Stage specificity in the expression of proteins of honey bee fat body (Apis mellifera L.) in the course of ontogenesis

Bu çalışmanın amacı ontogenez sırasında A. mellifera yağ dokusundaki protein ifadesinin yaşa bağlı özgünlüğündeki olağandışılıkların araştırılması ve analiz edilmesidir. %7.5 poliakrilamid jel elektroforezi kullanılarak, 189 yağ doku örneği bireylerin üçüncü, dördüncü ve beşinci larva zamanı (L3, L4, L5); pre-pupa (PP) ve beyaz-göz ve koyu-göz pupa (WP, DP) aşamaları ve yaşlarındaki gelişimleri süresince araştırılmıştır: Erkek ve dişi bireylerin yağ dokusunda gelişimleri boyunca toplam 25 protein örneği tanımlanmış ve bazı cinsiyet farklılıkları ortaya çıkmıştır. Yağ doku yelpazesinde bilinen proteinlerin tanımlanmasındaki yaş ve evreye özgü dinamikler analiz edilmiş ve yorumlanmıştır

Ontogenez sırasında bal arısı yağ dokusu proteinlerinin evreye özgü ekspresyonu

The aim of this study was to investigate and analyze some peculiarities in the age specificity of protein expression in the fat body of A. mellifera during the ontogenesis of the individuals. By using 7.5% ployacrylamide gel electrophoresis, 189 individual samples of fat body extracts were investigated in the course of their development in the following stages and ages: larvae-third, fourth and fifth larval age (L3, L4, L5); prepupae (PP) and pupae-white-eyed and dark-eyed pupae (WP, DP). A total of 25 protein fractions were expressed in the fat body of male and female individuals during their development. Some sex-differences in studied fat body proteins were noticed. The age and stage specific dynamics in the expression of established proteins of the fat body spectrum were analyzed and commented.

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