Neslihan COŞKUN AKÇAY, Guleser GOKTAS, Gulistan Sanem SARIBAŞ, Seda Nur AKYOL, Seren Gulsen GURGEN, Candan OZOGUL, Deniz ERDOGAN, Ibrahim Murat HİRFANOĞLU, Sermin SEYHAN

Sisplatin ile İndüklenmiş Uterus Toksisitesinde Asetil L-Karnitinin Koruyucu Etkisinin Araştırılması

Amaç: Bu çalışmanın amacı, asetil L-karnitinin, sisplatinin neden olduğu uterus hasarına karşı profilaktik etkilerini araştırmaktır. Yöntem: Yirmi dört adet Wistar albino cinsi dişi sıçanlar dört gruba ayrıldı: 1. gruba (kontrol) salin uygulaması yapıldı; 2. gruba asetil L-karnitin uygulaması yapıldı; 3. gruba sisplatin uygulaması yapıldı; 4. grup ise sisplatinin intraperitoneal enjeksiyonu öncesinde asetil L-karnitin ile ön tedaviye tabi tutuldu. Sisplatin enjeksiyonundan 72 saat sonra uterus dokusu çıkarıldı. Histolojik ve immünohistokimyasal incelemeler yapıldı. Bulgular: TUNEL ve kaspaz pozitif hücre sayılarının, diğer gruplara göre 3. grupta endometriyal epitelde, subepitelyal bağ dokusunda, endometriyal bezlerde ve stromada arttığını bulduk. Ayrıca 3. Gruptaki ratların uterusunda inflamasyon ve ödem gözlenmiştir. Sonuç: Asetil L-karnitin ön tedavi uygulamasının, sisplatin kaynaklı uterus histolojisinin değişikliği üzerinde koruyucu etkisi olduğu sonucuna vardık.

Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity

Objective: The aim of the study was to investigate the prophylactic effects ofacetyl L-carnitine against to uterus induced by cisplatin.Methods: Twenty-four female Wistar albino rats were divided into four groups:group I (control) was administered with saline; group II was administered withacetyl L-carnitine; group III was administered with cisplatin; group IV waspretreated with acetyl L-carnitine before cisplatin intraperitoneal injection. After72h of cisplatin injection uterine tissue was removed. Histological andimmunohistochemical investigations were performed, respectively.Results: We found that the number of TUNEL and caspases positive cells wereincreased in the endometrial epithelium, subepithelial connective tissue,endometrial glands and stroma in group III compare to the other groups.Furthermore inflammation and edema were observed in uterus of rats in groupIII.Conclusion: We can concluded that pretreatment of acetyl L-carnitineadministration has protective effect on histological alteration of uterus causedby cisplatin.

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