Tekelerde İki Farklı Sulandırıcı ile Sulandırılan Spermaya Değişik Oranlarda Bal İlavesinin Kısa Süreli Saklamaya Etkisi

Çalışmada, yağsız süt ve Tris-yumurta sarısı sulandırıcıları ile sulandırılan teke spermasına farklıoranlarda bal ilavesinin kısa süreli saklamada spermatolojik özelliklere ve yaşam süresine etkisiincelendi. Sperma 2 ergin tekeden elektroejakulatör ile haftada 2 kez alındı ve + 4-6 °C’desaklanan sperma örnekleri 12 saat aralıklarla değerlendirildi. Motilite, süt kontrol grubunda 24. saatve 60. saat arasında bal içeren gruplardan, Tris kontrol grubunda ise 48. saate kadar bal içerengruplardan yüksek bulundu. Hipo osmotic swelling (HOS) test değeri, süt kontrol grubunda 12.ve 24. saatlerde %2.5 ve %5 bal ilaveli gruplardan yüksek, %1 bal ilaveli grup ile benzer, 36 ve 60.saatler arasında kontrol grubu bal ilaveli gruplardan yüksekti. HOS test değerleri, Tris kontrolgrubunda 48. saate kadar yüksek, 60. saatte %1 bal ilaveli gruba benzerdi. Anormal spermatozoonoranı, Tris kontrol grubunda 48. saate kadar %1 bal içeren gruptan, 48. saatte ise % 1 ve 5 balilaveli gruptan düşük bulundu. Ölü/canlı spermatozoon oranı, süt kontrol grubunda 0. saatte %5 balilaveli gruptan, 12. saatte %2.5 ve %5 bal ilaveli gruptan, 24. saat ile 72. saatler arasında ise balilaveli tüm gruplardan düşük, Tris kontrol grubu 60. saate kadar bal ilaveli gruplardan, 72. saatte isesadece %5 bal ilaveli gruptan düşük bulundu.Sonuç olarak, teke spermasının kısa süreli saklanmasında, Tris ve süt sulandırıcılı kontrolgruplarının spermatolojik kalite ve yaşam süresi bakımından deneme gruplarına göre daha iyiolduğu, gruplar arası değerlendirmede Tris sulandırıcısının üstün olduğu ve sulandırıcılara balilavelerinin spermatolojik özelliklerde herhangi bir ilerleme sağlamadığı saptandı.

Effect of Different Rates of Honey Supplementation on Short Term Storage Semen Diluted with Two Different Diluents in Male Goats

In theise study, the effect of addition of honey at different ratios to goat semen diluted with skim milk and tris+egg yolk extenders on spermatological characteristics and lifespan during short term storage was investigated. The semen samples stored at 4-6°C. The motility between 24th and 60th hour in milk control group and up to 48th hour in tris control group was found higher than all honey groups. The HOS test value was similar in milk control with 1% honey group and was higher than other groups at 12nd and 24th hour. Between 36th and 60th hour, the control was higher than other groups. The HOS test values were high in tris control group up to 48th hour than all honey groups but similar to 1% honey group at 60th hour. The abnormal spermatozoon ratio in Tris control was lower than 1% honey group until 48th, 1% and 5% honey group at 48th hour. The dead/live spermatozoon ratio was lower in the milk control than in 5% honey group at 0st, 2.5% and 5% honey group at 12nd, between 24th and 72nd hour all trial groups. Tris control was lower than other groups up to 60th, only 5% honey group at 72nd hour. As a result, the control groups of tris and milk extenders were better than trial groups during short term storage with regard to spermatological quality and lifespan. It was concluded that tris extender was superior considering the intergroup comparisons and honey didn’t provide any improvement in the spermatological features.

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