Saeed NAZIFI, Ameneh KHOSHVAGHTI, Mohammad Rafie VALIZADEH, Mohsen VASEI, Bijan AKBARPOUR

The effects of Dorema aucheri hydroalcoholic extract on blood levels of antioxidant enzymes (SOD and GPX) and vitamins (E and C) in vivo

Dorema aucheri, Apiaceae familyasından flavonoid ve kumarinler gibi çeşitli komponentleri içeren bir bitkidir. Flavonoidler antitümöral, antikanserojenik ve östrojenik özelliklerine sahiptir. Kumarinlerin antiandrojenik özellikleri de bilinmektedir. Bu araştırmanın amacı, Dorema aucheri hidroalkolik ekstraktlarının SOD ve GPX aktiviteleri ile E ve C vitaminlerinin serum konsantrasyonları üzerine etkilerini araştırmaktır. 60 adet, 220-220 g ağırlığında ergin Wistar sıçanı 15 adetlik 4 gruba ayrılmıştır. Kontrol grubuna herhangi bir ekstrakt verilmemiştir. Diğer üç deneme grubuna 28 gün boyunca ağız yoluyla, düşük (100 mg/kg CA), orta (200 mg/kg CA) ve yüksek (400 mg/kg CA) düzeyde Dorema aucheri hidroalkolik ekstraktı verilmiştir. Tüm gruplarda bulunan hayvanlar 14 ve 28 gün sonra tartılmış ve alınan kanlarından SOD ve GPX aktiviteleri ile vitamin E ve C konsantrasyonları valide edilmiş standart metodlar ile ölçülmüştür. Elde edilen sonuçlar, kısa vadede SOD ve GPX aktivitelerinin yanısıra vitamin C konsantrasyonunun doza bağlı olarak arttığını, ancak uzun vadede bu parametrelerin doz artışı ile orantılı olarak azaldığını göstermiştir. Çalışma esnasında E vitamini konsantrasyonu düzeylerinde anlamlı değişiklik gözlenmemiştir.

Dorema aucheri hidroalkol ekstraktının in vivo kan antioksidan enzimleri (SOD ve GPX) ve vitamin (E ve C) düzeyleri üzerine etkisi

Dorema aucheri is a plant from the Apiaceae family, which has several compounds such as flavonoids and kumarines. Flavonoids have antitumor, anticancer and estrogenic properties. Antiandrogenic properties of kumarines are also known. The aim of this research is to examine the Dorema aucheri hydroalcoholic extract effects on SOD and GPX activities and serum concentration of vitamins E and C. Sixty adult male Wistar rats weighing about 200 to 220 g were divided into four groups of fifteen. The control group did not receive any drug. The other three experimental groups including low (100 mg/kg BW), medium (200 mg/kg BW) and maximum (400 mg/kg BW) received Dorema aucheri hydroalcoholic extract daily for 28 days orally. After 14 and 28 days all animals in the different groups were weighed and their blood collected, and SOD and GPX activities, and vitamin E and C concentrations were measured by validated standard methods. The results showed that in the short term, SOD and GPX activities, as well as vitamin C concentration increase depending on the dose, but in the long term these parameters decrease proportional to the increase of the dosage. Vitamin E concentration had no significant changes during our study.

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