Oğuz MERHAN, Onur YILMAZ, Kadir BOZUKLUHAN, Gürbüz GÖKÇE

İshalli Buzağılarda Haptoglobin, Seruloplazmin ve Bazı Biyokimyasal Parametre Düzeylerinin Araştırılması

Bu çalışmanın amacı klinik olarak ishalli olan buzağılarda serum haptoglobin, seruloplazmin ve bazı biyokimyasal parametre düzeylerinin ve diagnostik önemlerinin araştırılmasıdır. Bu amaçla çalışmada 25 adet ishalli ve 10 adet sağlıklı buzağı kullanıldı. Hayvanların vena jugularis'inden antikoagulansız tüplere kan örnekleri toplandı. Kan örnekleri santrifüj edilerek serum örnekleri çıkarıldı. Analizler yapılıncaya kadar -20 °C'de saklandı. Serum örnekleri; haptoglobin, seruloplazmin, aspartat aminotransferaz (AST), gama-glutamil transferaz (GGT), üre, kreatinin, total protein ve albümin ölçümlerinde kullanıldı. Yapılan analiz sonucunda ishalli buzağılar ile kontrol grubundaki hayvanlar karşılaştırıldığında haptoglobin, seruloplazmin, AST, GGT, üre, kreatinin, total protein değerlerinin yükseldiği belirlendi. Sonuç olarak; yapılan bu çalışmada ishalli buzağılarda akut faz yanıtın oluştuğu ve bunun sonucu olarak haptoglobin, seruloplazmin sentezinin arttığı ve biyokimyasal parametrelerde önemli değişikliklerin oluştuğu belirlendi.

Investigation on the Levelsof Haptoglobin, Ceruloplasmin and Some Biochemical ParametersLevels in Calves with Diarrhea

The aim of the present study was to determine concentrations of haptoglobin, ceruloplasmin and some biochemical parameter levels and to investigate their diagnostic importance in calves with diarrhea. For this purpose 25 calves with diarrhea were used as working group and 10 healthy cattle as control group. Blood samples were collected from Jugular vein in tubes without anticoagulant. Blood samples were centrifuged and the resulting serum samples were stored at -20 °C until laboratory testing. Samples were used to determine the concentrations of haptoglobin, ceruloplasmin, aspartate transaminase (AST), gamma-glutamyl transferase (GGT), urea, creatinin, total protein and albumin. Results of present study indicated that the concentrations of AST, GGT, urea, creatinin, total protein, haptoglobin and ceruloplasmin were significantly increased in calves with diarrhea. In conclusion; in this study in calves with diarrhea occurs acute phase response and as a result haptoglobin and ceruloplasmin synthesis increased and it was determined that significant changes occurred in the biochemical parameters.

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