Songül ÇERİBAŞI, Dilek ATEŞŞAHİN, Ahmet ATEŞŞAHİN

2,3,7,8-Tetraklorodibenzo-p-dioksin ile Rat Beyin ve Kalp Dokusunda Oluşturulan Toksisite Üzerine Melatoninin Etkileri

Bu çalışmada ratlarda subakut 2,3,7,8-tetraklorodibenzo-p-dioksin (TCDD)'in beyin ve kalp dokusu üzerine toksik etkileri ve bu etkilere karşı melatoninin koruyuculuğu araştırıldı.Çalışmada 230-250 g ağırlığında 28 adet Sprague-Dawley ırkı erkek rat kullanıldı ve her grupta 7 adet hayvan olacak şekilde ayrıldı. Çalışma süresi 30 gün olarak belirlendi. Hayvanlar kontrol grubu, melatonin grubu (5 mg/kg melatonin periton içi yolla verildi), TCDD grubu (500 ng/kg TCDD gavajla verildi) ve TCDD+melatonin grubu (eş zamanlı 5 mg/kg melatonin periton içi yolla ve 500 ng/kg TCDD gavajla verildi) olarak dört gruba ayrıldı. Son ilaç uygulamasından 24 saat sonra ratlar dekapite edilerek biyokimyasal analizler için beyin ve kalp dokuları alındı. Dokuların malondialdehit (MDA) ve redükte glutatyon (GSH) düzeyleri ile katalaz (CAT) aktivitesi spektrofotometrik olarak ölçüldü. Kalp dokusu ayrıca histopatolojik olarak incelendi.Beyin ve kalp dokusu MDA düzeylerinin TCDD gruplarında arttığı (P0.05). Beyin ve kalp dokusu CAT aktivitelerinde ise tüm gruplarda değişiklik görülmedi (P>0.05). Ayrıca TCDD'nin kalp dokusunda histopatolojik değişimlere neden olduğu ve TCDD+melatonin grubunda melatoninin bu değişimleri azalttığı gözlenmiştir. Sonuç olarak bu çalışma TCDD'nin serbest radikal oluşumunu artırarak beyin ve kalp dokusunda toksisiteye neden olduğunu, buna karşılık melatoninin oluşan oksidatif ve histopatolojik hasarlarda iyileşmeler sağladığını açıkçagöstermektedir.

The Effects of Melatonin on 2,3,7,8-Tetrachlorodibenzo-p-Dioxin Induced Toxicity in Rat Brain and Heart

In this study,the toxic effects of subacute 2,3,7,8-tetrachlorodibenzo-p-dioxin on brain and heart tissues and the protection of melatonin against these effects in rats were investigated.A total of 28 male Sprague-Dawley rats weighing between 230-250 g were used in the present study and divided into groups as seven rats in each group. The experimental period was 30 days. Animals were divided into four groups, as control group, melatonin group (5 mg/kg dose of melatonin was given), TCDD group (500 ng/kg TCDD was given), TCDD+melatonin group (500 ng/kg TCDD and 5 mg/kg melatonin were given simultaneously). After 24 hours of the last drug application,rats were decapitated and their brain and heart tissues were removed for biochemical analysis. The tissuemalondialdehyde (MDA), reduced glutathione (GSH) levels and catalase (CAT)activitiesweremeasuredspectrophotometrically.Hearttissue was alsoexamined histopathologically.MDA levels of brain and heart tissues were determined to increasein TCDD groups (P0.05). No changein CAT activities was observed in eitherbrain and heart tissues in all groups (P>0.05). In addition TCDD was observed to causehistopathological changes in hearttissue and melatonin to reducethese changes in TCDD+melatonin group.Consequently,this study clearly indicated that TCDD induced toxicity by increasing the formation of free radicalsin brain and hearth tissues, while melatonin might provide improvements on oxidative and histopathological damage.

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