Hatice ERÖKSÜZ, Abdullah ASLAN, Servet KILIÇ, Kadri KULUALP, Yesari ERÖKSÜZ

Kuru Göz Fare Modelinde Sığır Amniyotik Sıvısının Terapötik Etkisinin Bazı Klinik ve Histopatolojik Parametreler Yönünden Değerlendirilmesi

Bu çalışmada, kuru göz (KG) fare modelinde sığır amniyotik sıvısının (SAS) 4 farklıkonsantrasyonunun terapötik etkisinin bazı klinik ve histopatolojik parametreler yönündenaraştırılması amaçlandı. Çalışmanın materyalini rastgele ve eşit sayıda 6 gruba ayrılan 60 adetBALB/c ırkı dişi fare oluşturdu. Kontrol grubu hariç diğer 5 grubu oluşturan deneklerin her ikigözüne KG modeli oluşturmak amacıyla 14 gün süreyle günde 2 defa %0.2’lik BEK (benzalkonyumklorid)’den 5 µL damlatıldı. 15 ile 30. günler arasında ise II. gruba (KG grubu) %0.9’luk izotonikserum fizyolojik, III. gruba %20 SAS, IV. gruba %35 SAS, V. gruba %50 SAS, VI. gruba ise%100’lük SAS’tan günde 3 defa 5 µL damlatıldı. Tüm gruplar, çalışma boyunca göz kırpma sayısıve konjunktival goblet hücre sayısı; çalışma sonunda ise histopatolojik olarak korneal epitelyalkalınlık, korneal damarlaşma ve yangısal hücre sayısı parametreleri yönünden değerlendirildi.Çalışma sonunda en etkili sonuçlar, göz kırpma sayısı için %35, %50 ve %100 SAS, konjunktivalgoblet hücre sayısı için %35 SAS gruplarında alındı. KG grubu deneklerin korneal epitelyaltabakalarının kontrol grubuna göre belirgin şekilde inceldiği, korneal damarlaşma ve yangısal hücresayılarının ise arttığı saptandı. Bu parametrelere göre en etkili sonuçlar %35 ve %50 SASgruplarında alındı. Bu sonuçlar, SAS’ın orta ve yüksek konsantrasyonlarının KG’nin klinik vehücresel belirtileri üzerine faydalı etkileri olduğunu göstermektedir.

Evaluation of the Therapeutic Effect of Bovine Amniotic Fluid in Dry Eye Mouse Model in Terms of Some Clinical and Histopathological Parameters

In this study, we aimed to investigate the therapeutic effect of 4 different concentrations of bovine amniotic fluid (BAF) in a dry eye (DE) mouse model in terms of some clinical and histological parameters. The material of the study consisted of 60 BALB/c female mice that were randomly divided into 6 groups with equal numbers. In both eyes of the other 5 groups except the control were instilled 5 µL 0.2% BAC BAC (benzalkonium chloride),, twice a day for 14 days in order to form a DE model. Between 15th and 30th days, group 2 (DE group) received 5 µL 0.9% isotonic saline, group 3 20% BAF, group 4 35% BAF, group 5 50% BAF and group 6 100% BAF, three times a day. All groups were evaluated regarding blink rate and conjunctival goblet cell count during the study. Corneal epithelial thickness, corneal vascularization and inflammatory cells count were assessed by histopathological examination in all groups at the end of study. At the end of the study, the most effective results were obtained in 35%, 50% and 100% BAF for the blink rate; and in 35% BAF groups for conjunctival goblet cell count. It was determined that the corneal epithelial layers of the DE group subjects were significantly thinner and corneal vascularization and inflammatory cell count were increased. According to these parameters, the most effective results were obtained in 35% and 50% BAF groups. These results suggest that moderate and high concentrations of BAF have beneficial effects on clinical and cellular symptoms of DE.

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