Farelerde modifiye evaporatif kuru göz (KG) modelinin bazı klinik parametreler yönünden değerlendirilmesi

Kuru göz sendromu (KGS); oküler yüzeye hasar verme potansiyeline sahip olan yangı, gözyaşı film osmolaritesinin artışı, instabilitesi ve görme bozukluğu ile karakterize multifaktöriyel bir hastalıktır. KGS’li olguların yaklaşık %40’ı evaporatif stres faktörleri tarafından oluşmaktadır. Bundan dolayı son dönemlerde birçok çalışma, evaporatif kuru göz modeli üzerine yoğunlaşmaktadır. Mevcut çalışmada, bir kuru göz kabini (KGK) (relatif nemi (RN) %30’dan düşük, sıcaklığı 21-25°C) tasarlanmıştır. 14 adet 8 haftalık BALB-C ırkı dişi fare; deney grubu 3 bölmeli KGK’ya, kontrol grubu ise oda şartlarındaki (RN %50-80, sıcaklığı 21-23°C) normal kafeslere yerleştirilmek üzere 2 eşit gruba ayrılmıştır. Göz kırpma sayısı, fenol kırmızısı pamuk ipliği (FKPT) ve gözyaşı kırılma zamanı (GKZ) testi gibi parametreler 2 hafta aralıklarla (0, 2, 4, 6) değerlendirilmiştir. Deney grubu verilerinin ölçüm zamanlarına göre değerlendirilmesinde; 0. haftaya göre göz kırpma sayı ortalamalarının 4 ve 6. haftalarda arttığı, FKPT ve GKZ ortalamalarının ise 2, 4 ve 6. haftalarda sürekli olarak azaldığı kaydedilmiştir. Kontrol ve deney grupları arasında çalışmanın 0. haftasında tüm parametreler yönünden istatistiksel açıdan önemsiz olan fark (P>0.05); 2, 4 ve 6. haftalarda göz kırpma sayısı ile GKZ, 4 ve 6. haftalarda ise FKPT yönünden önemli bulunmuştur (P

Evaluation of modified dry eye (DE) model in mice with different clinical parameters

Dry eye syndrome (DES) is a multifactorial disorder characterized with inflammation, tear film hyperosmolarity and instability and visual impairment, with a potential to induce ocular surface damage. About 40 % of DES cases are induced by evaporative stres factors. Thus, many studies recently concentrated on evaporative dry eye models. In the present study, a dry eye cabinet (DEC) (relative humidity (RH) 0.05) in terms of all parameters on week 0 between control and experimental groups, was found significant (P

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