Hücre Zarı Sinyalinin NMR Spektroskopisi ile Belirlenmesi

Hücre zarı, hücrenin sınırlarını oluşturur ve hücre içi ile dışı arasındaki madde geçişini düzenler. Hücrenin kendisi için gerekli besin maddelerini dış ortamdan alıp metabolizma sonucu oluşan zararlı metabolitleri dış ortama verme olayında hücre zarlarının büyük bir rolü bulunmaktadır. Bu çalışmada sulu çözeltideki bir hücre zarı örneğinin 400 MHz 1H-NMR spektrumu, tek puls ve presaturasyon metodu kullanılarak alınmış ve bu iki spektrumun karşılaştırmasıyla hücre zarı sinyalinin yeri saptanmaya çalışılmıştır.

Anahtar Kelimeler:

Hücre zarı, nükleer manyetik rezonans, presaturasyon metodu

Determination of Cell Membrane Signal by using NMR Spectroscopy

The cell membrane forms the boundaries of the cell and regulates the passage of matter between the inside and the outside of the cell. Cell membranes play a major role in the process of taking the necessary nutrients from the outside and exerting the harmful metabolites resulting from the metabolism. In this study, 400 MHz 1H-NMR spectrum of a membrane sample in an aqueous solution was taken using single pulse and presaturation method and it has been tried to determine the location of the membrane signal by comparing these two spectrums.

Keywords:

Cell membrane, presaturation method, nuclear magnetic resonance,

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