Protective Effect of Ginkgo Biloba Extract 761 on Di-(2-ethylhexyl) Phthalate-Induced Testicular Toxicity in Prepubertal Rats

Bu çalışmada, ratlara farklı sürelerde aynı dozda di-(2-etilhekzil) fitalat (DEHP) verilerek oluşturulan testiküler hasar üzerine ginkgo biloba ekstrakt 761 (EGb 761)'in koruyucu etkisi incelendi. Yetmiş iki adet 28 günlük Wistar Albino cinsi rat; kontrol, DEHP, DEHP + EGb 761 ve EGb 761 gruplarına ayrıldı. Çalışmanın 7., 14., ve 28. günlerinde hayvanlar dekapite edildi. DEHP uygulamasının kontrol grubu ile karşılaştırıldığında; malondialdehit (MDA), glutatyon peroksidaz (GSH-Px) ve katalaz (CAT) seviyelerinde, testiküler dokuda dejenerasyon, nekroz, atrofi, disorganizasyon, Leydig hücre hiperplazisi ve bazal membran kalınlaşması ile birlikte apoptotik indekslerde belirgin artışlara sebep olmasına karşın, testislerin ağırlıklarında, seminifer tubüllerin çaplarında, germinal hücre tabakası kalınlıklarında, serum testosteron ve glutatyon (GSH) seviyelerinde belirgin azalışlara neden olduğu belirlendi. DEHP uygulanan ratlara EGb 761 verilmesi ile bozulmuş oksidan/antioksidan dengesinde, azalmış testosteron konsantrasyonunda, indüklenmiş testiküler apoptoziste belirgin gelişme ve testiküler dokunun histopatolojik görünümünde kademeli olarak düzelme sağlandığı gözlendi. DEHP' ın uygulanan süre ve dozda testis dokusu için son derece toksik olduğu ve ciddi yapısal hasara sebep olduğu; antioksidan etkiye sahip olan EGb 761'in ise büyük ölçüde bu hasarın önüne geçtiği tespit edildi

Prepubertal Ratlarda Di-(2-Etilhekzil) Fitalat'ın Oluşturduğu Testiküler Toksisite Üzerine Ginkgo Biloba Ekstrakt 761'in Koruyucu Etkisi

In this study, the protective effect of ginkgo biloba extract 761 (EGb 761)'s on testicular damage caused analyzed by giving the same dose di-(2-ethylhexyl) phthalate (DEHP) to rats at different times was investigated. 72 Wistar Albino rats at 28 days of age were divided into groups of control, DEHP, DEHP+EGb 761 and EGb 761. On the 7th,14th and 28th days of the study, the animals were decapitated. DEHP administration was determined to cause significant decreases in testes weights, diameter of seminiferous tubules, germinal cell layer thickness, serum testosterone levels, glutathione (GSH) levels wherease it caused significant increases in malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and catalase (CAT) levels, apoptotic indices along with degeneration, necrosis, atrophy, disorganization, Leydig cell hyperplasia and thickening of the basal membrane in testicular tissue when compared with the control group. EGb 761 administration provided significant improvements in disturbed oxidant/antioxidant balance, decreased testosterone concentration, induced testicular apoptosis and gradually improvements in the histopathological view of the testicular tissue in DEHP-treated rats. It was determined that DEHP is highly toxic to the testis tissue for the application duration and dose and cause to severe structural damage and EGb 761 which has antioxidant effect prevents this damage

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