Mehmet TUZCU, Cemal ORHAN, James Richard KOMOROWSKİ, Kazım ŞAHİN, Hafize TELÇEKEN, Nurhan ŞAHİN

Yüksek Yağlı Diyet ile Beslenen Sıçanlarda Krom Histidinatın Farklı İzomerlerinin Pankreas GLUT-2, IRS-1 ve GLP-1R Üzerine Etkileri

Krom (Cr), insülin aktivitesi için esansiyel bir iz elementtir, insülin reseptörüne bağlanır,karbonhidrat ve lipit metabolizmasının düzenlenmesi de dahil olmak üzere birçok fonksiyonu vebelki de tüm fonksiyonlarında insülinin etkisini güçlendirir. Bu çalışmanın amacı, yüksek yağlı diyet(YYD) ile beslenen sıçanlarda, krom histidinatın (CrHis) üç farklı izomerinin, pankreas glikoztaşıyıcı protein 2 (GLUT-2), insülin reseptör substrat (IRS-1) ve glukagon benzeri peptid 1 reseptör(GLP-1R) protein düzeyleri üzerindeki etkilerini araştırmaktır. Çalışmada, 42 adet Sprague-Dawleyırkı sıçan rastgele altı gruba (n=7) ayrıldı. Sıçanlar 1:standart diyet ile beslenen (Kontrol); 2: YYDile beslenen; 3: YYD ile CrHis saflaştırılmış izomer 1 (CrHis1); 4: YYD ile CrHis ile saflaştırılmışizomer 2 (CrHis2); 5: CrHis ile saflaştırılmış izomer 3 (CrHis3); 6: YYD ile üç izomer CrHis(CrHisM) karışımı ile beslenen şeklinde gruplandırıldı. CrHis izomerleri farklı kaynaklardansağlanmasına karşın sıçanlara aynı miktarda Cr (10 µg elementel Cr/kg CA/gün) verildi. Sıçanlar,insülin direncini indüklemek için yüksek yağlı bir diyetle beslendi. CrHis izomerlerinin uygulanması,YYD ile artan pankreas insülin düzeylerini iyileştirdi. CrHis izomer gruplarındaki insülin seviyeleriYYD grubuna göre anlamlı derecede düşük bulundu (P

The Effects of Different Isomers of the Chromium Histidinate on Pancreas GLUT2, GLP-1R and IRS-1 in Rats Fed High-Fat Diet

Chromium (Cr) is an essential trace element for optimal insulin activity. It binds to the insulin receptor and potentiates many, and perhaps all, of its functions including the regulation of carbohydrate and lipid metabolism. The objective of this study was to investigate the effects of novel purified isomers of chromium histidinate (CrHis on protein levels of pancreatic glucose transporter protein 2 (GLUT-2), insulin receptor substrate (IRS-1) and glucagon-like peptide 1 receptor (GLP-1R). Forty-two Sprague–Dawley rats allocated to six groups (n=7). The rats were fed either 1: a standard diet (Control) or 2: a high-fat diet (HFD) or 3: a HFD with CrHis purified isomer 1 (CrHis1) or 4: a HFD with CrHis purified isomer 2 (CrHis2) or 5: a HFD with CrHis purified isomer 3 (CrHis3) or 6: a HFD with a mixture of three isomers of CrHis (CrHisM). Rats were fed the same amount of Cr (10 µg elemental Cr/kg BW/day), but from different sources of CrHis isomers. Rats fed a high-fat diet to induce insulin resistance. The administration of CrHis isomers showed an improvement in the HFD-enhanced pancreatic insulin levels. The insulin levels in the CrHis isomer groups were measured significantly lower than the HFD group (P

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