Deneysel Diyabetik Nefropatide Patomorfoloji ve Apoptozis

Bu çalışmada diyabetik nefropatiye bağlı oluşan böbrek hasarında histopatolojik ve apoptotik bulguların değerlendirilmesi amaçlandı. Çalışmada 16 rat rastgele olarak kontrol (n=8) ve üç gün arayla iki kez streptozisinle (60 mg/kg, i.p) uyarılmış diyabet (n=8) olmak üzere iki gruba ayrıldılar. Son uygulamayı takip eden 90. günde ratlar uyutularak böbrekleri patolojik incelemeler amacıyla alındı. Dokulara histopatolojik olarak hematoksilen-eozin ve immunohistokimyasal olarak kaspas-3, kaspas-8, kaspas-9 ve TUNEL işaretlemeleri yapıldı. Histopatolojik olarak, tubullerde dilatasyon, atrofi, dejenerasyon, nekroz, glomeruluslarda ise hipertrofi ve vasküler değişikler görüldü. İntersitisyel alanlarda ise yer yer peritubuler, perivasküler ve periglomeruler fibrozis ve inflamasyonlar gözlendi. İmmunohistokimyasal olarak, kaspas-3’ün birçok tubulde pozitif olduğu ayrıca glomerullerde ve intersitisyumda tek tük pozitif hücre ile karşılaşıldı. Kaspas-8 ve kaspas-9 antikorlarının ise birçok tubulde reaktif oldukları belirlendi. TUNEL boyamalarında ise yaygın pozitiflik tubulerde, yer yer intersitisyumda ve glomeruluslarda belirlenmiştir. Sonuç olarak, diyabete bağlı oluşan nefropatide bütün böbrek dokusunda hasarın şekillendiği belirlenmiştir.

Anahtar Kelimeler:

Diyabetik nefropati, patomorfoloji, apoptozis

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