Ali Osman ÇERİBAŞI, Mustafa ÖZKARACA, Songül ÇERİBAŞI, Harun ÖZER

Immunohistochemical detection of Peste des Petits Ruminants (PPR) viral antigens in pneumonic caprine lungs in Elazig region

Bu çalışmada Elazığ yöresindeki pnömonili keçi akciğerlerinde immunohistokimyasal yöntemle küçük ruminant vebası (PPR) viral antijenlerinin varlığı ve yaygınlığının tespiti amaçlanmıştır. Bu amaçla, Elazığ’daki bir mezbahada kesilen, 889 erkek kıl keçisinin akciğerleri muayene edildi. Çalışmada makroskobik olarak 152 olguda (17.10%) pnömoni tespit edildi, pnömonilerde apikal ve kardiyak loplarda lezyon şiddeti değerlendirildiğinde 97’sinde hafif, 39’unda orta, 17’sinde şiddetli konsolidasyon bulunduğu saptandı. Pnömonili 152 akciğer mikroskobik olarak kataral-purulent (41, %26.97), intersitisyel (72, %47.37), fibrinli (34, %22.37), verminöz (2, %1.32), pulmoner adenomatoz (3, %1.97) olarak sınıflandırıldı. PPR viral antijenlere; histopatolojik olarak hafif şiddette konsolidasyona sahip kataral-purulent bronkopnömoni (n=3), orta şiddette konsolidasyona sahip intersitisyel (n=2) ve fibrinli bronkopnömoni (n=2) belirlenen, 7 olguda (%4.61) rastlandı. Viral antijenlere ilişkin immunboyama bronş ve bronşiyol epitelinde daha yoğun olmak üzere lümendeki eksudat içerisinde de tespit edildi. Sonuç olarak etkenin keçi pnömonilerinde önemli etiyolojik ajanlardan biri olabileceği kanısına varılmıştır. Ek olarak, sunulan çalışmada histopatolojik lezyonların nonspesifik olduğu PPR kaynaklı pnömoniler dikkate alındığında immunohistokimyasal yöntemin PPR enfeksiyonlarının teşhisinde ileri laboratuvar metodlarına alternatif bir yöntem olarak kullanılabileceği kanaatine varılmıştır.

Elazığ Yöresinde pnömonili keçi akciğerlerinde küçük ruminant vebası (PPR) viral antijeninin immunohistokimyasal olarak belirlenmesi

The aim of this study was to evaluate presence and distribution of Peste des Petits Ruminants (PPR) infection by immunohistochemistry in goats with pneumonia in the Elazig region. For this purpose, lungs of 889 slaughtered male hair goats were macroscopically analysed for pneumonia lesions. Among the 152 cases of pneumonia, 97 (63.82%) exhibited mild lesions, 39 (25.66%) had moderate lesions and 16 (10.53%) had severe lesions according to the extent of consolidation, and they were classified microscopically as catarrhal-purulent (n=41, 26.97%), interstitial (n=72, 47.37%), fibrinous (n=34, 22.37%), verminous (n=2, 1.32%), and pulmonary adenomatosis (n=3, 1.97%). The PPR antigens were detected in 7 cases three of which had catarrhal-purulent bronchopneumonia with mild consolidation, two had interstitial and the remaining had fibrinous bronchopneumonia with moderate consolidation by using direct immunohistochemistry on paraffine sections. Specific immunostaining related with viral antigens was found more intensely in the bronchi and the bronchiole epithelium and, also cellular exudates in the lumen. These results show that PPR viruses may be regarded as one of the aetiological agents in goat pneumonia. Furthermore, when non-specific histopathological lesions in the pneumonia with PPR origin, as evidenced in the presented study, are considered, the immunohistochemistry can be used as an alternative method to the advanced laboratory methods for diagnosis of PPR infections in goats.

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