lipoproteins profile in male chicken. Poult Sci, 84, 547-552, 2005.
35. Middleton B, Hui KP: Inhibition of hepatic S-3-hydroxy-3-methyl- glutaryl-CoA reductase and in vivo rates of lipogenesis by a mixture of pure cyclic monoterpenes. Biochem Pharm, 31, 2897-2901, 1982.
36. Whitehead CC, Hood RL, Heard GC, Pym RAE: Comparison of plasma very low density lipoproteins and lipogenic enzymes as predictors of fat content and food conversion efficiency in selected lines of broiler chicken s. Br Poult Sci, 72, 1479-1491, 1 984.