The effects of time, temperature, solvent: solid ratio and solvent composition on extraction of total phenolic compound

Kurutulmuş zeytin yaprağından toplam fenolik maddelerin ekstraksiyonu için optimum ekstraksiyonkoşulları cevap yüzey yöntemi kullanılarak belirlenmiştir. Çözücü kompozisyonu (etanol-su, %20 - %100),ekstraksiyon sıcaklığı (20 - 60 °C), ekstraksiyon süresi (4 - 48 sa) ve çözücü katı oranı (4 - 8) gibi bağımsızdört farklı değişkenin etkisinin belirlenmesinde merkezi teşekküllü dizayn kullanılmıştır. Cevap olarakekstraktaki toplam fenolik madde içeriği seçilmiştir. Sonuçlar ikinci dereceden denklem ile açıklanmış veR20.8539 olarak bulunmuştur. Ekstraçekleştirilmesi için parametrelerin minimize maksimize edilmesiveya aralığının seçilebilmesi nedeniyle çeşitli çözümler yapılabilmektedir. Bu çalışmada zeytin yaprağındantoplam fenolik madde ekstraksiyonu için önerilen seçilmiş koşullar %43 etanol içeren su (h/h), 50 ºC, 15sa ve 7 kat çözgen/katı oranı olarak belirlenmiştir. Optimum koşullar uygulanarak belirlenen kurutulmuşzeytin yaprağının toplam fenolik madde içeriği ise 4586.3 mg GAE/100 g kuru yaprak olarak bulgulanmıştır.

Çözücü Kompozisyonunun Etkisi (İngilizce) Kurutulmuş zeytin yaprağından (olea europaea L.) toplam fenoloik madde ekstraksiyonu üzerine süre, sıcaklık, çözücü-katı oranı ve çözücü kompozisyonunun etkisi

The optimum extraction conditions for total phenolic compounds from dried olive leaves were determinedby using response surface methodology. Central composite design was used to investigate the effects of fourindependent variables such as solvent composition (ethanol in water, 20 to 100%), extraction temperature(20 to 60 °C), extraction time (4-48 hours) and solvent/solid ratio (4 to 8). As a response total phenoliccompound content of the extracts were chosen. The quadratic model is used for predicting the results andR2was found 0.8539. The olive leaves with the initial 43.94±0.25% moisture content were dried in vacuumoven at 60 ºC to 2±0.07% moisture content before extraction. There are several solutions by choosing thetarget, in range, maximize or minimize the effective parameters in response surface methodology. In thisstudy, recommended optimal conditions for the total phenolic compounds from olive leaves were found43% ethanol in water (v/v), 50 ºC, 15 hours and 7 times solvent/solid ratio. Under the chosen optimumconditions the corresponding predicted response value for total phenolic compounds was 4586.3 mgGAE/100 g dried leaves.

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