Ali BAYRAKDAR, Mine YAMAN, Berrin TARAKÇI GENÇER

Immunohistochemical localization of some neurotransmitters in mole-rats (Spalax leucodon) intestinal plexus

Bu çalışmanın amacı kör fare (Spalax leucodon) intestinal pleksüslarında kalsitonin gene-related peptid (CGRP), substance P (SP), nöropeptid Y (NPY), vasoactive intestinal peptid (VIP) ve serotonin immunoreaktivitesinin varlığını araştırmaktır. İmmunohistokimyasal incelemeler ince ve kalın bağırsağın ileum ve kolon bölümlerinde yapıldı. CGRP immunoreaktif nöronal yapılar ileum ve kolonun miyenterik pleksüsları içinde enterik gangliyonlar şeklinde belirlendi. SP immunoreaktivitesi bu bağırsak bölümlerinin her iki kas tabakası içinde uzayan sinir telleri ve miyenterik ganglionlar şeklinde ayıtedildi. Ayrıca NPY sinir hücre cisimcikleri ve iplikleri ileum ve kolonun miyenterik pleksüs ve lamina propriyasında mevcuttu. Buna karşın VIP ve serotonin immunoreaktif yapılar ince ve kalın bağırsağın herhangi bir bölümünde tespit edilemedi. Sonuç olarak, bu çalışma kör farenin ince ve kalın bağırsaklarının mukozal ve gangliyoner pleksüslarında farklı nöronal popülasyonların varlığını ortaya koydu.

Kör farenin (Spalax leucodon) intestinal pleksüslarında bazı nörotransmiterlerin immunohistokimyasal lokalizasyonu

The aim of this study was to investigate the presence of calcitonin gene-related peptide (CGRP), substance P (SP), neuropeptide Y (NPY), vasoactive intestinal peptide (VIP) and serotonin immunoreactivity in the intestinal plexus of mole-rats (Spalax leucodon). Immunohistochemical methods were conducted on the ileum and colon of the small and large intestine. CGRP immunoreactive neuronal structures were detected in the form of enteric ganglia within the myenteric plexus of ileum and colon. SP immunoreactivity in both of muscle layer of these intestine regions were identified in the form of running nerve fibers and myenteric ganglia. Also, NPY immunoreactive nerve cell bodies and fibers were present in the lamina propria and myenteric plexus of ileum and colon. However, VIP and serotonin immunoreactive neuronal structures were not detected in any other region of the small and large intestines. In conclusion, this study demonstrated the presence of different neuronal populations in the mucosal and ganglionated plexuses in the small and large intestine of the mole rats.

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