Ali Doğan DURSUN, Mustafa ARSLAN, Ayşegül KÜÇÜK, Faruk METİN ÇOMU, Dilek ERER, Abdullah ÖZER, Hakan BOYUNAĞA, Tolga TATAR, Yücel POLAT, Mehmet KİRİŞÇİ, Hakan KARTAL

The Effect of Picroside-2 on Erythrocyte Deformability and Lipid Peroxidation in StreptozotocinInduced Diabetic Rats Subjected to Left Anterior Descending Artery-Ischaemia Reperfusion

Amaç: Diyabetes mellitus (DM), özellikle oksidatif stres seviyelerinde yükselme ile karakterize edilen kronik bir metabolik bozukluktur. İskemi-reperfüzyon (İR) hasarı, doku iskemisi ve reperfüzyonla üretilen hücresel hasara yol açan, bir dizi inflamatuar cevap benzeri olay sonucunda başlar. Eritrosit deformabilitesi ve plazma viskozitesi, organ ve doku perfüzyonu için önemli klinik belirteçlerdir. Son yıllardaki araştırmalar, pikrozid-2'nin antioksidan, nöroprotektif ve antiinflamatuar etkilere sahip olduğunu bulmuştur. Çalışmamızın amacı, streptozosin ile diyabet oluşturulan, sol ön inen (LAD) arter için İR uygulanan ratlarda pikrozid-2'nin eritrosit deformabilitesi ve lipid peroksidasyonu üzerine etkilerini araştırmaktı. Yöntem: Hayvanlar rasgele beş gruba ayrıldı. Grup K, DK (diyabet kontrol grubu) ve DP (diyabet-pikrozid-2 grubu) ratlarında koroner arter oklüzyonu veya reperfüzyon uygulanmadı. Grup DİR'de LAD arterinin bir dalı 45 dakika boyunca kapatıldı, bunu takiben İR hasarına neden olmak için 90 dakika reperfüzyon uygulandı. Grup DİRP'de LAD arterinin bağlanmasından 30 dakika önce 10 mg.kg-1 intraperitoneal (IP) yolu ile pikrozid-2 uygulandı. Serum malondialdehit ve nitrik oksit aktiviteleri, lipid peroksidasyonu ve eritrosit deformabilite indeksi değerlendirildi. Bulgular: Deformabilite indeksi diyabetik ratlarda belirgin şekilde arttı (p

The Effect of Picroside-2 on Erythrocyte Deformability and Lipid Peroxidation in StreptozotocinInduced Diabetic Rats Subjected to Left Anterior Descending Artery-Ischaemia Reperfusion

Aim: Diabetes mellitus (DM) is a chronic metabolic disorder principallycharacterized by an elevation in oxidative stress levels. Ischaemia-reperfusion(IR) injury starts a cascade of events that lead to tissue ischaemia and cellulardamage produced by reperfusion causing an inflammatory like response. Erythrocyte deformability and plasma viscosity are important clinicalimplications for organ and tissue perfusion. Recent studies have found thatpicroside-2 has antioxidant, neuroprotective and anti-inflamatory effects. Theaim of our study was to investigate the effects of picroside-2 on erythrocytedeformability and lipid peroxidation in streptozotocin-induced diabetic ratssubjected to left anterior descending (LAD) artery IR.Methods: The animals were randomly assigned to one of five experimentalgroups. In Group (control) C, DC (diabetes-control group), and DP (diabetespicroside-2group) neither coronary artery occlusion nor reperfusion wereperformed in the control rats. In Group DIR, a branch of the LAD artery wasoccluded for 45 minutes followed by 90 minutes of reperfusion to produce IR.In Group DIRP, picroside-2 was administrated via 10 mg.kg-1 inraperitoneal (IP)route 30 minutes before ligating the LAD artery. Serum malondialdehyde andnitric oxide activities were investigated to document lipid peroxidation anderythrocyte deformability index.Results: Deformability index was notably increased in diabetic rats (p

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