Saeed NAZIFI, Khalil BADIEI, Mehrdad POURJAFAR, Aliasghar CHALMEH

Anti - inflammatory effects of insulin and dexamethasone on Experimentally Escherichia coli serotype O55:B5 induced endotoxemia in Iranian fat - tailed sheep

Bu deneyde yağlı kuyruklu İran koyunlarında Escherichia coli serotip O55:B5’e ait endotoksinlerin intravenöz infüzyonunu takiben insulin ve deksametazonun endotoksemi tedavisindeki etkileri karşılaştırıldı. Yirmi adet klinik olarak sağlıklı bir yaşındaki yağlı kuyruklu İran koyunların tesadüfi seçimiyle Kontrol, Dexa, Insln 1,5 ve Insln 3’ten oluşan dört (n=5) adet deney grubu oluşturuldu. Escherichia coli serotip O55:B5’in lipopolisakkaridi 20 μg/kg dozda intravenöz olarak uygulandı. Tüm deney gruplarına lipopolisakkarit enjeksiyonu sonrası 120 dakika süreyle intravenöz sıvı tedavisi uygulanmıştır. Lipopolisakkarid enjeksiyonundan 180 dakika sonra, deksametazon (Dexa grubuna 1 mg / kg) ve insülin (Insln 1,5 ve 3 gruplarına sırasıyla, 1,5 ve 3 IU / kg) 60 dakika süreyle damar içi sıvı ile birlikte enjekte edildi. Kontrol grubuna sadece lipopolisakkaritten oluşan ve herhangi bir ilacın kullanılmadığı intravenöz sıvı tedavi uygulandı.Tüm koyunlardan kan örnekleri toplanarak elde edilen serumlardan akut faz proteinleri (serum amiloid A ve haptoglobin), inflamatuar sitokinler (tümör nekroz faktörü-alfa ve interferon-gamma) ve oksidatif stres belirteçleri (süper oksit dismutaz ve glutatyon peroksidaz) değerleri ölçülmüştür. Bütün deney grupları içinde endotoksemi indüksiyondan sonra (P

Yağlı kuyruklu İran koyunların da deneysel Escherichia coli serotip O55:B5'e bağlı endotoksemide insülin ve deksametazonun anti inflamatuar etkileri

The present experiment was performed in order to compare the effect of insulin and dexamethasone on treating endotoxemia following the intravenous infusion of endotoxin of Escherichia coli serotype O55:B5 in the Iranian fattailed sheep. Twenty clinically healthy one-year old Iranian fat-tailed ewes were randomly assigned into four equal (n=5) experimental groups, comprising of Control, Dexa, Insln 1.5 and Insln 3 groups. Lipopolysaccharide from Escherichia coli serotype O55:B5 was administered intravenously at 20 μg/kg. All experimental groups undergone intravenous fluid therapy for 120 minutes after lipopolysaccharide injection. 180 minutes after lipopolysaccharide injection, dexamethasone (in Dexa group at 1 mg/kg) and insulin regular (in Insln 1.5 and 3 at 1.5 and 3 IU/kg, respectively) were injected along with the intravenous fluid for 60 minutes. The Control group just received lipopolysaccharide and was treated only with intravenous fluid without using any drug. The blood samples were collected from all ewes and assayed separated sera for serum acute phase proteins (serum amyloid A and haptoglobin), inflammatory cytokines (tumor necrosis factor-alpha and interferon-gamma) and oxidative stress biomarkers (super oxide dismutase and glutathione peroxidase). In all experimental groups a rapid increase was seen in the amount of acute phase proteins and inflammatory cytokines and a decrease was seen in oxidative stress biomarkers after endotoxemia induction (P

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