Metin GÜRÇAY, Mustafa İSSİ, Yusuf GÜL, Taşkın GÖK

Elazığ yöresindeki koyunlarda saptanan pestivirus enfeksiyonu

Pestivirus infection determined in sheep in Elazığ region

This study aims to report pestivirus infection determined in the sheep of Akkaraman race in Elazığregion and to emphasize the economical significance of the infection. The study material included 8 different flocks having abortion incidents and consisting of total 4272 Akkaraman breed sheep in Elazığ province between January and February 2012. For analyzing bovine viral diarrhea virus (BVDV) antibodies, the blood and tissue samples obtained from 20 fetus and a total of 219 aborted ewes which corresponded to almost %5 of the sheep from the aborted flocks were sent toElazığ Veterinary Control Institution. The presence of BVDV antibodies in sheep having abortion were examined using BVDV total antibodies ELISA, and the presence of BVDV antibodies in aborted fetuses were examined using BVDV antigen ELISA kits. BVDV antibodies was detected in 65.75% (144/219) of the blood samples and BVDV antigen was detected in 55% (11/20) of the fetus tissue samples. 4.9% (210/4272) of the sheep included in the study had abortion, 4.5% (204/4272) were determined to become barren, 5.0% (215/4272) to have growth retardation, 0.4% (19/4272) to have anomaly and 3.4% (137/4272) to die. In conclusion, determining the presence of pestivirus infection in aborted sheep, this study suggests to consider the effect of this infection in abortion incidents in sheep which cause significant economic damages and to take protective measures.

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1. Radostits OM, Gay CC, Hinchcliff KW, Constable PD. Veterinary Medicine. Tenth edition, Edinburgh, London, New York, Oxford, Philadelphia, St Louis, Sydney, Toronto: Saunders Elsevier, 2008.
2. Scott PR. Sheep Medicine. London: Manson Publishing Ltd, 2007.
3. O’Neill RG, O’Connor M, O’Reilly PJ. A survey of antibodies to pestivirus in sheep in the Republic of Ireland. Irish Vet J 2004; 57: 525-530.
4. Becher P, Avalos Ramirez R, Orlich M, et al. Genetic and antigenic characterization of novel pestivirus genotypes: implications for classification. Virology 2003; 311: 96-104.
5. Thiel HJ, Collett MS, Gould EA, et al. Family Flaviviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (Editors). Virus Taxonomy. Eighth report of the International Committee on Taxonomy of Viruses. London: Elsevier, 2005: 978-987.
6. Oğuzoğlu Ç. Sınır hastalığı (Border disease). Ankara Üniv Vet Fak Derg 2008; 55: 69-74.
7. Plant JW, Littlejohns IR, Gardiner AC, Vansis JT, Muck RA. Immunological relationship between border disease, mucosal disease and swine fever. Vet Rec 1973; 92: 455.
8. Van Oirschot JT. Congenital infections with nonarbotogaviruses. Vet Microbiol 1983; 8: 321-361.
9. Carlsson U, Belak K. Border disease virus transmitted to sheep and cattle by a persistently Infected ewe: Epidemiology and control. Acta Vet Scand 1994; 35: 79-88.
10. Krametter-Froetscher R, Duenser M, Preyler B, et al. Pestivirus infection in sheep and goats in West Austria. Vet J 2010; 186: 342-346.
11. Schaller P, Vogt HR, Strasser M, et al. Seroprevalence of maedi-visna and border disease in Switzerland. Schweiz Arch Tierheilkd 2000; 142: 145-153.
12. Lamontagne L, Roy R. Presence of antiboties to bovine viral diarrhoea-mucosal disease birus (Border disease) in sheep and goat flocks in Quebec. Can J Comp Med 1984; 48: 225-227.
13. Loken T, Krosgrud J, Larsen IL. Pestivirus infections in Norway. Serological investigation in cattle, sheep and pisg. Acta Vet Scand 1991; 32: 27-34.
14. Soydal Ataseven V, Ataseven L, Tan T, Babur C, Oguzoglu TC. Seropositivity of agents causing abortion in local goat breeds in Eastern and Southeastern Anatolia, Turkey. Rev Med Vet 2006; 157: 955-961.
15. Gazyagcı S, Azkur AK, Çağlayan O. Comparison of hematological and biochemical parameters in sheep naturally and pesistently infected with a border disease virus. Trop Anim Health Prod 2011; 43: 553-556.
16. Gür S. A investigation of border disease virus in sheep in Western Turkey. Trop Anim Health Prod 2009; 41: 1409- 1412.
17. İssi M, Gülaçtı İ, Kızıl Ö, et al. Kliniğimizde gözlemlenen reverze transkriptaz–polimeraz zincir reaksiyonu (RT-PCR) ile doğrulanan mukoza hastalığı olguları. Fırat Üniv Sağlık Bil Derg 2006; 20: 253-258.
18. Burgu İ, Akça Y, Alkan F, et al. Koyunlarda doğum öncesi ve sonrası dönemlerde bovine viral diarrhea (BVD) enfeksiyonunun serolojik, virolojik ve patogenez yönünden araştırılması. Turk J Vet Anim Sci 2001; 25: 551-557.
19. Avcı O. Konya ve Çevresinde Abort Problenli Koyun Sürülerinde Border Disease Virüs Enfeksiyonun Araştırılması. Doktora tezi, Konya: Selçuk Üniversitesi, Sağlık Bilimleri Enstitüsü, 2010.
20. Azkur AK, Gazyağcı S, Aslan ME, Unal N. Molecular and serological characterization of pestivirus infection among sheep in Kırıkkale, Turkey. Kafkas Univ Vet Fak Derg 2011; 17 (Suppl A): S83-S92.
21. Oguzoglu CT, Tan MT, Toplu N, et al. Border disease virus (BDV) infections of small ruminants in Turkey: A new BDV subgroup? Vet Microbiol 2009; 135: 374-379.
22. Ozan E, Turan HM, Albayrak H, Cavunt A. Serological determination of pestivirus, bluetongue virus and peste des petits ruminants virus in small ruminants in Samsun province of Turkey. Atatürk Üniv Vet Bil Derg 2012; 1: 27- 33.
23. Hasırcıoğlu S, Kale M, Acar A. Investigation of pestiviruses infections in aborted sheep and goats in Burdur region. Kafkas Univ Vet Fak Derg 2009; 15: 163-167, 2009.
24. Okur-Gumusova S, Yazıcı Z, Albayrak, H. Pestivirus seroprevalence in sheep populations from inland and coastal zones of Turkey. Rev Med Vet 2006; 157: 595-598.
25. Burgu İ, Öztürk F, Akça Y, et al. Investigations on the occurrence and impact of bovine viral diarrhea (BVD) virus infections in sheep in Turkey. Dtsch Tierarztl Wschr 1987; 94: 292-294.
26. Albayrak H, Ozan E. The investigation of pestivirus and rift valley fever virus infections in aborted ruminant foetuses in the Blacksea region in Turkey. Kafkas Univ Vet Fak Derg 2012; 18: 457-461.
27. Garcia-Perez AL, Miguijon E, Barandika JF, et al. Detection of border disease virus in fetuses, stillbirths, and newborn lambs from natural and experimental infections. J Vet Diagn Invest 2009; 21: 331-337.
28. Nettleton PF, Gilray JA, Russo P, Dlissi E. Border disease of sheep and goats. Vet Res 1998; 29: 327-340.
29. Anderson CA, Sawyer M, Higgins RD, East N, Osburn BL. Experimentally induced ovine border disease: Extensive hypomyelination with minimal viral antigen in neonatal spinal cord. Am J Vet Res 1987; 48: 499-503.
30. Gardiner AC. The distribution and significance of border disease viral antigen in infected lambs and foetuses. J Comp Path 1980; 90: 513-518.
31. Hewicker–Trautwein M. Pestivirus – induzierte Alterationen im Zentralnervesystem von Wiederkaeuern. Tieraerztl Prax 1994; 22: 516-523.
32. Nettleton PF. Pestivius infections in ruminants other than cattle. Rev Sci Tech Off Int Epiz 1990; 9: 131-150.
33. Waldvogel AS, Ehrensperger F, Straub OC, Pospisil A. An immunohistochemical study of the distribution of border disease virus in persistently infected sheep. J Comp Pathol 1995; 113: 191-200.
34. Sharp MW, Rawson BC. The cost of Border disease infection in a commericial flock. Vet Rec 1986; 119: 128- 130