Najeeb Mohammed HUSSEİN, Ban Hamid KHALAF, Mustafa Nadhim OWAİD

Pseudomonas Aeruginosa ve Ginkgo Biloba, Ginkgoaceae Sulu Özü Lipopolisakkaritlerin Balb/c Farelerde Hücresel Bağışıklık Yanıtı Üzerindeki Etkileri

Bu çalışmada, Pseudomonas aeruginosa IAN5 izolatının lipopolisakkaridleri (LPS) ve Ginkgo biloba yapraklarının sulu özütü, Balb/c farelerde (in vivo) hücresel bağışıklığı araştırmak için kullanıldı. Bu çalışmanın gerçekleştirilmesinde bazı parametreler kullanıldı; polimorfonükleer nötrofil (PMN'ler) yüzdeleri, 30, 60, 90 ve 120 dakika gibi farklı zaman aralıklarında PMN'lerin fagositoz katsayısı, içerlerinde Formazan granül oluşumu ve göç önleme faktörü (MIF). Farelerin LPS antijeni ile immünizasyonu, gecikmiş tip aşırı duyarlılığı etkilemiş, Candida sp.deki PMN aktivitesini atırmış, NBT'yi azaltmış ve PMN'lerin göçünü inhibe etmiştir. Bağışıklama, PMN'lerin makrofajlarını etkilemedi. LPS ve Ginkgo biloba ekstresi ile enjekte edilen tedavi, LPS ve kontrol tedavileri ile karşılaştırıldığında en iyi sonuçları (p

Effects of Lipopolysaccharides of Pseudomonas Aeruginosa and Aqueous Extract of Ginkgo Biloba, Ginkgoaceae, on Cellular Immune Response in Mice Balb/c

In this study, lipopolysaccharides (LPS) of Pseudomonas aeruginosa IAN5isolate and aqueous extract of Ginkgo biloba leaves were used to investigatethe cellular immunity in mice Balb/c (in vivo). Some parameters were used toachieve this study, are percentages of polymorphonuclear neutrophils(PMNs), phagocytosis coefficient of PMNs at different time periods 30, 60, 90and 120 minutes, Formazan granules formation in them, and migrationinhibition factor (MIF). The immunization of mice with the LPS antigenaffected delay-type hypersensitivity and increased activity of PMNs in Candidasp. and reduced NBT, while inhibited migration of PMNs. The immunizationdid not affect the macrophages of PMNs. The injected treatment with LPS andGinkgo biloba extract showed the best results significantly (p

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