Harun KAYA KESİK, Selin GELEN, Sami ŞİMŞEK

Fasciola hepatica'nın Morfometrik ve Moleküler Analizi

Bu çalışma, erişkin Fasciola spp. örneklerinin morfolojik ve morfometrik analizinin yapılması ve moleküler analiz sonuçlarıyla kıyaslanması amacıyla planlanmıştır. Bu amaçla, 20 farklı sığırdan kesim sonrası elde edilen 20 erişkin Fasciolaspp. örneği çalışmaya dahil edilmiştir. Parazitlerin morfometrik analizleri stereo mikroskopta 7 farklı parametre kullanılarak yapılmıştır. Moleküler analiz amacıyla ise gDNA izolasyonunu takiben mt-CO1 gen bölgeleri polimeraz zincir reaksiyonu(PZR)ile çoğaltılmış ve takiben kesin tür tayini için AluIenzimi kullanılarak polimeraz zincirreaksiyonu-restriksiyon bölümünün uzunluk değişkenliği(PZR-RFLP)analizi yapılmıştır. Neticede erişkin parazitlerin morfolojik olarak benzer ölçülerde oldukları ve moleküler olarak da hepsinin F. hepaticaolduğu tespit edilmiştir. Bu çalışma ile F.hepatica'nın morfometrik ve moleküler ayrımı konusuna dikkat çekilmiştir.

Morphometric and Molecular Analysis of Fasciola hepatica

This study was planned for the aim of performing a morphological and morphometric analysis of adult Fasciola spp. and comparing those with results of molecular analysis. Twenty adult Fasciola spp. samples obtained from 20 different cattle after slaughtering were included in the study. Morphometric analysis of parasites was performed using 7 different parameters under stereo microscope. For the molecular analysis, the mt-CO1 gene region was amplified using polymerase chain reaction (PCR) following the gDNA isolation, subsequently for the accurate species identification polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was conducted using Alul enzyme. All adult parasites had similar measurements and also they were detected as F. hepatica. In this study, morphometric and molecular differantiation of F.hepatica has been highlighted.

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