Plant regeneration through somatic embryogenesis and polyamine levels in cultures of grasses of Thar Desert

Lasirus scindicus, Sorghum halepense ve Urochloa panicoide için potansiyel explant kaynağı olarak genç çiçeklerin bazal kısımları kullanılarak bitki rejenerasyonu için doku kültürü metotları geliştirilmiştir. 2,4- diklorofenoksiasetik asit’in (2-4,D) farklı konsantrasyonlarını içeren Murashige ve Skoog’s (MS) besi ortamları kullanılarak embriyonik kültürler başlatılmıştır. 26±2 oC’de ve karanlıkta MS besi ortamında alt kültürleme ile rejeneratif kültürler elde edilmiştir. Hormonsuz MS bazal besi yeri veya 1mgl-1kinetin’li MS besi yeri içeren kompetan hücre kültürlerinde somatik embriyogenez ile bitki rejenerasyonu gerçekleştirilmiştir. Somatik fideler başarılı bir şekilde saksılara ekilmiş ve arazide büyütülmüştür. Embriyonik ve embriyonik olmayan kültürlerde poliamin seviyeleri incelenmiştir. Somatik embriyogenez ile bitki rejenerasyonu özelliği olan kültür dokularında poliamin seviyesi ve putresin-spermidin oranı arasında bir korelasyon olduğu tespit edilmiştir.

Thar çölü otları kültürlerinde somatik embriyogenez ile bitki rejenerasyonu ve poliamin seviyeleri

Tissue culture methods for plant regeneration have been developed for Lasiurus scindicus, Sorghum halepense and Urochloa panicoides using basal portion of young inflorescence as new and a potential source(s) of explants. Highly embryogenic cultures were initiated on Murashige and Skoog’s (MS) medium supplemented with various concentrations of 2,4-dichlorophenoxyacetic acid (2-4,D). The regenerative cultures were maintained by subculture on MS medium in the dark at 26+2oC. Plants were regenerated via somatic embryogenesis from the competent cell cultures on hormone-free MS basal medium or MS medium with 1mgl-1 kinetin. Somatic seedlings were successfully transplanted to pots and grown to maturity in field. Polyamine levels were analyzed in embryogenic and non-embryogenic cultures. A correlation has been found between the individual polyamine levels and the ratio of putrescine to spermidine in the cultured tissues with plant regeneration ability via somatic embryogenesis

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