Pendimethalin stresine maruz kalan aspir (Carthamus tinctorius L.)’de oluşan bazı biyokimyasal değişiklikler

Pendimethalin herbisiti tarımsal uygulamalarda yabancı otların kontrolü için yaygın olarak kullanılmaktadır. Bununla birlikte, pendimethalinin kültür bitkileri üzerindeki yan etkisi tam olarak bilinmemektedir. Bu çalışmada, aspir (Carthamus tinctorius L.) bitkisine ait Dinçer ve Remzibey çeşitlerinde pendimethalin kaynaklı oksidatif stres araştırıldı. Herbisit uygun büyüklüğe (21 günlük) gelen bitkilerin yapraklarına toksisite denemeleri sonrasında belirlenen dozlarda (0.004-0.01 M) çimlenme sonrası olarak uygulandı. Uygulama gruplarında karotenoid, toplam klorofil, toplam karbohidrat, toplam fenolik ve lipid peroksidasyonunu gösteren malondialdehid (MDA) içeriğindeki değişimler saptandı. Dinçer (% 42.7) ve Remzibey (% 59) çeşitlerinde karotenoid içeriği kontrole kıyasla azalış gösterdi. Toplam klorofil içeriği Remzibey çeşidinde (% 56.8) Dinçer’e (% 41.4) göre daha yüksek bulundu. Toplam karbohidrat içeriği Dinçer çeşidinde (% 50) Remzibey çeşidine (% 46.1) kıyasla daha yüksek saptandı. Toplam fenolik içeriği Remzibey (% 48.3) ve Dinçer (% 46.7) çeşitlerinde artan saatlere bağlı olarak azalış gösterdi. MDA içeriği Remzibey çeşidinde (% 72) Dinçer çeşidine (% 70) göre daha yüksek saptandı. Bu çalışma, pendimethalinin kültür bitkisi olan aspire toksik etki oluşturduğunu göstermiştir.

Anahtar Kelimeler:

Aspir, Carthamus tinctorius, Pendimethalin, Malondialdehid, Karotenoid

Some biochemical changes in safflower (Carthamus tinctorius L.) plant exposed to pendimethalin stress

Pendimethalin herbicide is widely used for controlling weeds in agricultural applications. However, the side effect of pendimethalin on cultivated plants is unknown. In this study, oxidative stress induced by pendimethalin in safflower plant (Carthamus tinctorius L.) was investigated. In this study, oxidative stress induced by pendimethalin in Dinçer and Remzibey cultivars of safflower was investigated. The herbicide was applied after germination at the doses determined (0.004-0.01 M) following toxicity tests to the leaves of plants of suitable size (21 days). In application groups, carotenoids, total chlorophyll, total carbohydrate, total phenolic and malondialdehyde (MDA) content indicating lipid peroxidation were investigated. The content of carotenoid in Dinçer (42.7 %) and Remzibey (59 %) cultivars decreased compared to control. Total chlorophyll content was significantly higher in Remzibey cultivar (56.8 %) than Dinçer cultivar (41.4 %). In Dinçer cultivar (50 %), carbohydrate content was found to be higher than Remzibey cultivar (46.1 %). Total phenolic content decreased with increasing hours in Remzibey (48.3 %) and Dinçer (46.7 %) cultivars. MDA content was higher in Remzibey cultivar (72.4 %) than Dinçer cultivar (70.1 %). This study has shown that pendimethalin herbicide has a toxic effect on safflower, which is a cultural plant.

Keywords:

Safflower, Carthamus tinctorius, Pendimethalin, Malondialdehyde, Carotenoid,

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Boukehili, K., Khaldi, F., Kharrachi, M., 2015. Impact of two herbicides (Zoom and Sencorate) on physiological parameters (chlorophyll, proline and total protein), enzymatic (CAT and APX) and non- enzymatic biomarkers (MDA and GSH) of the species Orthotrichum affine International Journal of Plant & Soil Science 4 (4) : 352–365.
Breusegem, F.V., Dat, J.F., 2006. Reactive oxygen species in plant cell death. Plant Physiol. Jun. 141 (2): 384–390.
Büyük, İ., Soydam-Aydın, S., Aras, S., 2012. Bitkilerin stres koşullarına verdiği moleküler cevaplar. Türk Hij Den Biyol Derg. 69 (2): 97–110.
Chandler, S.F., Dodds, J.H., 1983. The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasidine in callus cultures of Solanum lacinitum, Plant Cell Reports. 2: 105–108.
Dajue, L., Mundel, H.H., 1996. International Plant Genetic Resources Institute.
De-Kok, L., Graham, M., 1980. Levels of pigments, soluble proteins, amino acids and sulfhydryl compounds in foliar tissue of Arabidopsis thaliana during dark induced and natural senescence. Plant Physiology and Biochemistry. 27: 133–142.
Delen, N., Durmuşoğlu, E., Güncan, A., Turgut, C., Güngör, N., Burçak, A., 2005. Türkiye’de pestisit kullanımı ve organizmalarda duyarlılık azalışı sorunları, Türkiye Ziraat Mühendisliği 6. Teknik Kongre, 1–21.
Dragičević V., Simić M., Stefanović L., Sredojević S., 2010. Possible toxicity and tolerance patterns towards post-emergence herbicides in maize inbred lines. Fresenius Environmental Bulletin. 19: 1499–1504.
Duncan, D.B., 1955. Multiple range and multiple F tests biometrics. Int. Biom. Soc. 11(1): 1– 42.
Erdoğan, B.Y., 2010. Samsun’da Yaygın Olarak Kullanılan Pestisitlerin Sağlığa ve Çevreye Etkileri Alınteri 19 (B) –28-35 ISSN:1307-3311.
Ghanizadeh, H., Harrington, K.C., James, T.K., Woolley, D.J., Ellison, N.W., 2015. Mechanisms of glyphosate resistance in two perennial ryegrass (Lolium perenne) populations. Pest Manag Sci. 71(12): 1617-22.
Gilbert, J., 2008. International safflower production – An Overview. 7. International Safflower Conference. Australian Oilseeds Federation. Wagga Wagga, Australia.
Heath, R.L., Packer, L., 1968. Photoperoxidation in isolated chloroplast, I. kinetics stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125: 180.
Hoagland, D.R., Arnon, D.I., 1938. The water culture method for growing plants without soil, Circ. Calif. Agr. Exp. Sta., 347: 461.
Jha, P., Kumar, V., Lim, A. Charlemagne, Yadav R., 2017. Evaluation of preemergence herbicides for crop safety and weed control in Safflower. American Journal of Plant Sciences, 2017, 8, 2358-2366
Kıslalıoğlu, M., Berkes, F., 1985. Ekoloji ve Çevre Bilimleri: Türkiye Çevre Sorunları Vakfı Yayını, Ankara, 361.
Langaro, A.C., Agostinetto, D., Ruchel Q., Garcia, J. R., Perboni L.T., 2017. Oxidative stress caused by the use of preemergent herbicides in rice crops. Revista Ciência Agronômica. 48 (2) 358–364.
Levitt, J., 1972. Responses of plants to environmental Stresses. New York, London: Academic Press, 697.
Li, G., Wan, S., Zhou, J., Yang, Z., Qin, P., 2010. Leaf chlorophyll fluorescence, hyperspectral reflectance, pigments content, malondialdehyde and proline accumulation responses of castor bean (Ricinus communis L.) seedlings to salt stress levels. Industrial Crops and Products. 31: 13–19.
Lichtenthaler, K., Welburn, A.R., 1983. Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Botanisches Institutder Univeristat, Kaiserstran βe 12, Postfach. 591–592.
Macedo, R.S., Lombardi A.T., Omachi, C.Y., Rorig, L.R., 2008. Effects of the herbicide bentazon on growth and photosystem II maximum quantum yield of the marine diatom Skeletonema costatum, Toxicology in Vitro. 22: 716–722.
Malencic, D.R., Miladinović, J.A., Popović, M.T., 2008. Effects of linuron and dimethenamid on antioxidant systems in weeds associated with soybean. Central European Journal of Biology, June. Volume 3, Issue 2, 155–160.
Marchezan, M.G., Avila, L.A., Agostinetto, D., Schaedler, C.E., Langaro A.C., Oliveira C., Zimmer, M., Schreiber, F., 2017. Morphological and biochemical alterations of paddy rice in response to stress caused by herbicides and total plant submersion. Planta Daninha. 1-13.
Mengistu, L.W., Mueller-Warrant, G.W., Liston, A., Barker, R.E., 2000. psb mutation (valine219 to isoleucine) in Poa annua resistant to metribuzin and diuron, Pest Manag. Sci. 56: 209–217.
Nohatto, M.A., Dirceu, A., Langaro, A. C., Oliveira, Claudia de, Ruchel, Q., 2016. Antioxidant activity of rice plants sprayed with herbicides. e-ISSN 1983-4063 - www.agro.ufg.br/pat - Pesq. Agropec. Trop., Goiânia, 46: 1, 28–34.
Romani, A., Vignolini, P., Galardi, C., Aroldi, C., Vazzana, C., Heimler D., 2003. Polyphenolic content in different plant parts of soy cultivars grown under natural conditions. J. Agric. Food Chem. 51: 5301–5306.
Rosenberg, S., 1980. Physiological studies of lignocellulose degratation by thermotolerant mold Chrysosporium prunosum, Symposium on the biological transformation of lignocellulose, 12: 133–142.
Saladin, G, Magnea, C., Cleament, C., 2003. Effects of flumioxazin herbicide on carbon nutrition of Vitis vinifera L., J. Agric. Food Chem., 51: 4017–4022. Slinkard, K., Singleton V.L., 1977. Total phenol analyses: automation and comparison with manual methods, American Journal of Enology and Viticulture. 28: 49–55.
Smirnoff, N., 1993. The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol. 125: 27–58. Squibb, K., 2002. Pesticides, Program in Toxicology NURS 678–Applied Toxicology. 1–48.
Türkan, İ., Bor, M., Özdemir, F., Koca, H., 2005. Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress Plant Science 168, 223–231.
Van Breusegem, F., Dat, J.F. 2006. Reactive oxygen species in plant cell death. Plant Physiol, 141: 384–90.
Van Camp, W., Van Montagu, M., Inze, D., 1998. H2O2 and NO: Redox signals in disease resistance. Trends Plant Sci, 3: 330–4.
Zhou, Q., Liu W., Zhang Y., Liu K.K., 2007. Action mechanisms of acetolactate synthase-inhibiting herbicides, Pesticide Biochemistry and Physiology, 89: 89–96.