Satureja Khuzestanica’nın Antimikrobiyal, Antioksidant, Sitotoksik ve Apoptotik Aktivitesi
Amaç: Antimikrobiyal etki gösteren doğal ürünler, mikroorganizmaların direncine neden olan sentetik antibiyotiklerin kullanımını ortadan kaldırmak için araştırılmaktadır. Bu çalışmada, Satureja khuzestanica bitkisinin antibakteriyel, antioksidan aktivitesi ve çeşitli kanser hücre hatlarında sitotoksik ve apoptotik etkisi araştırılmıştır. Yöntem: Tek tek bileşenler, literatürden bilinen bileşiklere atıfta bulundukları özdeş alıkonma endeksleri ile tanımlanmıştır. S. khuzestanica'nın (25 ve 50 mg / mL) metanol ve etanol özütünün dört Gram-pozitif ve dört Gram-negatif bakteri üzerindeki etkisi belirlendi. Bitki özütlerinin 2,2-difenil-l-pikrilhidrazil (DPPH) radikaline karşı antioksidan aktiviteleri hesaplandı. Özütlerin plazmid DNA üzerine etkisi agaroz jel elektroforezi ile analiz edildi. Ayrıca, özütlerin MCF-7, DLD-1, osteosarkom ve fibroblast hücre hatları üzerinde üzerinde sitotoksik aktiviteleri araştırıldı. Son olarak, gerçek zamanlı PZR ile özütlerin kaspaz-3, Bax ve Bcl-2'nin gen ifadesi üzerine etkisi araştırıldı. Bulgular: Bizim sonuçlarımız, her iki özütünün de hem Gram-negatif (Escherichia coli ATCC 35218) hem de pozitif (Bacillus subtilis ATCC 6633) bakterilere karşı iyi bir inhibitör aktivite gösterdiğini ortaya çıkarmıştır. Ayrıca, S. khuzestanica'nın metanol özütünü kuvvetli bir antioksidan aktivitesine sahip olduğu bulunmuştur (IC50 = 37.0 ± 0.3 μg / mL). Özütler plazmid DNA üzerinde güçlü bir etki göstermiştir. S. khuzestanica'nın metanol özütü konsantrasyon bağımlı güçlü sitotoksik aktivite göstermiştir. Son olarak, IC50, 47.00a ± 1.21μg / mL olarak hesaplanmış, etanol ve su özütü MCF-7 hücre hattında apoptotik etki göstermiştir. Sonuç: MCF-7 en hassas hücre hattı olarak tespit edilmiştir. Meme kanserine karşı potansiyel antikanser ajan olarak ileri çalışmalar yapılması önerilmektedir.
Antimicrobial, antioxidant, cytotoxic and apoptotic activities of Satureja khuzestanica
Objective: Natural products with antimicrobial effect are currentlyinvestigated in order to eliminate the use of synthetic antibiotics that causethe resistance of microorganisms. This study determines the antibacterial,antioxidant activity, and also the cytotoxic and apoptotic effect of Saturejakhuzestanica on various cancer cell lines.Materials and methods: The individual constituents were identified by theiridentical retention indices referring to known compounds from the literature.Methanol and ethanol extracts of S. khuzestanica (25 and 50 mg/mL) werescreened against four Gram-positive and four Gram-negative bacteria. Theplant extracts were tested for their antioxidant activity against 2,2-diphenyl1-picrylhydrazyl(DPPH) radical. The interaction between the extracts andplasmid DNA were analyzed by agarose gel electrophoresis. In addition, thecytotoxic activity of the extracts was evaluated on MCF-7, DLD-1,osteosarcoma, and fibroblast cell line. Finally, gene expression of caspase-3,Bax and Bcl-2 were investigated by real-time PCR.Results: Our result indicated that both extracts showed good inhibitoryactivity against both Gram-negative (Escherichia coli ATCC 35218) and positive(Bacillus subtilis ATCC 6633) bacteria. In addition, the methanol extract of S.khuzestanica had strong antioxidant activity (IC50=37.0±0.3 μg/mL). Theextracts showed a strong effect on plasmid DNA. The methanol extract of S.khuzestanica showed a good concentration-dependent cytotoxicity. Finally,IC50=47.00a±1.21μg/mL, ethanol, and water extract had apoptotic effect inMCF-7 cell line.Discussion: MCF-7 was detected as the most sensitive cell line thereforefurther studies should be done on this plant extract as a potential anticanceragent against breast cancer.
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