Chidambaram T. KUMARAPPAN, T.Nageswara RAO, Subhash C MANDAL

Polyphenolic extract of Ichnocarpus frutescens modifies hyperlipidemia status in diabetic rats

Bitkilerden elde edilen doğal ilaçlar diabetes mellitus tedavisi için etkili ve güvenli bir alternatif tedavi olarak düşünülmektedir. Halk hekimliğinde I. frutescens yaprakları kaynatılarak hazırlanan öz, diabetes mellitus semptomlarını azaltmak için kullanılır. Bu çalışma alloksan ile diyabetik olan sıçanlarda I. frutescens polifenolik ekstraktın (PPE) anti-diyabetik ve anti-hiperlipidemik etkisini değerlendirmek üzere planlanmıştır. Diyabet alloxan’un (150mg/kg vücut ağırlığı) bir kerede intraperitonal enjeksiyonu ile oluşturulmuştur. Alloksan diyabetik farelerde 150-300mg/kg vücut ağırlığı dozlarında kullanılan polifenolik ekstrakt, serum açlık kan şekeri seviyesinde önemli düşüş oluşturmuştur. PPE (21 gün boyunca 300mg/kg vücut ağırlığı) kullanımı hepatik HMG-CoA redüktaz aktivitesinde önemli düşüş oluşturmuştur. Normoglisemik sıçanlarda önemli bir etki bulunmamıştır. Hiperlipidemik indikatörlerin (TC, TG, VLDL,HDL, ve LDL) seviyelerindeki düzelmelerden de anlaşılacağı üzere polifenolik ekstrakt önemli hipolipidemik etki göstermiştir. Polifenolik ekstraktın (100mg/kg) oral kullanımı lipoprotein lipaz enzim salınımını önemli derecede hızlandırmıştır. Polifenolik ekstrakt ADP’nin neden olduğu trombosit kümelenmesini de önlemiştir. Bu sonuçlar, polifenolik ektstraktın alloksan diyabetik sıçanlarda hiperlipidemi ve obeziteye karşı etkinliğini göstermektedir. Polifenolik ekstrakt ile tedavi edilen alloksan sıçanlarının aort damarının histopatolijik incelemesi normal görünüme yakın bir düzelme olduğunu göstermiştir. Bütün sonuçlar, polifenolik ekstraktın diyabete, diyabetik komplikasyonlara ve kardiyovasküler riske neden olan hiperlipidemiye ve aterosikleroza karşı terapatik potansiyelini göstermiştir

Ichnocarpus frutescens polifenolik ekstraktı diyabetik sıçanlarda hiperlipidemik durumu etkiler

Natural remedies from medicinal plants are considered to be effective and safe alternative treatments for diabetes mellitus. Decoction prepared from the leaves of I. frutescens is used to alleviate the symptoms of diabetes mellitus in folk medicine. The present study was designed to evaluate the anti-diabetic and antihyperlipidemic effects of the polyphenolic extract (PPE) of I. frutescens leaves in alloxan induced diabetic rats. Diabetes was induced by single intraperitoneal injection of alloxan (150 mg/kg body weight). Polyphenolic extract to alloxan diabetic rats at the doses of 150 mg and 300 mg/kg body weight resulted in a significant reduction of fasting blood glucose (FBG) levels. PPE (300 mg/kg body weight for 21 days) administration showed significant decrease in hepatic HMG-CoA reductase activity of alloxan diabetic rats. No significant effects were found in the normoglycemic rats. Polyphenolic extract exhibited significant hypolipidemic effect as evident from correction of hyperlipidemic indicators (TC, TGs, VLDL, HDL and LDL). Oral administration of polyphenolic extract (100 mg/kg) significantly enhanced the release of lipoprotein lipase enzyme significantly. Polyphenolic extract also inhibited ADP-induced platelet aggregation in vitro. The results indicating the effectiveness of polyphenolic extract against hyperlipidemia and obesity in alloxandiabetic rats. The histopathological studies of aorta in polyphenolic extract treated alloxan-rats revealed almost recovery to normal appearance. All the results revealed the therapeutic potential of polyphenolic extract against diabetes, hyperlipidemia and atherosclerosis leading diabetic complications and cardiovascular risks.

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