Ömer VAROL, Tülin ARSLAN, Köksal KÜÇÜKAKYÜZ, Vatan TAŞKIN, Bekir ÇÖL, Göçmen Belgin TAŞKIN

Esterase polymorphisms in relict endemic Liquidambar orientalis Mill. var. orientalis and L. orientalis Mill. var. integriloba Fiori populations in Turkey

Bu çalışmada, Boreal-Tertiary orijinli relikt endemik L. orientalis populasyonlarında esteraz izoenzim polimorfizmleri incelenmiştir. Türkiye’nin güney ve güney batı bölgelerinden dört populasyon taranmıştır. Bu populasyonlardan ikisi (Fethiye-Günlüklü ve Fethiye-İnlice) L. orientalis Mill.var. orientalis alt türüne aittir. Diğer ikisi ise (Muğla-Köyceğiz ve Antalya-Gebiz) L. orientalis Mill.var. integriloba Fiori alt türüne aittir. Poliakrilamid jelde artan göreceli yürüme değerlerine göre adlandırılan toplam 12 esteraz bandı tespit edilmiştir. Bunların altısı α-esteraz, dördü β-esteraz ve ikisi de α / β-esterazdır. Bu 12 esteraz bandı, dört populasyonda 37 farklı bant deseni oluşturmuştur. Bu 37 farklı bant deseninden sadece dördü bütün populasyonlarda ortaktır. Populasyon içi ve populasyonlar arası çeşitlilik şaşırtıcı derecede yüksek bulunmuştur. Çalışmadan elde edilen sonuçlar, L. orientalis Mill. var. orientalis ve L. orientalis Mill. var. integriloba Fiori alt türlerinin birbirlerinden ayırt edilemediklerini göstermektedir. Esteraz izoenzimlerini biyokimyasal bir belirteç olarak kullanan bu ön çalışma, bu bitki türüne ait literatürde ilk olma özelliği nedeniyle önemlidir.

Türkiye’deki relikt endemik Liquidambar orientalis Mill. var. orientalis ve L. orientalis Mill. var. integriloba Fiori populasyonlarındaki esteraz polimorfizmi

In this study, esterase isozyme polymorphisms in Boreal-Tertiary origin relict endemic L. orientalis populations were investigated. Four populations located in the Southern and the Southwestern regions of Turkey were screened. Two of these populations (Fethiye-Günlüklü and Fethiye-İnlice) belong to subspecies L. orientalis Mill.var. orientalis. The other two (Muğla-Köyceğiz and Antalya-Gebiz) belong to subspecies L. orientalis Mill.var. integriloba Fiori. A total of 12 esterase bands were detected and named according to their increasing relative mobilities on the polyacrylamide gels. Six of them were α-esterases, four of them were β-esterases and the remaining two were α / β-esterases. These 12 esterase bands resulted in 37 different banding patterns in four populations. Only four of these 37 patterns were common among the populations. The levels of variations detected within and among the populations were surprisingly high. According to the results obtained from this study, subspecies L. orientalis Mill. var. orientalis and subspecies L. orientalis Mill. var. integriloba Fiori were not distinguished from each other in any respect. Being the first in the specialized literature of the studied plant species, this preliminary study carried out using esterase isozymes as a biochemical marker is important.

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