Emin KARAKURT, Serpil DAĞ, Ayfer YILDIZ, Hilmi NUHOĞLU, Enver BEYTUT, Özhan KARATAŞ, Ali Sefa MENDİL

Beyaz Kas Hastalıklı Kuzularda 4-Hidroksi-2-Nonenal, Ditirozin and 8-Hidroksi-2- Deoksiguanozin Ekspresyonlarının Değerlendirilmesi

Bu çalışmada, beyaz kas hastalıklı kuzularda 4-hidroksi-2-nonenal (4-HNE), ditirozin (DT) ve 8- hidroksi-2-deoksiguanozin (8-OHdG) gibi oksidatif stres belirteçleri ile Reaktif Oksijen Türleri(ROT) kaynaklı lipid peroksidasyonu, protein ve DNA hasarının araştırılması amaçlanmıştır. Buçalışmanın materyalini 2012-2019 yılları arasında rutin histopatolojik tanı için Patoloji AnabilimDalı’na getirilen beyaz kas hastalıklı 20 koyundan alınan doku örnekleri ve 6 sağlıklı kontrol grubuoluşturdu. 4-HNE, DT ve 8-OHdG ekspresyonları immünohistokimyasal ve immünofloresanyöntemlerle değerlendirildi. Makroskopik incelemelerde epikardiyal ve özellikle endokardiyalyüzeylerde, sağ ve sol ventrikül duvarlarında tebeşirimsi beyaz büyük nekrotik alanlar gözlemlendi.Mikroskobik incelemelerde kalpteki dejenere olmuş kas liflerinin homojen pembe renkte olduğunu,sitoplazmik sitrifikasyonunu kaybettiğini, şiştiği ve çekirdeklerinin piknotik olduğu gözlemlendi.Nekrotik ve dejenere alanlarda kalsifikasyonlar tespit edildi. Tüm beyaz kas hastalığı vakaları kalpdokularında 4-HNE, DT ve 8-OHdG ekspresyonu yönünden immün pozitifti. İmmünohistokimyasalve immünofloresan boyama sonuçlarında 4-HNE, DT ve 8-OHdG'ye karşı immünreaktiviteaçısından gruplar arasında anlamlı farklılık (P

Evaluation of 4-Hydroxy-2-Nonenal, Dityrosine and 8- Hydroxy-2-Deoxyguanosine Expressions in Lambs withWhite Muscle Disease

The aim of this study was to investigate Reactive Oxygen Species (ROS)-induced lipidperoxidation, protein and DNA damage with oxidative stress markers such as 4-hydroxy-2- nonenal (4-HNE), dityrosine (DT) and 8-hydroxy-2-deoxyguanosine (8-OHdG) in lambs withWhite Muscle Disease (WMD). The material of this study consisted of tissue samples taken from20 sheep with WMD and 6 healthy control groups brought to Pathology Department for routinehistopathological diagnosis between 2012 and 2019. In macroscopic examinations, chalky-whitelarge necrotic areas on the epicardial and especially on the endocardial surfaces of the right andleft ventricular walls were observed. In microscopic examinations, it was observed that thedegenerated muscle fibers in the heart were homogeneously pink in color, lost their cytoplasmiccitration, swelled and their nuclei were pyknotic. Calcifications in necrotic and degenerated areaswere detected. All white muscle disease cases were immune positive for 4-HNE, DT and 8-OHdGexpressions in heart tissues. A significant difference (P

PDF

___

1. Yildirim S, Ozkan C, Huyut Z, Çınar A. Detection of Se, Vit E, Vit A, MDA, 8-OHdG, and CoQ10 levels and histopathological changes in heart tissue in sheep with white muscle disease. Biol Trace Elem Res 2019; 188: 419-423.
2. Mert H, Yildirim S, Yoruk IH, et al. Retinol, α-tocopherol and vitamin D3 in white muscle disease. Med Weter 2018; 74: 441-444.
3. Ataollahi F, Mohri M, Seifi HA, et al. Evaluation of copper concentration in subclinical cases of white muscle disease and its relationship with cardiac troponin I. PLoS One 2013; 8: e56163.
4. Yavuz O. The pathological investigations on nutritional myopathy causing lamb deaths in neonatal period. Bahri Dağdaş Hayvancılık Araştırma Dergisi 2017; 6: 1-8.
5. Beytut E, Erişir M, Aksakal M. Beyaz kas hastalıklı kuzuların kalp, iskelet kası ve karaciğerlerinde redükte glutatyon ve malondialdehit düzeyleri ile katalaz enzim aktivitesi. Kafkas Üniv Vet Fak Derg 2001; 7: 1-5.
6. Loukopoulos P, Ioannou I, Kassinis N, et al. Congenital nutritional myodegeneration in a Cypriot mouflon (Ovis orientalis ophion) lamb. Revue Mèd Vèt 2012; 163: 402- 404.
7. Yildirim S, Irak K, Mert H, Dogan I, Mert N. Serum biochemical and histopathological examinations of some tissues of lambs with muscular dystrophy in Van. Sci Work Series C Veterinary Med 2016; 2: 52-55.
8. Başbuğan Y, Yüksek N, Altuğ N, Ceylan E, et al. The diagnostic significance of serum troponin levels lambs with white muscle disease. Turkish Journal of Veterinary Research 2018; 2: 5-11.
9. Değer Y, Mert H, Mert N, et al. Serum selenium, vitamin E, and sialic acids concentrations in lambs with white muscle disease. Biol Trace Elem Res 2008; 121: 39-43.
10. Or ME, Dodurka HT, Kayar A. Beyaz kas hastalıklı kuzularda eritrosit glutasyon peroksidaz (GSH-Px) aktivitesi ve bazı serum parametrelerinin diagnostik önemi. Turk J Vet Anim Sci 2003; 27: 1-6.
11. Kozat S, Gunduz H, Deger Y, et al. Studies on serum alpha-tocopherol, selenium levels and catalase activities in lambs with white muscle disease. Bull Vet Inst Pulawy 2007; 51: 281-284.
12. Kozat S, Altuğ N, Yüksek N, Özkan C. Evaluation of the levels of homocysteine, troponin I, and nitric oxide in lambs with subclinical white muscle disease. Kafkas Univ Vet Fak Derg 2011; 17: 441-444.
13. Yumusak N, Yigin A, Polat PF, Hitit M, Yilmaz R. Expression of ADAMTS-7 in myocardial dystrophy associated with white muscle disease in lambs. Pol J Vet Sci 2018; 21: 119-126.
14. Bildik A, Yur F, Çamaş H, Dede S, Sekin S. Beyaz kas hastalıklı kuzularda hemoglobin tipleri ile lipid peroksidasyonu ve glutatyon düzeylerinin araştırılması. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi 1996; 7: 95- 98.
15. Breitzig M, Bhimineni C, Lockey R, Kolliputi N. 4-Hydroxy2-nonenal: A critical target in oxidative stress? Am J Physiol Cell Physiol 2016; 311: C537-C543.
16. Smathers RL, Fritz KS, Galligan JJ, et al. Characterization of 4-HNE modified L-FABP reveals alterations in structural and functional dynamics. PLoS One 2012; 7: e38459.
17. Ayala A, Muñoz MF, Argüelles S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev 2014; 2014: 360438.
18. Lee WC, Wong HY, Chai YY, et al. Lipid peroxidation dysregulation in ischemic stroke: plasma 4-HNE as a potential biomarker? Biochem Biophys Res Commun 2012; 425: 842-847.
19. Pillon NJ, Croze ML, Vella RE, et al. The lipid peroxidation by-product 4-hydroxy-2-nonenal (4-HNE) induces insulin resistance in skeletal muscle through both carbonyl and oxidative stress. Endocrinology 2012; 153: 2099-2111.
20. Wu GR, Cheserek M, Shi YH, et al. Elevated plasma dityrosine in patients with hyperlipidemia compared to healthy individuals. Ann Nutr Metab 2015; 66: 44-50.
21. Li B, Ge Y, Xu Y, et al. Spatial learning and memory impairment in growing mice induced by major oxidized tyrosine product dityrosine. J Agric Food Chem 2019; 67: 9039-9049.
22. Mongirdienė A, Laukaitienė J, Skipskis V, Kašauskas A. The effect of oxidant hypochlorous acid on platelet aggregation and dityrosine concentration in chronic heart failure patients and healthy controls. Medicina (Kaunas) 2019; 55: 198.
23. Mayer F, Falk M, Huhn R, Behmenburg F, Ritz-Timme S. Dityrosine as a marker of acute myocardial infarction? Experiments with the isolated Langendorff heart. Int J Legal Med 2016; 130: 1053-1060.
24. Valavanidis A, Vlachogianni T, Fiotakis C. 8-hydroxy-2' - deoxyguanosine (8-OHdG): A critical biomarker of oxidative stress and carcinogenesis. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev 2009; 27: 120-139.
25. Kroese LJ, Scheffer PG. 8-hydroxy-2'-deoxyguanosine and cardiovascular disease: A systematic review. Curr Atheroscler Rep 2014; 16: 452.
26. Di Minno A, Turnu L, Porro B, et al. 8-Hydroxy-2- Deoxyguanosine levels and cardiovascular disease: A systematic review and meta-analysis of the literature. Antioxid Redox Signal 2016; 24: 548-555.
27. Alpar R. Spor, Sağlık ve Eğitimli Bilimlerinden Örnekle Uygulamalı İstatistik ve Geçerlik-Güvenlik SPSS’de Çözümleme Adımları ile Birlikte. 6. Baskı, Ankara: Detay Yayıncılık, 2020.
28. Tunca R, Erdoğan HM, Sözmen M, et al. Evaluation of cardiac troponin I and inducible nitric oxide synthase expressions in lambs with white muscle disease. Turk J Vet Anim Sci 2009; 33: 53-59.
29. Gunes V, Ozcan K, Citil M, Onmaz AC, Erdogan HM. Detection of myocardial degeneration with point-of-care cardiac troponin assays and histopathology in lambs with white muscle disease. Vet J 2010; 184: 376-378.
30. Hanedan B, Erişir M, Kandemir FM, Özkaraca M. Changes in arginase activity and AST enzyme levels in the cardiac and skeletal muscle and liver of lambs with white muscle disease. Turk J Vet Anim Sci 2015; 39: 611- 614.
31. Beytut E, Karatas F, Beytut E. Lambs with white muscle disease and selenium content of soil and meadow hay in the region of Kars, Turkey. Vet J 2002; 163: 214-217.
32. Mali VR, Palaniyandi SS. Regulation and therapeutic strategies of 4-hydroxy-2-nonenal metabolism in heart disease. Free Radic Res 2014; 48: 251-263.
33. Nakamura K, Kusano KF, Matsubara H, et al. Relationship between oxidative stress and systolic dysfunction in patients with hypertrophic cardiomyopathy. J Card Fail 2005 11: 117-123.