Ayşe KILIÇ, Gökben ÖZBEY, Adile MUZ, Hasan Basri ERTAŞ

Random amplified polymorphic DNA (RAPD) analysis of Escherichia coli ısolated from chickens

Bu çalışmada, colibacillosis şüpheli farklı kanatlı işletmelerinden 48 E.coli suşu izole ve identifiye edildi. Suşların kanlı agara ekimleri yapıldıktan sonra, EMB ve MacConkey agara ekimleri yapıldı.110 örneğin bakteriyel kültürü sonucunda 48 (%13,6)’i E.coli olarak identifiye edildi. Bu bölgedeki E.coli soyları arasındaki genetik farklılıkları tespit etmek için RAPD metodu uygulandı. 48 E.coli izolatından ekstrakte edilen DNA’lar RAPD primer (COL 1) ile çoğaltıldı. RAPD analizinden dokuz farklı profil elde edildi. Bu sonuçlar bu bölgedeki E.coli soyları arasında büyük bir genetik farklılık olduğunu gösterdi. RAPD metodunun E.coli soylarını tiplendirmede yüksek oranda ayırıcı olduğu görüldü. Sonuç olarak bu çalışma; RAPD metodunun hem kolay hem de daha hızlı olmasından ötürü, E.coli’nin moleküler epidemiyolojisinin çalışılmasında, yüksek oranda etkili bir metod olduğunu gösterdi.

Tavuklardan izole edilen Escherichia coli suşlarının random amplified polymorphic DNA (RAPD)analizi ile tiplendirilmesi

In this study, 48 E.coli strains isolated from colibacillosis suspicious hens at different poultry houses were examined. These samples were inoculated on to blood agar and then suspicious colonies were transferred on to EMB and MacConcey agar. Bacterial growth was observed in 110 samples, 48 (13,6%) of which were identified as Escherichia coli (E.coli). E.coli strains were typed by randomly amplified polymorphic DNA (RAPD) to detect the genetic differences between at E.coli strains in this region. Extracted DNA samples from 48 strains of E.coli were amplified by RAPD primer (COL 1). Nine different band profiles were obtained. These results indicated that there were great genetic heterogeneity between E.coli strains in this region. RAPD analysis had the highest discriminatory capacity for typing E.coli isolates. Because of its simplicity and rapidity, RAPD analysis appears to be a highly valuable tool for studying E.coli molecular epidemiology.

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