Ashwagandha (Withania somnifera) Kök Tozunun Yumurtacı Tavuklarda Performans, Yumurta Kalitesi ve Yumurta Sarısı Lipid Peroksidasyonu Üzerine Etkileri
Bu çalışma, yumurtacı tavuk (Lohmann LSL, 36 haftalık) karma yemlerine farklı düzeylerde (0, 200, 400, 600 mg/kg) ashwagandha (Withania somnifera) kök tozu (ARP) ilavesinin performans, yumurta kalitesi ve yumurta lipid peroksidasyonu üzerine etkilerini belirlemek üzere yürütüldü. Bu amaçla, biri kontrol (ARP-0) ve diğer üçü deneme grubu (ARP-1, ARP-2, ARP-3) olmak üzere toplam 4 grup oluşturuldu. Her grupta 8 tekerrür ve her tekerrürde ise 3’er adet tavuk olacak şekilde toplam 96 adet tavuk 4 katlı kafeslere dağıtıldı. Deneme toplam 2 ay sürdü. Bu süre içerisinde yem ile su ad-libitum olarak verildi ve günlük 16 saat aydınlık 8 saat karanlık fotoperiyot uygulandı. ARP-0 grubu bazal rasyonla, deneme grupları ise bazal yeme sırasıyla ARP’nin 200, 400 ve 600 mg/kg düzeylerinin ilavesi ile hazırlanmış yemlerle beslendi. Yumurta ağırlığı, ak indeksi, Haugh birimi, kabuk ağırlığı, yumurta sarısı L* ile b* ve TBARs (0. gün) değeri hariç diğer parametreler karma yemdeki ARP’den etkilenmedi. En yüksek yumurta ağırlığı (p<0.05) ARP-2; en yüksek ak indeksi (p<0.05), Haugh birimi (p<0.05) ve kabuk ağırlığı (p<0.05) ARP-1 gurubunda oldu. Yumurta sarısı L* (p<0.001) ve b* (p<0.001) değerlerinin en yüksek ARP-1 ve ARP-3 gruplarında olduğu tespit edildi. Karma yemdeki ARP’nin tüm düzeylerinin kontrol grubuna göre yumurta sarısı TBARs (0. gün) değerini azalttığı (p<0.05) tespit edildi. Sonuç olarak, yumurtacı tavuklarda ARP’nin performansı olumsuz olarak etkilemeden yumurtada bazı kalite parametrelerini iyileştirebileceği ve lipid oksidasyonunu geciktirebileceği söylenebilir.
The Effects of Ashwagandha (Withania somnifera) Root Powder on Performance, Egg Quality and Yolk Lipid Oxidation in Laying Hens
This study was conducted to determine the effects of different levels (0, 200, 400, 600 mg/kg) of ashwagandha (Withania somnifera) root powder (ARP) on performance, egg quality and egg lipid peroxidation in laying hens (Lohmann LSL, 36 weeks old) diets. For this purpose, a total of 4 groups were formed, one of which was control (ARP-0) and the other three were experimental groups (ARP-1, ARP-2, ARP-3). A total of 96 hens were distributed to 4-storey cages with 8 replicates in each group and 3 hens in each replicate. The experiment lasted for a total of 2 months. During this period, feed and water were given as ad-libitum and 16 hours of light and 8 hours of dark photoperiod was applied daily. The ARP-0 group was fed with basal diet and the experimental groups were fed with diets prepared by adding 200, 400 and 600 mg/kg levels of ARP to the basal diet, respectively. Except for egg weight, albumen index, Haugh unit, shell weight, yolk L* and b* and TBARs (day 0), other parameters were not affected by ARP in the diet. The highest egg weight (p<0.05) was in the ARP-2 group while the highest albumen index (p<0.05), Haugh unit (p<0.05) and shell weight (p<0.05) were in the ARP-1 group. It was determined that egg yolk L* (p<0.001) and b* (p<0.001) values were highest in ARP-1 and ARP-3 groups. Moreover, it was observed that all levels of ARP in the diet decreased the yolk TBARs (day 0) value compared to the control group (p<0.05). As a result, it can be said that ARP can improve some quality parameters and delay lipid oxidation in laying hens without adversely affecting performance.
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- Akotkar, N.S., Sarag, A. N., Rekhate, D.H. & Dhok, A.P.
(2007). Effect of supplementation of Ashwagandha
(Withania somnifera) on performance of broilers.
Indian Journal of Poultry Science, 42(1), 92-94.
- AOAC. (2000). Official methods of analysis (17th ed.).
Assosication of Official Analytical Chemists,
AOAC International, Maryland, USA.
- Azimi, V., Mirakzahi., M.T. & Saleh, H. (2020).
Hydroalcoholic extract of Withania somnifera leaf
and α-tocopherol acetate in diets containing oxidised
oil: effects on growth performance, immune
response, and oxidative status in broiler chickens,
Italian Journal of Animal Science, 19(1), 917-928.
DOI: 10.1080/1828051X.2020.1808537.
- Bhardwaj, R.K. & Gangwar, S.K. (2011). Effect of dietary
supplementation of Withania somnifera on egg
production and egg quality parameters in Japanese
Quails International Journal of Advanced Biological
Research, 1, 32-34.
- Biswas, P., Sharma, R.K. & Biswas, A. (2012). Effect of
dietary supplementation of Ashwagandha (Withania
somnifera) and ascorbic acid on production
performance of broiler birds. Indian Journal of
Poultry Science, 47(3), 403-405.
- IBM Corp. (2011). IBM SPSS Statistics for Windows,
Version 20.0. IBM Corporation, Armonk, NY.
- Candan, T. & Bağdatlı, A. (2017). Use of natural
antioxidants in poultry meat. Celal Bayar University
Journal of Science, 13(2), 279-291. DOI:
10.18466/cbayarfbe.319752
- Carpenter, K.J. & Clegg, K.M. (1956). The metabolizable
energy of poultry feeding stuffs in relation to their
chemical composition. Journal of the Science of
Food and Agriculture, 7(1), 45-51. DOI:
10.1002/jsfa.2740070109
- Gupta, G.L. & Rana, A.C. (2007). PHCOG MAG.: Plant
review Withania somnifera (Ashwagandha): A
review. Pharmacognosy Reviews, 1(1), 129-136.
- Joshi, S.S., Ingle, P.B., Bhagwat, S.R., Pawar, M.M.,
Prajapati, K.B. & Kulkarni, R.C. (2015). Effect of
dietary addition of ashwagandha (Withania
somnifera) and guduchi (Tinospora cordifolia)
powder on broiler performance. Indian Journal of
Animal Sciences, 85(12), 1358-1361.
- Karasu, K. & Öztürk, E. (2014). Tıbbi ve aromatik bitkilerin
kanatlılarda antioksidan ve antimikrobiyal etkileri.
Türk Tarım ve Doğa Bilimleri Dergisi, 1(Özel Sayı-2), 1766-1772.
- Kılıç, B. & Richards, M.P., (2003). Lipid oxidation in
poultry doner kebap: prooxidative and antioxidative
factors. Journal of Food Science, 68(2), 686-689.
DOI: 10.1111/j.1365-2621.2003.tb05732.x
- Kumar, S., Berwal, R.S. & Sihag, S. (2020). Effect of
dietary supplementation of ashwagandha root
powder on production performance, egg quality and
blood biochemical constituents of laying hens.
Indian Journal of Animal Nutrition, 37(4), 352-357.
DOI: 10.5958/2231-6744.2020.00057.2
- Kumar, S. (2021). Effect of dietary supplementation of
Ashwagandha (Withania somnifera) root powder on
external egg parameters and body weight changes in
laying hens. The Pharma Innovation Journal, 10(9),
475-478.
- Kushwaha, S., Betsy, A. & Chawla, P. (2012). Effect of
Ashwagandha (Withania somnifera) root powder
supplementation in treatment of hypertension.
Studies on Ethno-Medicine, 6(2), 111-115. DOI:
10.1080/09735070.2012.11886427
- Manuelian, C.L., Pitino, R., Simoni, M., Mavrommatis, A.,
De Marchi, M., Righi, F. & Tsiplakou, E. (2021).
Plant feed additives as natural alternatives to the use
of synthetic antioxidant vitamins on livestock
mammals’ performances, health, and oxidative
status: A Review of the Literature in the Last 20
Years. Antioxidants, 10(9), 1461. DOI:
10.3390/antiox10091461
- Mishra, L.C., Singh, B.B. & Dagenais, S. (2000). Scientific
basis for the therapeutic use of Withania somnifera (ashwagandha): a review. Alternative Medicine
Review, 5(4), 334-346.
- NRC. (1994). Nutrient requirements of poultry. 9th rev. Ed.,
National Academy Press. Washington, D.C.
- Özgan, A. (2008). Fonksiyonel Yumurta Eldesinde Üzüm
Çekirdeği Yağının Kullanım Olanakları. Yüksek
Lisans Tezi, Çukurova Üniversitesi Fen Bilimleri
Enstitüsü. Adana, Türkiye, 69s.
- Peşman, E., Yıldız, H.E., Boran Torun, S. & Dönmez
Çavdar, A. (2022). Atık gazete kağıdı liflerinin
biyokompozit üretiminde kullanılabilirliği. Artvin
Çoruh Üniversitesi, Orman Fakültesi Dergisi, 23(1),
45-50. DOI: 10.17474/artvinofd.1027482
- Righi, F., Pitino, R., Manuelian, C.L., Simoni, M.,
Quarantelli, A., De Marchi, M. & Tsiplakou, E.
(2021). Plant feed additives as natural alternatives to
the use of synthetic antioxidant vitamins on poultry
performances, health, and oxidative status: A review
of the literature in the last 20 years. Antioxidants,
10(5), 659. DOI: 10.3390/antiox10050659
- Ryu, K.N., No, H.K. & Prinyawiwatkul, W. (2011). Internal
quality and shelf life of eggs coated with oils from
different sources. Journal of Food Science, 76(5),
325-329. DOI: 10.1111/j.1750-3841.2011.02177.x
- Sandeep, K., Berwal, R.S. & Ravi, K. (2020). Effect of
dietary supplementation of Ashwagandha root
powder on production performance of laying hens.
Haryana Veterinarian, 59(2), 201-205.
- Sarıca, M. & Erensayın, C., (2009). Tavukçuluk ürünleri:
tavukçuluk bilimi, yetiştirme, besleme ve
hastalıklar. Ed: Türkoğlu, M. ve Sarıca M., 89-139.
Bey Ofset Matbaacılık, Ankara.
- Vasanthakumar, P., Pangayarselvi, B., Sasikumar, P.,
Chandrasekaran, D., Doraisamy, K.A. &
Purushothaman, M.R. (2015). Performance of
broilers fed ashwagandha (Withania somnifera)
incorporated diets during summer season for
alleviating heat stress. Indian Journal of Animal
Research, 2646, 1-3. DOI: 10.5958/0976-
0555.2015.00082.5
- Yalçın, S., Özsoy, B., Erol, H. & Yalçın, S. (2008). Yeast
culture supplementation to laying hen diets
containing soybean meal or sunflower seed meal its
effect on performance, egg quality traits, and blood
chemistry. Journal of Applied Poultry Research,
17(2), 229-236. DOI: 10.3382/Japr.2007-00064
- Yalçın, S., Erol, H., Özsoy, B., Onbaşılar, İ., Yalçın S. &
Üner, A. (2010). Effects of glycerol on performance,
egg traits, some blood parametersa and antibody
production to SRBC of laying hens. Livestock
Science, 129, 129-134. DOI:
10.1016/j.livsci.2010.01.014.