Chaneasekar RAMAN, Suganthi L. MANOHAR, Nirmala XAVİER, Muthukalingan KRISHNAN

Expression of silk gene in response to P-soyatose (hydrolyzed soy bean protein) supplementation in the fifth instar male larvae of Bombyx mori

Bombyx mori L. ipek bezleri, kozaya dönüşmeden önceki dönemde fazla miktarda ipek proteinlerin üretiminin modellenmesinde kullanılmıştır. Sentezlenen ipek proteinlerinin miktarı, ipek larvaların beslenmesi geliştirilerek arttırılabilir. Bu çalışma B. mori erkek larvasının beşinci instar döneminde P-soyatoz (hidrolize edilmiş soya proteini) takviyesi ile ipek gen ekspresyonundaki değişimleri sunmaktadır. Soya protein takviyeli gruptaki ipek bezi proteinlerinin Western blot analizi, H ve L zincirleri için yoğun boyanma göstermiştir. P-soyatozla beslenmiş erkek larvada protein sentezi, kontrol grubuna göre artmıştır. Bu durum, Psoyatozla besili larvadaki arka ipek bezlerindeki 3H leucine işaretlemesi ile kendini göstermektedir. Northern blot analizi de ayrıca soya protein takviyesinin ipek fibroin sentezini, dolayısıyla fibroin mRNA seviyesini arttırdığını kanıtlamıştır. Bu sonuç, B.mori’nin ekonomik açıdan faydalı olacak özelliklerinin güçlendirilmesini yansıtmaktadır

Bombyx mori beşinci evre erkek larvasında P-soyatoz (hidrolize edilmiş soya proteini) takviyesine cevap olarak ipek geni ekspresyonu

The silk glands of Bombyx mori L. have been used as a model system in the production of large amount of silk proteins prior to cocoon spinning. The amount of silk proteins synthesized could be augmented by improving the nutritional status of silkworm larvae. The present study reports the changes in the silk gene expression by supplementing P-soyatose (hydrolyzed soy protein) during the fifth instar B. mori male larvae. Western blot analysis of the silk gland protein in the soy protein supplemented group demonstrated higher staining intensity of H and L chains. The rate of protein synthesis was increased in the male larva reared with P-soyatose in comparison to control group. This was indicated by high incorporation of 3H leucine in the posterior silk glands of supplemented larvae reared with P-soyatose. Northern blot analysis unequivocally prove that supplementation of soy protein increases the synthesis of silk fibroin, as it increases the levels of fibroin mRNA, which reflects fortification in the economic traits of B. mori.

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