Mehmet KÖSE, Şükrü DURSUN, Mehmet ÇOLAK, Bülent BÜLBÜL, Mesut KIRBAŞ

Düvelerde dondurulmuş embriyoların transferinde embriyonun uterusta bırakıldığı Kornu uteri ve derinliğinin gebelik oranı üzerine etkisi

Bu çal ı ş ma, düvelerde dondurulmu ş embriyoları n transferinde embriyonun korpus luteumunbulundu ğ u ovaryuma göre b ı rakı ld ı ğ ı kornu uteri (ipsilateral/kolleteral) ve derinli ğ inin(derin/yüzeysel) gebelik oran ı üzerine etkisini belirlemek amacı yla yap ı ld ı . Derin transfer,embriyonun kornu uterinin, bifurkasyo bölgesinden sonraki üçüncü 1/3lük kı sm ı na b ı rakı lmas ıolarak tan ı mlan ı rken, yüzeysel transfer ise embriyonun kornu uterinin bifurkasyo bölgesindensonraki ilk 1/3lük k ı sm ı na b ı rakı lmas ı olarak tan ı mland ı . Bu tan ı mlamalara ve korpus luteumunlokalizasyonuna göre ta ş ı yı c ı lar rasgele dört gruba ayrı ld ı . Etilen glikolle direk transfer metodunagöre dondurulmu ş birinci kalite embriyolar ta ş ı y ı cı lara östrüs (0. gün) sonras ı 7. günde; Grup Idederin-ipsilateral (n=12), Grup IIde yüzeysel-ipsilateral (n=13), Grup IIIde derin-kolleteral (n=13),Grup IVte ise yüzeysel-kolleteral (n=12) olarak transfer edildi. Ta ş ı yı cı ları n gebelik muayeneleri 28.günde trans-rektal ultrasonografi ile yap ı ld ı . Gebelik oranları gruplarda (Grup I, Grup II, Grup III veGrup IV) s ı ras ı yla %41.7 (5/12), %61.5 (8/13), %30.8 (4/13) ve %25 (3/12) tespit edildi. Grupları ngebelik oranları aras ı ndaki farkl ı l ı k istatistiki olarak önemli bulunmadı (P>0.05). Yap ı lan transferlersadece derin ve yüzeysel olarak grupland ı rı ld ı ğ ı nda gebelik oranları s ı ras ı yla; %36 (9/25) ve %40(11/25) oldu, fakat gebelik oranları aras ı ndaki farkl ı l ı k önemli olarak tespit edilmedi (P>0.05).Transferler sadece ipsilateral ve kolleteral olarak grupland ı rı ld ı ğ ı nda ise gebelik oranları s ı ras ı yla%52 (13/25) ve %28 (7/25) oldu. İstatistiksel olarak ipsilateral transfer grubunda elde edilen gebelikoran ı , kolleteral transferlerden yüksek olma e ğ iliminde oldu ğ u görüldü (P=0.07). Sonuç olarak,etilen glikolle dondurulmu ş birinci kalite embriyoları n korpus luteumla ayn ı taraftaki kornu uteriyeb ı rak ı lmas ı gerekti ğ i kan ı s ı na varı ld ı .

Effect of the depth and site of embryo deposition on pregnancy rate after embryo transfer in heifers

This study was conducted to determine the effects of both the uterine horn of the cryopreservedembryo placement according to corpus luteum, ipsilateral or colleteral, and the depth of embryoplacement in the uterine horn on pregnancy rates in heifers. Deep transfer was defined as theinjection of the embryo in third one-third of the cornu uterine, while shallow transfer was defined asthe injection of embryo in first one-third stage of the cornu uterine. According to this definition andlocation of the corpus luteum, recipients were divided into four different groups. On day 7 afteroestrus, embryos, grade1 embryos frozen with ethylene glycol by direct transfer method, weretransferred deep-ipsilateral in Group I (n=12), shallow-ipsilateral in Group II (n=13), deep-collateralin Group III (n=13) or shallow-collateral in Group IV (n=12). Pregnancies were examined 28 daysafter transfer by ultrasonography. Pregnancy rates in Group I, II, III and IV were 41.7% (5/12),61.5% (8/13), 30.8% (4/13), and 25% (3/12), respectively. The differences among groups werenon-significant (P>0.05). When transfers are analyzed as deep and shallow groups, the pregnancyrates were 36% (9/25) in deep group, 40% (11/25) in shallow group, but the difference was notsignificant. If transfers are evaluated as ipsilateral and colleteral, pregnancy rates were 52%(13/25) in ipsilateral group and 28% (6/25) in collateral group. Pregnancy rate in ipsilateral transferstended to be higher than in collateral transfers (P=0.07). It is concluded that the embryo should beplaced in the uterine horn ipsilateral to the corpus luteum in direct transfer.

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