Immunohistochemical localization of calbindin-D28k in porcupine (Hystrix cristata) trigeminal ganglion

Oklu kirpi (Hystrix cristata) trigeminal gangliyonunda histolojik olarak ışık mikroskopik yapı, immunohistokimyasal olarak ise calbindin-D28k’nın varlığı ve dağılımı araştırıldı. Çok sayıda calbindin-pozitif nöron gangliyona dağılmıştı. Pozitif hücrelerin hepsi büyük ve orta hacimliydi. Küçük gangliyon nöronları boyanmadı. Küçük, orta ve büyük hacimli olmak üzere 3 tip nöron vardı. Nöronların çoğu küçük satellit hücrelerle kuşatılmıştı. Satellit hücrelerin şekli polimorfal ve poligonaldı.

Oklu kirpi (Hystrix cristata) trigeminal gangliyon’ unda calbindin-D28k’nın iimmunohistokimyasal lokalizasyonu

Histologically light microscopic structure, and immunohistochemically the presence and distribution of calbindin-D28k was investigated in the trigeminal ganglion of porcupine (Hystrix cristata). Many calbindin-immunoreactive neurons were distributed throughout the ganglia. All immunopositive cells were medium to large in size. Small ganglion neurons were not stained. There were three types of neurons as small, medium and large. Most of the neurons were enveloped by small satellite cells. The shapes of satellite cells were polimorphal and polygonal.

PDF

___

1. Andressen C, Blumcke I, Celio MR. Calcium-binding proteins: Selective markers of nerve cells. Cell and Tissue Research 1993; 271: 181-208.
2. Suzuki N, Hardebo JE, Owman C. Origins and pathways of cerebrovascular nerves storing substance P and calcitonin gene-related peptide in rat. Neuroscience 1989; 31: 427- 438.
3. Ichikawa H, Deguchi T, Fujiyoshi Y, et al. Calbindin-D28k immunoreactivity in the trigeminal ganglion neurons and molar tooth pulp of the rat. Brain Research 1996; 715: 71- 78.
4. Krastev D. Satellite cells of trigeminal ganglion. Journal of IMAB-Annual Proceeding (Scientific Papers), Book 1, 2007.
5. Krastev D, Paloff A, Hinova-Palova D, Apostolov A, Ovtscharoff W. Light-microscopic structure of trigeminal ganglion in humans. Journal of IMAB-Annual Proceeding (Scientific Papers), Book 1, 2007.
6. Shimizu T, Suzuki N, Takao M, Koto A, Fukuuchi Y. Calbindin-D28k in cerebrovascular extrinsic innervation system of the rat. Autonomic Neuroscience: Basic and Clinical, 2000; 84: 130-139.
7. Ichikawa H, Sugimoto T Co-expression of VRL-1 and calbindin D-28k in the rat sensory ganglia. Brain Research 2002; 924: 109-112.
8. Shin NS, Lee I.S, Yoon YS, Lee HS. Immunohistochemical localization of substance P, calcitonin gene-related peptide, galanin and calcium-binding proteins in trigeminal ganglia of goat (Capra hircus). Anatomia Histologia Embryologia 2003; 32, 310-315.
9. Lazarov N, Dandov A, Stoyanova I, Chouchkov C. Calcium-binding proteins in the mesencephalic trigeminal nucleus of the cat. Archives of Physiology and Biochemistry, 1998; 106(5): 370-377.
10. Kuramoto H, Furness JB, Gibbins IL. Calbindin immunoreactivity in sensory and autonomic ganglia in the guinea pig. Neuroscience Letters 1990; 115: 68-73.
11. Ichikawa H, Yamaai T, Jacobowitz DM, et al. Effect of Brn- 3a deficiency on parvalbumin-, calbindin D-28k-, calretininand calcitonin gene-related peptide-immunoreactive primary sensory neurons in the trigeminal ganglion. Neuroscience 2002; 113(3): 537-546.
12. Polak, JM, Van Noorden S. Introduction to immunocytochemistry. 2nd Edition, Oxford, UK: BIOS Scientific Publishers, 1997.
13. Shu S, Ju G, Fan L. The glucose oxidase-DAB-nickel method in peroxidase histochemistry of the nervous system. Neuroscience Letters 1988; 85: 169-171.
14. Lieberman AR. Sensory ganglia. In: Landon DN. (Editor), The Peripheral Nerve. New York: Wiley 1976: 188-278.
15. Kummer W, Heym C Correlation of neuronal size and peptide immunoreactivity in the guinea pig trigeminal ganglion. Cell and Tissue Research 1986; 245: 657-665.
16. Kuwayama Y, Stone RA Neuropeptide immunoreactivity of pericellular baskets in the guinea pig trigeminal ganglion. Neuroscience Letters 1986; 64: 169-172.
17. Beaver DL, Moses HL, Ganote CE. Electron microscopy of the trigeminal ganglion. III. Trigeminal neuralgia. Archives Pathology 1965; 79: 571-582.