Ebubekir CEYLAN, Semih YAŞAR, Fatmagül YUR

Doksorubisin Uygulanan Ratlarda COQ10 ve Vitamin E’nin Kanda Lipit Peroksidasyonu, Bazı Antioksidan ve Biyokimyasal Parametrelere Etkisi

Bu çalışmada doksorubisin uygulanan ratlarda, Koenzim $Q_10$ ($CoQ_10$) ve vitamin E’in lipitperoksidasyonu, antioksidan profil ve bazı biyokimyasal parametreler üzerine etkisi araştırıldı.Çalışmada altı aylık 40 adet Wistar-Albino ırkı erkek rat kullanıldı. Hayvanlar dört gruba ayrıldı.Birinci grup ratlara doksorubisin 2.5 mg/kg/serum fizyolojik i.p. olarak haftada bir kez 6 haftasüreyle uygulandı. İkinci grup ratlara doksorubisine ilave olarak $CoQ_10$ 4 mg/kg/canlı ağırlıkoranında i.p. olarak hergün uygulandı. Üçüncü grup ratlara sadece $CoQ_10$ 4 mg/kg/canlı ağırlıkoranında yine günlük olarak verildi. Dördüncü grup ratlara doksorubisine ilave olarak vitamin E 10mg/kg/subkutan/haftada iki kez 6 hafta süreyle uygulandı. Çalışma süresi altı hafta olarakplanlandı. Çalışmaya başlamadan önce ratlardan alınan kanlar kontrol grubu oluşturmak amacıylakullanıldı. Altıncı hafta sonunda tüm ratlardan kan örnekleri alındı. Analizler sonucunda tümgruplarda malondialdehit ve seruloplazmin düzeylerinde önemli artışlar gözlendi. Tüm gruplardaglutatyon düzeylerinde kontrol grubuna göre düşüşler gözlendi. Retinol ve α-tocopheroldüzeylerinde farklılık gözlenmedi. Albumin düzeylerinde önemli azalmalar ve globülin düzeylerindeönemli artışlar belirlendi. Total protein düzeylerinde önemli artışlar, alanin aminotransferazdüzeylerinde DXR grubunda önemli bir artış diğer guruplarda düşüşler gözlendi. Glukozdüzeylerinde ise belirgin düşüşler görüldü. Çalışmada DXR’nin oksidatif etkisi belirgin bir şekildegörüldü ancak verilen $CoQ_10$ ve vitamin E’nin antioksidan etkileri istenilen düzeyde belirgin olarakgözlenemedi. Çalışma verilerine göre $CoQ_10$ ve vitamin E gibi antioksidan maddelerin, çeşitliilaçların neden olduğu stres ve hasarı azaltmak için kullanılabileceği ancak verilen ilaçların kullanımsüresinin antioksidan maddelerin başarısını azaltabileceği sonucuna varıldı.

Effects of $CoQ_10$ and Vitamin E on Blood Lipid Peroxidation,Some Antioxidant and Biochemical Parameters inDoxorubicin Administered Rats

This study was aimed to investigate the effects of vitamin E and Coenzyme $Q_10$ ($CoQ_10$) on lipidperoxidation, antioxidant profile and some biochemical parameters in the doxorubicin administered rats. 40 Wistar-Albino breed male, 6 months old rats were used. The animals were divided into fourequal groups. The first group received doxorubicin (hydroxy daunorubicin) (2.5 mg/kg/week/i.p.).The second group was given $CoQ_10$ (4 mg/kg/daily/i.p.) in addition to doxorubicin. The third groupreceived only $CoQ_10$ (4 mg/kg/daily/i.p.). The fourth group was dministered with vitamin E (10 mg(15 IU) /kg/subcutaneous/two times a week), in addition to doxorubicin. The study period was sixweeks. The blood samples taken from the rats before the beginning of the experiment was used asthe control group. At the end of the 6th- week, the blood samples were taken from all rats. As aresult of the analysis, significant increases were observed in malondialdehyde and ceruloplasminlevels in all groups. Glutathione levels were decreased in all groups compared to the control group.No difference was observed in retinol and α-tocopherol levels. Significant decreases in albuminlevels and significant increases in globulin levels were determined. Significant increases in totalprotein levels, significant increases in alanine aminotranferase levels in the DXR group, anddecreases in other groups were observed. Significant decreases were observed in glucose levels.In the study, the oxidative effect of DXR was clearly seen, but the antioxidant effects of $CoQ_10$ andvitamin E were not observed at the desired level. According to the data of our study, it wasconcluded that antioxidant substances such as $CoQ_10$ and vitamin E can be used to reduce stressand damage caused by various drugs, but the duration of use of the given drugs may reduce thesuccess of antioxidant substances.

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