Majid AHANGARAN GHOLAMI, Reza AZARGOON, Morteza Mohammad Hosseini ANARI

PCR Amplification of Helicobacter pullorum 16S rRNA gene in cecal content of pet birds

Helicobacter pullorum kaynağı olarak evcil kuşların değerlendirilmesi amacıyla İranda sistemik veya enterit klinik belirtileri göstermeyen 50 ispinoz ve 50 Avustralya papağanından toplam 100 sekum içeriği örneği toplandı. Sekal örneklerden DNA ekstraksiyonu yapıldı ve H. pullorum 16S rRNA geninin identifikasyonu amacıyla PCR gerçekleştirildi. H. pullorum geni 50 ispinozun 3ünde (%6) ve 50 Avustralya papağanının 6sında (%12) belirlendi. Çalışmanın sonuçları ispinoz ve Avustralya papağanının H. pullorum enfeksiyonu kaynağı ve bu etken için reservuar olabileceğini göstermiştir.

Evcil kuşların sekum İçeriklerinden Helicobacter pullorum 16S rRNA geninin amplifikasyonu

For evaluation of pet birds as a resource of Helicobacter pullorum, 100 cecal content samples were collected from 50 finches and 50 Australian parrots, with no clinical signs suggestive of systemic and/or enteric disease, in Iran. DNA was extracted from cecal samples and PCR was carry out for identification of H. pullorum 16S rRNA gene. Results showed, H. pullorum DNA was detected in 3 of the 50 (6%) cecal samples of finch and 6 out of 50 (12%) cecal samples of Australian parrot. This study represents that finch and Australian parrot can be a source of H. pullorum infection and serve as reservoirs for the H. pullorum.

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