Effects of oral and topical Momordica charantia-Propolis premix on wound healing

Momordica charantia ve Propolis'in (MCP) antibakteriyel, antifungal, antioksidan ve antienflamatuar özelliklere sahip olduğu bildirilmektedir. Bu çalışmada, 60 yetişkin erkek sıçanda MCP'nin oral ve topikal uygulanmasının yara iyileşmesi üzerindeki etkinliğinin belirlenmesi amaçlanmıştır. Çalışmada kontrol sıçanlarındaki yaralar tedavi edilmeden bırakıldı, MCP-oral gruptaki sıçanlar oral MCP ile tedavi edildi ve MCP topikal grubundaki hayvanlar, yara üzerine MCP tozu dökülerek tedavi edildi. Yara boyutu 3., 7., 14. ve 21. günlerde ölçüldü ve her iki gruptaki beş hayvandan doku örnekleri alındı. Doku örnekleri histopatolojik olarak değerlendirildi. Takip süresi boyunca tedaviyi takiben remisyon, her sıçan için orijinal yara boyutunun yüzdesi olarak tanımlandı. Verilerin analizi için iki yönlü bir ANOVA kullanıldı. Ortalama yara yüzey alanı 1.69 cm2 olarak ölçüldü. Deneyin sonunda ortalama azalma MCP-topikal grubunda %81.40 ve MCP-oral grubundaki yaralarda%87.85 idi. Yara boyutundaki en düşük azalma kontrol grubunda (%45.55) ölçüldü. Yara boyutundaki küçülme, deney sonunda MCP grupları arasında önemli ölçüde farklı değildi. 21. güne kadar hem MCP-oral hem de MCP-topikal gruplarda yeniden epitelizasyon ve neovaskülarizasyon tamamlandı. Genel olarak, oral ve topikal MCP ile yapılan tedaviler yara iyileşmesinde eşit derecede etkilidir. Momordica charantia ve Propolis'in hem oral hem de topikal uygulamaları yara iyileşmesinin hızlandırılması için düşünülebilir.

___

  • Abramov Y, Golden B, Sullivan M, Botros SM, Miller JJR et al, 2007: Histologic characterization of vaginal vs. abdominal surgical wound healing in a rabbit model. Wound Repair and Regeneration, 15 (1), 80-86.
  • Agren MS, Mertz PM, Franzen, L, 1997: Acomparative study of three occtusive dressing in the treatment of full thickness wounds in pigs. J Am Acad Dermatol, 36, 53-58.
  • Ahmad Z, Zamhuri KF, Yaacob A, Siong CH, Selvarajah M et al, 2012: In vitro anti-diabetic activities and chemical analysis of polypeptide-k and oil isolated from seeds of Momordica charantia (bitter gourd). Molecules, 17 (8), 9631-9640.
  • Anderson DM, White RAS, 2000: Ischemic bandage injuries: A case series and review of the literature. Vet Surg, 29 (6), 488-498.
  • Atayoglu AT, Sılıcı S, 2016: Preliminary Study on Wound Healing Activity of Propolis in Albino Rats. International Journal of Innovative Research in Medical Sciences (IJIRMS), 1 (7), 2455-8737.
  • Basim E, Basim H, Özcan M, 2006: Antibacterial activities of Turkish pollen and propolis extracts against plant bacterial pathogens. Journal of Food Engineering, 77 (4), 992-996.
  • Baum CL, Arpey CJ, 2005: Normal cutaneous wound healing: clinical correlation with cellular and molecular events. Dermatol Surg, 31, 674-686.
  • Das DR, Sachan AK, Imtiyaz M, Shuaib M, 2015: Momordica charantia as a potential medicinal herb: an overview. J. Med. Plants Stud, 23 (25), 23-26.
  • Diegelmann RF, Evans MC, 2004: Wound healing: an overview of acute, fibrotic and delayed healing. Front in Biosci, 9(1), 283-289.
  • Grover JK, Yadav SP, 2004: Pharmacological actions and potential uses of Momordica charantia: a review. J Ethnopharmacol, 93, 123-132.
  • Gu Y, Kaida T, Kaida K, 2005: Immunostimulating and Antitumor Effects by Inonotus obliquus (Ach.:Pers.) Pilat. Int J Med Mushrooms, 7 (3), 406.
  • Hussan F, Teoh SL, Muhamad N, Mazlan M, Latiff AA, 2014: Momordica charantia ointment accelerates diabetic wound healing and enhances transforming growth factor-β expression. J wound care, 23 (8), 400-407.
  • Keast D. Ve Orsted H, 2000: The basic principles of wound healing. Ostomy Wound Management, 46 (11), 16.
  • Keskin N, Hazır S, Baser KHC, Kurkcuoglu M, 2001: Antibacterial activity and chemical composition of Turkish propolis. Z Naturforsch, 56 (11-12), 1112-1115.
  • Kim J, Lee CM, 2017: Wound healing potential of a polyvinyl alcohol-blended pectin hydrogel containing Hippophae rahmnoides L. extract in a rat model. Int J Biol Macromol, 99, 586-593.
  • Kim YS, Cho IH, Jeong MJ, Jeong SJ, Nah SY et al, 2011: Therapeutic effect of total ginseng saponin on skin wound healing. J Ginseng Res, 35 (3), 360-367.
  • Koc AN, Silici S, Ayangil D, Ferahbaş A, Çankaya S, 2005: Comparison of in vitro activities of antifungal drugs and ethanolic extract of propolis against Trichophyton rubrum and T.mentagrophytes by using a microdilution assay. Mycoses, 48 (3), 205-210.
  • Lemo N, Marignac G, Reyes-Gomez E, Lilin T, Crosaz O et al, 2010: Cutaneous reepithelialization and wound contraction after utaneous reepithelialization and wound contraction after skin biopsies in rabbits: a mathematical model for healing and kin biopsies in rabbits: a mathematical model for healing and remodelling index. Vet Arh, 80 (5), 637-652.
  • Mahmood AA, Mariod AA, Abdelwahab SI, Ismail S, Al-Bayaty F, 2010: Potential activity of ethanolic extract of Boesenbergia rotunda (L.) rhizomes extract in accelerating wound healing in rats. J Med Plant Res, 4 (15), 1570-1576.
  • Miyataka H, Nishiki M, Matsumoto H, Fujimoto T, Matsuka M et al, 1997: Evaluation of propolis. Part I: evaluation of Brazilian and Chinese propolis by enzymatic and phsico- chemical methods. Biol Pharm Bull, 20 (5), 496-501.
  • Ono T, Tsuji T, Sakai M, Yukizaki C, Ino H et al, 2009: Induction of hepatocyte growth factor production in human dermal fibroblasts and their proliferation by the extract of bitter melon pulp. Cytokine, 46 (1), 119-126.
  • Oryan A, Alemzadeh E, Moshiri A, 2018: Potential role of propolis in wound healing: Biological properties and therapeutic activities. Biomed Pharmacother, 98, 469-483.
  • Otranto M, Do Nascimento AP, Monte-Alto-Costa A, 2010: Effects of supplementation with different edible oils on cutaneous wound healing. Wound Repair Regen, 18(6), 629-636.
  • Pillai SI, Palsamy P, Subramanian S, Kandaswamy M, 2010: Wound healing properties of Indian propolis studied on excision wound-induced rats. Pharmaceutical Biology, 48 (11), 1198-1206.
  • Ragab AI, Mekkawy MM, El-Gamil A, Mohanny KM, 2015: Effect of Propolis Dressing Technique on the Healing of Septic Diabetic Foot Ulcers. IOSR Journal of Nursing and Health Science (IOSR-JNHS4), 4, 33-52.
  • Ramos AFN, Miranda JL. 2007: Propolis: a review of its anti-inflammatory and healing actions. J Venom Anim Toxins Incl Trop Dis,13(4), 697-710.
  • Sahu RK, Jain A, Nayak S. 2011: Momordica charantia Linn.: a mini review. Int J Biomed Res, 11 (2), 579-587.
  • Sankaranarayanan J, Jolly CI, 1993: Phytochemical, antibacterial and pharmacological investigations on Momordica charantia Linn. Emblica officinalis gaertn. and Curcuma longa Linn. Indian Journal of Pharmaceutical Sciences, 55 (1), 6-13.
  • Sıngla V, Saını A, Joshı B, Rana AC, 2012: Emulgel: A new platform for topical drug delivery. International Journal of Pharma and Bio Sciences, 3 (1), 485-498.
  • Simões LMC, Gregório LE, Da Silva Filho AA, de Souza ML, Azzolini AECS et al, 2004: Effect of Brazilian green propolis on the production of reactive oxygen species by stimulated neutrophils. J Ethnopharmacol, 94 (1), 59-65.
  • Singh R, Garcia-Gomez I, Gudehithlu KP, Singh AK, 2017: Bitter Melon Extract Promotes Granulation Tissue Growth and Angiogenesis in the Diabetic Wound. Adv Skin Wound Care, 30 (1), 16-26.
  • Velnar T, Baıley T, Smrkolj V, 2009: The wound healing process: an overwiev of the cellular and molecular mechanisms. The Journal of International medical research, 37: 1528-1542.
  • Wang, L. Chen, W. Wu, Y. Long, R. Wang, 2008: Potential cytoprotection: antioxidant defence by caffeic acid phenethyl ester against free radical-induced damage of lipids, DNA, and proteins. Canadian Journal of Physiology and Pharmacology, 86 (5), 279-287.
  • Zhang QC, 1992: Preliminary report on the use of Momordica charantia extract by HIV patients. Journal of Naturopathy Medicine, 3, 65-69.