Gebe olmayan izole sığır uterus kontraksiyonları üzerine ceftiofur'un etkileri

Bu çal ı ş mada üçüncü ku ş ak bir sefalosporin olan ceftiofurun izole gebe olmayan inek ve düvemiyometriyumunun kendili ğ inden kas ı lmaları üzerine etkileri ara ş tı rı ld ı .Siklusun foliküler döneminde bulunan inek ve düvelerden al ı nan miyometriyal ş eritler, %95 oksijen%5 karbondioksit içeren gaz ile sürekli gazlanan, 38 ° Cdeki ve pHs ı 7.4 olan, 5 mL Krebssolüsyonu bulunan izole organ banyosuna as ı ld ı . Doksan dakikal ı k gerime uyum süresi sonunda,düzenli kendili ğ inden kas ı lma gösteren ş eritlerin kas ı lmaları , izometrik güç çevirgeci kullan ı larak 10dakika süre ile kaydedildi ve bu veriler kontrol olarak kullan ı ld ı . Daha sonra ş eritlerin bulundu ğ ubanyo s ı vı s ı na 500 µM, kümülatif 1000 µM ve 2000 µM dozları nda ceftiofur ilave edildi (n=16). Herdoz eklemesinden sonra kas ı lmalar 10 dakika süreyle kaydedildi. Ceftiofurun 10 dakikal ı kperiyotta, kendili ğ inden kas ı lmaları n s ı kl ı k, büyüklük ve e ğ ri altı nda kalan alan (EAKA)parametreleri üzerine etkisi de ğ erlendirildi. Verilerin istatistiksel analizinde Wilcoxon Signed RanksTesti ve Pearson Korrelasyon Analiz Testinden yararlan ı ld ı .İzole organ banyosuna eklenen tüm dozlarda ceftiofurun kas ı lmaları n s ı kl ı ğ ı n ı azalttı ğ ı (P0.05), kümülatif 2000 µM sonras ı artı ş ı n önemli oldu ğ u(P

Effects of ceftiofur on conctractions of uterine isolated from non-pregnant cows

This study investigated the effects of ceftiofur, a third -generation cephalosporin on spontaneouscontractility of the myometrium isolated from non-pregnant cows and heifer uterus.Myometrial strips were isolated from cows and heifers that were at follicular phase and suspendedin a 5 mL jacketed organ bath filled with Krebs solution at 38 °C (pH 7.4) continuously bubbled with95% oxygen and 5% carbon dioxide. After manifestation of the spontaneous contractions duringequilibration period of 90 minutes, isometric contractions were recorded by using isometric forcedisplacement transducer and initial 10 minute was considered as control period. Then, the testsubstances ceftiofur (500 µM, cumulative 1000 µM and 2000 µM, n=16) were added to the tissuebath and evaluated by 10-min intervals. The effects of ceftiofur on amplitude (mg), frequency andarea under the contractile curve (AUC) of spontaneous contractions were evaluated by 10 minintervals. Data were statistically analyzed by using the Wilcoxon Signed Ranks Test and Pearson sCorrelation Test.It was determined that all tested doses of ceftiofur reduced the frequency of contractions (P0.05). After 500 µM and cumulative 1000 µM ceftiofur, theincrease in the AUC found to be statistically insignificant (P>0.05). But after addition of cumulative2000 µM ceftiofur the increase in the AUC found to be statistically significant (P

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1. Bajcsy AC, van der Weijden GC, Szenci O, Taverne MAM. A review on measurements of uterine contractility, with emphasis on spontaneous and drug-induced changes during the early postpartum period in cows. http://dspace.library.uu.nl/bitstream/handle/1874/7357/full. pdf?sequence=1/05.12.2013.
2. Cooper MD, Foote RH. Effect of oxytocin, prostaglandin F 2{alpha} and reproductive tract manipulations on uterine contractility in holstein cows on days 0 and 7 of the estrous cycle. J Anim Sci 1986; 63: 151-161.
3. Hertelendy F, Zakár T. Prostaglandins and the myometrium and cervix. Prostaglandins Leukot Essent Fatty Acids 2004; 70: 207-222.
4. Hirsbrunner G, Küpfer U, Burkhardt H, Steiner A. Effect of different prostaglandins on intrauterine pressure and uterine motility during diestrus in experimental cows. Theriogenology 1998; 50: 445-455.
5. Mitchell BF, Schmid B. Oxytocin and its receptor in the process of parturition. J Soc Gynecol Investig 2001; 8: 122- 133.
6. Baguma-Nibasheka M, Nathanielsz PW. In vivo administration of nimesulide, a selective PGHS2 inhibitor, increases in vitro myometrial sensitivity to prostaglandins while lowering sensitivity to oxytocin. J Soc Gynecol Invest 1998; 5: 296-299.
7. Bekyürek T. Koyunlarda Kuzulaman ı n Kontrolü Üzerinde Çal ı ş malar. Doktora Tezi, Ankara: Ankara Üniversitesi, Sa ğ l ı k Bilimleri Enstitüsü, 1989.
8. Scott JE, Grigsby PL, Hirst JJ, Jenkin G. Inhibition of prostaglandin synthesis and its effect on uterine activity during established premature labor in sheep. J Soc Gynecol Investig 2001; 8: 266-276.
9. Tsatsaris V, Cabrol D, Carbonne B. Pharmacokinetics of tocolytic agents. Clin Pharmacokinet 2004; 43: 833-844.
10. Risco CA, Hernandez J. Comparison of ceftiofur hydrochloride and estradiol cypionate for metritis prevention and reproductive performance in dairy cows affected with retained fetal membranes. Theriogenology 2003; 60: 47-58.
11. Lohuis JA, Van Leeuwen W, Verheijden JH, Brand A, Van Miert AS. Flunixin meglumine and flurbiprofen in cows with experimental Escherichia coli mastitis. Vet Rec 1989; 124: 305-308.
12. Morin DE, Shanks RD, McCoy GC. Comparison of antibiotic administration in conjunction with supportive measures versus supportive measures alone for treatment of dairy cows with clinical mastitis. J Am Vet Med Assoc 1998; 213: 676-684.
13. Öcal H, Yı ld ı z H, Ayd ı n M, Akar Y. İneklerde subklinik mastitisin danofloxacin ile tedavisi. FÜ Sa ğ Bil Derg 1999; 13: 399-404.
14. Friton GM, Cajal C, Ramirez Romero R, Kleemann R. Clinical efficacy of meloxicam (Metacam) and flunixin (Finadyne) as adjuncts to antibacterial treatment of respiratory disease in fattening cattle. Berl Munch Tierarztl Wochenschr 2004; 117: 304-309.
15. Jacobson GA, Martinod S, Cunningham CP. Determination of ceftiofur in bovine plasma by HPLC-DAD. J Pharm Biomed Anal 2006; 40: 1249-1252.
16. Amiridis GS, Leontides L, Tassos E, Kostoulas P, Fthenakis GC. Flunixin meglumine accelerates uterine involution and shortens the calving-to-first-oestrus interval in cows with puerperal metritis. J Vet Pharmacol Ther 2001; 24: 365-367.
17. Azawi OI. Postpartum uterine infection in cattle. Anim Reprod Sci 2008; 105: 187-208.
18. LeBlanc SJ. Postpartum uterine disease and dairy herd reproductive performance: A review. Vet J 2008; 176: 102- 114.
19. Palmer C. Postpartum metritis in cattle: A review of the condition and the treatment. Large Animal Veterinary Rounds 2003; (3)8: 1-6.
20. Beconi-Barker MG, Davison KL, Hornish RE, et al. [14C] Ceftiofur sodium absorption, distribution, metabolism, and excretion in sheep following ı ntramuscular Injections. J Agric Food Chem 1995; 43: 1589-1597.
21. Jaglan PS, Kubicek MF, Arnold TS, et al. Metabolism of ceftiofur. nature of urinary and plasma metabolites in rats and cattle. J Agric Food Chem 1989; 37: 1112-1118.
22. Okker H, Schmitt EJ, Vos PL, et al. Pharmacokinetics of ceftiofur in plasma and uterine secretions and tissues after subcutaneous postpartum administration in lactating dairy cows. J Vet Pharmacol Ther 2002; 25: 33-38.
23. Kasimanickam R, Duffield TF, Foster RA, et al. Endometrial cytology and ultrasonography for the detection of subclinical endometritis in postpartum dairy cows. Theriogenology 2004; 62: 9-23.
24. Roncada P, Ermini L, Schleuning A, Stracciari GL, Strocchia A. Pharmacokinetics and residual behaviour in milk of oxytetracycline in cows following administration of uterine pessaries. J Vet Pharmacol Ther 2000; 23: 281- 285.
25. Öcal H. İneklerde İntrauterin İlaç Uygulamas ı n ı n Ovaryum Faaliyetleri ve Uterus Üzerine Etkisi. Doktora Tezi, Elaz ı ğ : F ı rat Üniversitesi, Sa ğ l ı k Bilimleri Enstitüsü, 1991.
26. Drillich M, Arlt S, Kersting S, et al. Ceftiofur derivatives in serum, uterine tissues, cotyledons, and lochia after fetal membrane retention. J Dairy Sci 2006; 89: 3431-3438.
27. Königsson K, Gustafsson H, Gunnarsson A, Kindahl H. Clinical and bacteriological aspects on the use of oxytetracycline and flunixin in primiparous cows with induced retained placenta and post-partal endometritis. Reprod Dom Anim 2001; 36: 247-256.
28. Hanzen CH, Pietrose M, Scenczi O, Drost M. Relative accuracy of the identification of ovarian structures in the cow by ultrasonography and palpation per rectum. Vet J 2000; 159: 161-170.
29. Ireland JJ, Murphee RL, Coulson PB. Accuracy of predicting stages of bovine estrous cycle by gross appearance of the corpus luteum. J Dairy Sci 1980; 63: 155-160.
30. Ocal H, Yuksel M, Ayar A. Effects of gentamicin sulfate on the contractility of myometrium isolated from non-pregnant cows. Anim Reprod Sci 2004; 84: 269-277.
31. Melendez P, McHale J, Bartolome J, Archbald LF, Donovan GA. Uterine involution and fertility of holstein cows subsequent to early postpartum PGF 2 alpha treatment for acute puerperal metritis. J Dairy Sci 2004; 87: 3238-3246.
32. Servi K, Kara H. İn vitro kedi myometriumu üzerine gentamisinin farkl ı dozları n ı n etkisi. FÜ Sa ğ Bil Derg 2001; 15: 381-386.
33. Servi K, Kara H. Oksitosinle kontraktile edilen in vitro s ı ğ ı r myometriumu üzerine gentamisinin farkl ı dozları n ı n etkileri. FÜ Sa ğ Bil Derg 2001;15: 189-194.
34. Servi K, Kara H. Spontan ve oksitosinle indüklenen rat uterus kontraksiyonları üzerine farkl ı dozlarda gentamisin ve eritromisin etkilerinin in vitro ara ş tı rı lmas ı . FÜ Sa ğ Bil Derg 2001; 15: 199-206.
35. Celik H, Ayar A. Clarithromycin inhibits myometrial contractions in isolated human myometrium independent of stimulus. Physiol Res 2002; 51: 239-245.
36. Ocal H, Yuksel M, Ayar A. Gentamycin inhibition of KCl- induced contractions of myometrium isolated from non- pregnant and pregnant cows. Vet Med-Czech 2004; 49: 401-405.
37. Servi K, Kara H. Oksitoksinle uyarı lan in vitro s ı ğ ı r myometriumu üzerine eritromisinin farkl ı dozları n ı n etkileri. FÜ Sa ğ Bil Derg 2001;15: 179-184.
38. Servi K, Kara H. Oksitosinle kontraktile edilen in vitro kedi myometriumuna eritromisinin etkisinin ara ş tı rı lmas ı . FÜ Sa ğ Bil Derg 2001; 15: 375-380.
39. Celik H, Ayar A, Baltaci A, Tug N. Erythromycin inhibits prostaglandin F 2 alpha-induced contractions of myometrium isolated from non-pregnant rats. BJOG 2002; 109: 1036-1040.
40. Galvão KN, Greco LF, Vilela JM, Sá Filho MF, Santos JE. Effect of intrauterine infusion of ceftiofur on uterine health and fertility in dairy cows. J Dairy Sci 2009; 92: 1532-1542.
41. Chastant-Maillard S, Aguer D. Pharmacologie de l'utérus infecté: facteur de choix d'une thérapeutique. In: Navetat H, Schelcher F. (Editors). Le Nouveau Peripartum. Toulouse, France: Buiatri, 1998; 167-187.