Fatih AVDATEK, Mustafa GÜNDOĞAN, Deniz YENİ

Merinos Irkı Koçlarda Spermaya Katılan Krosinin Dondurma ve Çözdürme Sonrası Spermatolojik Parametreler, Oksidatif Stres ve DNA Hasarı Üzerine Etkileri

Bu çalışmanın amacı koç sperma sulandırıcısına ilave edilen farklı dozlardaki krosinin çözüm sonuspermatolojik parametreler, oksidatif stres ve DNA hasarı üzerine etkilerini araştırmaktır. Ejakulatlarbeş baş Merinos koçtan haftada bir kez suni vajen yardımıyla toplandı ve bu işlem altı keretekrarlandı. Ejakulatlar ml’de 150x106 spermatozoon olacak şekilde antioksidan içermeyen (kontrol)ve antioksidan içeren (0.5 mM, 1 mM ve 2 mM) sulandırıcılar ile dört bölüme ayrıldı. Sulandırılanörnekler 0.25 mL’lik payetlerde 5 ⁰ C’de 3 saat ekilibrasyona tabi tutulduktan sonra sıvı azotbuharında donduruldu. Çözüm sonu subjektif motilite açısından kontrol grubuna göre 0.5 mM ve 1mM gruplarda en yüksek değerler elde edildi (P

Effects of Crocin Supplementation on Spermatological Parameters, Oxidative Stress and DNA Damage After Freezing-Thawing Process in Merino Ram Semen

The aim of this study was to investigate the effects of different doses of crocin added to the extender on some spermatological parameters, oxidative stress, and DNA damage in thawed ram semen. Ejaculates were collected from five Merino rams using an artificial vagina once a week and this process was repeated six times. Ejaculates were split into four aliquots and diluted to a final concentration of 150x106 spermatozoa/ml with the base extender containing crocin (0.5, 1 and 2 mM) and no additive (control). All samples were cooled to 5°C and equilibrated for 3 h then were loaded into 0.25 mL straws and frozen using a liquid nitrogen vapour and plunged into the liquid nitrogen at –196°C. In the thawed semen according to subjective motility respectively 0,5 and 1mM crocin doses group indicated the highest value (P

PDF

___

Trounson AO. Cryopresrevation. Brit Med Bull 1990; 147: 427-433.
Gordon IR. Artificial inseminatiom In: Gordon IR (Editor). Reproductive Technologies in Farm Animals. USA: Cambridge, MA, 2005.
Maxvell WMC, Watson PF. Recent progress in the preservation of ram semen. Anim Reprod Sci 1996; 42: 55-65.
Peris SI, Bilodeau JF, Dufour M, Bailey JL. Impact of cryopreservation and reactive oxygen species on DNA integrity, lipit peroxidation, and functional parameters in ram sperm. Mol Reprod Dev 2007; 74: 878-892.
Bilodeau JF, Blanchette S, Gagnon C, Sirard MA. Thiols prevent H2O2-mediated loss of sperm motility in cryopreserved bull semen. Theriogenology 2001; 56: 275- 286.
Chatterjee S, De Lamirande E, Gagnon C. Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: protection by oxidized glutathione. Mol Reprod Dev 2001; 60: 498-506.
Mazur P, Katkoov II, Katkova N, Critser JK. The enhancement of the ability of mouse sperm to survive freezing and thawing by the use of high concentrations of glycerol and the presence of an Escherichia coli membrane preparation (Oxyrase) to lower the oxygen concentration. Cryobiol 2000; 40: 187-209.
Papandreou MA, Tsachaki M, Efthimiopoulos S, et al. Memory enhancing effects of saffron in aged mice are correlated with antioxidant protection. Behav Brain Res 2011; 219: 197-204.
Mansoori P, Akhondzadeh S, Raisi F, et al. A randomized, double-blind, placebo-controlled study of safety of the adjunctive saffron on sexual dysfunction induced by a selective serotonin reuptake inhibitor. J Med Plants 2011; 10: 121-130.
Abdullaev FI. Cancer chemopreventive and tumoricidal properties of saffron (Crocus sativus L.). Exp Biol Med 2002; 227: 20-25.
Assimopoulou AN, Sinakos Z, Papageorgiou VP. Radical scavenging activity of Crocus sativus L. extract and its bioactive constituents. Phytother Res 2005; 19: 997-1000.
Tsantarliotou MP, Poutahidis T, Markala D, et al. Crocetin ameliorates endotoxin-induced disseminated intravascular coagulation in rabbits. Blood Coag Fibrinol 2013; 24: 305- 10.
Heidary M, Nejadi JR, Delfan B, et al. Effect of saffron on semen parameters of infertile men. Urol J 2008; 5:55-59.
Mardani M, Vaez A, Razavi S. Effect of saffron on rat sperm chromatin integrity. Iran J Reprod Med 2014; 12: 343-350.
Sapanidou V, Taitzoglou I, Tsakmakidis I, et al. Antioxidant effect of crocin on bovine sperm quality and in vitro fertilization. Theriogenology 2015; 84: 1273-1282.
Edge R, Mcgarvey DJ, Truscott TG. The carotenoids as antioxidants: a review. J Phytochem Phytobiol 1997; 41: 189-200
Ochiai T, Ohno SH, Soedo SH, et al. Crocin prevents the death of rat pheochromyctoma (PC-12) cells by its antioxidant effect stonger than those of α-tocopherol. Neurosci Lett 2004; 362: 61-64.
Watson PF. Use of a Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa. Vet Rec 1975; 97: 12-15.
Gündoğan M, Avdatek F, Yeni D. Effect of extenders on motility, morphology and osmotic resistance parameters of ram sperm during liquid storage. Revue Méd Vét 2011; 162: 546-551.
Ostling O, Johanson KJ. Microelectrophoretic study of radiation-induced DNA damage in individual mammalian cells. Biochem Biophys Res Commun 1984; 123: 291-298. 21. double-strand breaks and apoptosis in human sperm. Fertil. Steril 2003; 80: 1420-1430.
Erel Ö. A novel automated method to measure total antioxidant response against potent free radical reactions, Clin Biochem 2004; 37: 112-119.
Erel Ö. A new automated colorimetric method for measuring total oxidant status, Clin Biochem 2005; 38: 1103-1111.
Mata-Campuzano M, Álvarez-Rodríguez M, Álvarez M, et al. Post-thawing quality and incubation resilience of cryopreserved ram spermatozoa are affected by antioxidant supplementation and choice of extender. Theriogenology 2015; 83: 520-528.
Dominguez-Rebolledo AE, Fernandez-Santos MR, Bisbal A, et al Improving the effect of incubation and oxidative stress on thawed spermatozoa from red deer by using different antioxidant treatments. Reprod Fertil Dev 2010; 22: 856-870.
Khazaei Y, Moghbelinejad S. Increasing viability, numbers, and motility of sperm in men with normal spermatogenesis exposed to saffron extract after freezing-thawing process. J Qazvin Univ Med Sci 2017; 21: 12-20.
Asadi MH, Zafari F, Sarveazad A, Abbasi M, et al. Saffron improves epididymal sperm parameters in rats exposed to cadmium. Nephro Urol Mon 2014; 6: e12125.
Safarinejad MR, Shafiei N, Safarinejad S. A prospective double-blind randomized placebo-controlled study of the effect of Saffron (Crocus sativus Linn.) on semen parameters and seminal plasma antioxidant capacity in infertile men with idiopathic oligoasthenoteratozoospermia. Phytother Res 2011; 25: 508-516.
Premkumar K, Thirunavukkarasu C, Abraham S, Santhiya S, Ramesh A. Protective effect of saffron (Crocus sativus L.) aqueous extract against genetic damage induced by anti-tumor agents in mice. Hum Experiment Toxicol 2006; 25: 79-84.
Bakhtiary Z, Shahrooz R, Ahmadi A, Zarei L. Evaluation of antioxidant effects of krosin on sperm quality in cyclophosphamide treated adult mice. Veterinary Research Forum 2014; 5: 213-218.
Tseng TH, Chu CY, Huang JM, Shiow SJ, Wang CJ. Crocetin protects against oxidative damage in rat primary hepatocytes. Cancer Letters 1995; 97: 61-67.