RAPD (RASTGELE ARTTIRILMIŞ POLİMOFİK DNA) BELİRLEYİCİLERİ VE BİTKİ SİSTEMATİĞİ
RAPD (Rastgele Arttırılmış Polimofik DNA) genetik polimorfizmibelirleyen PCR temelli bir tekniktir. Tekniğin en büyük avantajları genomdizisi hakkında ön bilgiye, yüksek saflıktaki DNA’dan çok miktarlara,Southern blot veya radyoaktiv kimyasallara ihtiyaç duymamasıdır. Ayrıcahızlı ve düşük maliyetlidir. RAPD tekniği pek çok alanda olduğu gibi değişikbitki türlerinin genetik değişkenliğinin belirlenmesinde yaygın bir şekildebaşarıyla uygulanmaktadır.
RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) MARKERS AND PLANT SYSTEMATICS
RAPD (Randomly Amplified Polimorphic DNA)is a PCRbased method to detect genetic polymorphism. The major advantagesof RAPD include: no need for prior knowledge of genom sequence inpolymophism studies, large amount of DNA of high purity, Southernblot or radioactive chemicals. Furthermore, the technique is speed andlow cost. RAPD technique is being succesfully used widely for theestimation of genetic variability in various plant species.
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