Proteins in honey

Güneydoğu Anadolu bölgesinde toplanılan bal örneklerindeki proteinler sodyum dodesil sülfat poliakrilamid jel elektroforez (SDS-PAGE) metodu ile belirlendi. Bal örneklerinde molekül ağırlığı yaklaşık 22.000 - 75.000 arasında olan 12 farklı protein bandı görüldü. Ayrıca bu bal örneklerin hidrokslmetilfurfural, diastaz sayısı, invert şeker, sakaroz, prolin, nem, kül, serbest asit, pH ve lakton değerleri de tayin edildi.

Bal proteinleri

The proteins of honey from South-Eastern Anatolia were identified by sodium dodecyl sulfate-polyacrylamld gel electropheresis (SDS-PAGE). It was found 12 protein bands in various honey samples. The molecular weights of these proteins were determined approximately between 22,000 and 75,000. Also, determined in the honey samples were hydroxymethylfurfural, diastase number, invert sugar, sucrose, proline, moisture, free acid, pH, lactone and ash.

___

  • ANONYMOUS , (TSE 3036), 1990. Türk Standartları Enstitüsü, Ankara, Turkey.
  • ANONYMOUS , 1997. Devlet istatistik Enstitüsü(DİE), Ankara, Turkey.
  • ANONYMOUS , A O AC. 1990. Methods of Analysis (15th ed.) Association of Official Analytical Chemists, Washington, DC,USA.
  • BOGDANOV, S. 1981. Determination of honey protein with coomassie brilliant blue Q 250. Mitt. Geb. Lebensm. u. Hgy.72,411-417.
  • BOZKURT, M. and AYDOGAN, A. 1986. Research on the chemical composition of honey at different regions of Turkey.Turkish Bull, of Hyg. and Experimen. Biol. 43(1) 1-22.
  • CRANE,E.1975. Honey. A Comprehensive survey. International Bee Research Association, Heinemann.London. UK.
  • CROFT, L.R.; MISTRY, R. P. and WASHINGTON, R.J.1986. In electropholesls'86 pp. 338 - 389. VCH publishers, Deerfieldbearch.F1.
  • DOLINSEK, B. 1981. Electrophoretic study of proteins in honey and products with pollen. Zborn. Bioteh. Fakul. Univ. v Ljubljani 36, 303-308.
  • DONER, L.W.1977.The sugars of honey: a review. J.Scl.Food Agric. 28, 443-456
  • GUPTA, J.K.; KAUSHIK, R. and JOSHI, V. K. 1992. Influence of different treatments, storage temperature and period on some physico-chemical characteristics and sensory qualities of Indian honey. J.Fd.Sci.Technol. 29(2), 84-87.
  • LAEMMLI, U.K.,1970 Cleage of structural proteins during in assembly of the head of Bacteriofage T4. Nature 227,680-685.
  • LEE, C.Y.; SMITH, N. L; KİME, R. W. and MORSE, R. A. 1985. Source of honey protein responsible for apple juice clarification. J. Apic. Res. 24(3),190-194
  • MARSHALL, T. and WILLIAMS, K. M. 1987. Electrophoresis of honey: Characterization of trace proteins from a complex biological matrix by silver staining. Anal. Biochem. 167, 301-303.
  • RODRIGUEZ-OTERO, J.L.; PASEIRO, P.; SIMAL, J. and CEPEDA, A.1994. Mineral content of the honeys produced in Galicia. Food Chem. 9,169-171
  • SANCHO, M.T.; MUNIATEGUI, S.; HUIDOBRO, J.F. and SIMAL, J. 1992. Aging of honey. J. Agric. Food Chem. 40,134-138.
  • SINGH, N.; SINGH, S.; BAWA, A.S. and SEKHON, K. S. 1988. Honey-its food uses. Indian Food Packer. 42,15-25.
  • WHITE, J. W. Jr; RUDYJ, O. N. 1978. The protein content of honey. J. Apic. Res. 17(4), 234-238.